Jellyfish growth promoting peptide and preparation method thereof

A growth-promoting and jellyfish technology, which is applied in pharmaceutical formulations, toiletry preparations, cosmetic preparations, etc., can solve the problems of single product and inability to obtain functional jellyfish extracts, etc., to achieve appropriate molecular weight, improve cell regeneration ability, and safety high effect

Active Publication Date: 2020-04-17
GUANGZHOU KENENG COSMETICS RES CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Similarly, the cited document 2 requires the use of amylase or protease for enzymolysis, and its product is relatively simple. Even if it is fermented by fermentation strains, it is impossible to obtain a jellyfish extract with excellent performance.

Method used

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  • Jellyfish growth promoting peptide and preparation method thereof
  • Jellyfish growth promoting peptide and preparation method thereof
  • Jellyfish growth promoting peptide and preparation method thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0045] The first aspect of the present invention provides a kind of preparation method of jellyfish growth-promoting peptide, and described preparation method comprises:

[0046] Fermentation step: inoculate mixed strains in the slurry containing jellyfish tentacles for fermentation and culture to obtain a fermentation liquid;

[0047] Separation step: performing separation treatment on the fermentation broth to obtain the separation product containing the jellyfish growth-promoting peptide.

[0048] The jellyfish growth-promoting peptide of the present invention uses a multi-bacteria joint culture technique to improve the bioavailability of jellyfish, and the finally obtained jellyfish growth-promoting peptide has various target products. in particular:

[0049] jellyfish

[0050] Jellyfish are important plankton in the aquatic environment, such as: cnidaria phylum jellyfish class and cubic jellyfish class. The shape of the jellyfish's body is like a transparent umbrella. ...

Embodiment 1

[0116] Separate the jellyfish tentacles from the living jellyfish and clean them to remove surface dirt and foam; soak the cleaned jellyfish tentacles in deionized water for 24 hours and change the water every 2 hours; after soaking, remove the jellyfish tentacles Take it out and drain the water, put it into a freezer for freeze-drying, and crush it after freezing to obtain jellyfish tentacle particles with an average particle size of 60 meshes. The pulverized jellyfish tentacles particles were added to the deionized solution at a concentration of 2 g / mL, heated to 100° C. for 20 minutes to sterilize, and cooled to obtain a slurry containing jellyfish tentacles particles.

[0117] Bifidobacteria, Saccharomyces cerevisiae, and Bacillus natto were mixed and inoculated into the activation medium at an inoculation ratio of 1:2:1, and were cultured statically at 33°C for 24 hours for activation. Based on the total mass of the activation medium, the specific composition of the activ...

Embodiment 2

[0127] Separate the jellyfish tentacles from the living jellyfish and clean them to remove surface dirt and foam; soak the cleaned jellyfish tentacles in deionized water for 24 hours and change the water every 2 hours; after soaking, remove the jellyfish tentacles Take it out and drain the water, put it into a freezer to freeze-dry, and crush it after freezing to obtain jellyfish tentacles particles with an average particle size of 80 meshes. The pulverized jellyfish tentacles particles were added into deionized water at a concentration of 2 g / mL, heated to 100° C. for sterilization for 20 minutes, and cooled to obtain a slurry containing jellyfish tentacles particles.

[0128] Bifidobacterium, Saccharomyces cerevisiae, and Bacillus natto were mixed and inoculated into the activation medium at an inoculation ratio of 2:2:1, and were cultured statically at 34°C for 28 hours for activation. Based on the total mass of the activation medium, the specific composition of the activat...

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Abstract

The invention provides a jellyfish growth promoting peptide and a preparation method thereof. The preparation method of the jellyfish growth promoting peptide comprises the following steps: a fermentation step: inoculating a mixed strain into a slurry containing jellyfish tentacle particles, and carrying out fermentation culture to obtain a fermentation broth; a separation step: carrying out separation treatment on the fermentation broth to obtain a separation product containing the jellyfish growth promoting peptide; wherein the mixed strain comprise two or a combination of more than two of lactobacillus acidophilus, lactobacillus bulgaricus, bifidobacterium, pseudoalteromonas, candida utilis, saccharomyces cerevisiae, bacillus natto and bacillus subtilis. The jellyfish growth promoting peptide is more suitable in molecular weight, high in safety, excellent in stability and high in transdermal absorption rate. Furthermore, the jellyfish growth promoting peptide disclosed by the invention has a good promoting effect on growth or proliferation of cells in the skin, and can be used for improving the regeneration capacity of the cells in the skin and improving the elasticity of the skin.

Description

technical field [0001] The invention belongs to the technical field of marine organisms or cosmetics, and specifically relates to a preparation method of a jellyfish growth-promoting peptide, and the jellyfish growth-promoting peptide prepared by the preparation method and an application thereof. Background technique [0002] Jellyfish are marine organisms that belong to the phylum Cnidaria. In recent years, with environmental changes and climate changes caused by human activities, the frequency and scale of jellyfish outbreaks have gradually increased, and they have become a threat to disturb marine ecosystems. Long-term studies have found that jellyfish have well-developed gonads and strong reproductive and proliferative capabilities. Their high water and low carbon physical characteristics make them grow faster than other marine organisms. When jellyfish prey or defend against natural enemies, jellyfish tentacles will break and fall off, but new tentacles will grow from ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/04A23J3/04A23L33/18A23K20/147A61K8/64A61Q19/00A61K38/02A61P17/00
CPCA23J1/04A23J3/04A23L33/18A23K20/147A61K8/64A61Q19/00A61K38/02A61P17/00A23V2002/00A61K2800/85A23V2200/318A23V2250/55
Inventor 陈剑晖张慧黄建珍林娜妹李传茂张楚标曾伟丹张伟杰
Owner GUANGZHOU KENENG COSMETICS RES CO LTD
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