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Method for preparing carboxymethyl yeast glucan product and product obtained by using same

A technology of carboxymethyl dextran and yeast dextran, which is applied in the direction of cosmetic preparations, skin care preparations, cosmetics, etc., can solve the complex process of secondary alkali addition, poor safety of isopropanol, and large solvent consumption and other problems, to achieve excellent substitution effect, stable molecular weight, and save reagents

Active Publication Date: 2014-05-21
ANGELYEAST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Isopropanol is less safe, and it consumes a lot of solvent when it is prepared by slurry method, and the process of adding alkali twice is complicated
It can be seen that there are certain shortcomings in the above methods

Method used

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  • Method for preparing carboxymethyl yeast glucan product and product obtained by using same
  • Method for preparing carboxymethyl yeast glucan product and product obtained by using same
  • Method for preparing carboxymethyl yeast glucan product and product obtained by using same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Weigh 100kg of yeast dextran and place it in a kneader, add 150kg of analytically pure NaOH, 250kg of 70% ethanol, and control the temperature at 25°C for 90min. Then 100kg of chloroacetic acid was added, and 150kg of 70% ethanol was added, and the temperature was maintained at 30°C for 90 minutes, and then the temperature was lowered to complete the reaction.

[0048] Take out the carboxymethyl dextran from the kneader, add 300kg of 70% ethanol, wash and centrifuge to get the precipitate. Repeat the washing and centrifugation for 3 times in the above way, adjust the pH to neutral with hydrochloric acid, collect the precipitate, dry it in an oven until the water content is 5.8%, crush it and pack it to obtain carboxymethyl yeast dextran powder product.

Embodiment 2

[0050] Put 100kg of yeast glucan in a kneader, add 50kg of analytical pure NaOH, 50kg of 85% ethanol, and control the temperature at 30°C for 50min. Then 30kg of chloroacetic acid was added, and 50kg of 85% ethanol was added, and the temperature was maintained at 80°C for 60 minutes, and then the temperature was lowered to complete the reaction.

[0051]Take out the carboxymethyl dextran from the kneader, add 1000kg of 85% ethanol, wash and centrifuge to get the precipitate. Repeat the washing and centrifugation 3 times in the above way, adjust the pH to neutral with hydrochloric acid, collect the precipitate and dissolve it in water, adjust the weight percentage concentration to 2%, filter it with diatomaceous earth until the OD (600nm) value is 0.3, and use ultraviolet light Visible spectrophotometer measures the OD value at 600nm, and directly packs the carboxymethyl yeast glucan liquid product.

Embodiment 3

[0053] Put 100kg of yeast dextran in a kneader, add 100kg of analytical pure NaOH, 200kg of 70% ethanol, control the temperature at 20°C for 60 minutes, then add 80kg of chloroacetic acid and 100kg of 70% ethanol, and keep the temperature at 70°C for 60 minutes Cool down to end the reaction.

[0054] Take out the carboxymethyl dextran from the kneader, add 800kg of 70% ethanol, wash and centrifuge to get the precipitate. Repeat the washing and centrifugation for 5 times in the above-mentioned manner, adjust the pH to neutral with hydrochloric acid, collect the precipitate, dry it in an oven until the water content is 5.1%, crush it and pack it to obtain carboxymethyl yeast dextran powder product.

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Abstract

The invention relates to a method for preparing a carboxymethyl yeast glucan product and a product obtained by using the same. The preparation method comprises the following steps: adding alkali to yeast glucan according to the ratio that per 1kg of yeast glucan contains 12.5-37.5mol of hydroxyl; simultaneously adding ethanol according to the mass ratio of the yeast glucan to the ethanol being 1:(0.5-2.5), and reacting at 0-30 DEG C; adding a chloroacetic acid to the obtained solution according to the mass ratio of the yeast glucan to the chloroacetic acid being 1:(0.3-1.0), simultaneously adding ethanol according to the mass ratio of the yeast glucan to the ethanol being 1:(0.5-1.5), reacting at 30-80 DEG C and cooling; and adding ethanol in the obtained solution to wash, simultaneously neutralizing by using a hydrochloric acid, washing and centrifugally separating to obtain sediment carboxymethyl yeast glucan. By adopting the carboxymethyl yeast glucan product obtained by the method, the substitution degree is 0.3-1.0, the molecular weight is 100-1,000KD, and the carboxymethyl yeast glucan product is completely dissolved into water, and has the effects of moisturizing, preventing aging and the like when being applied to the field of cosmetics.

Description

technical field [0001] The invention relates to a method for preparing a carboxymethyl yeast glucan product and a product prepared therefrom. Background technique [0002] Yeast glucan is a functional polysaccharide existing in the cell wall of yeast. It has the effects of enhancing immunity, anti-infection, and anti-tumor. It can be used in food, cosmetics, animal nutrition and other fields. Dextran from yeast has a special three-dimensional helical structure and cannot be dissolved in water, thus limiting its application range in various fields. [0003] At present, there are reports in the literature that a large amount of isopropanol is used to prepare carboxymethyl dextran. In the process, a large amount of isopropanol is used as a solvent (slurry method), and the product prepared by adding alkali twice has a degree of substitution of 0.55. The degree of substitution refers to the average number of hydroxyl groups substituted by the reagent on each anhydroglucose unit,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/02A61K8/73A61Q19/08A61Q19/00
Inventor 张彦俞学锋李知洪余明华姚鹃张海波
Owner ANGELYEAST CO LTD
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