Turbot pathogenic strain and inactivated vaccine for ascites disease

An inactivated vaccine, turbot technology, applied in the directions of bacteria, microorganisms, biochemical equipment and methods, etc., can solve the problems of destroying ecological balance, drug residues, anorexia, etc., to curb the occurrence and prevalence, simple preparation process, and yield stable effect

Inactive Publication Date: 2012-07-04
THE FIRST INST OF OCEANOGRAPHY SOA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The treatment of turbot ascites disease currently mainly relies on antibiotics. Although it has a certain effect, it cannot fundamentally control the disease, and the fish body is prone to drug resistance, resulting in drug residues in the body and in the river water environment, destroying the Ecological balance, threatening human health and safety
In addition, fish schools are prone to anorexia symptoms during illness, so the method of feeding bait is not ideal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Inactivated vaccine of Acinetobacter ruckeri CCTCC M 2010372

[0030] 1. Preparation of inactivated vaccine of Acinetobacter ruckeri CCTCC M 2010372

[0031] First, the Acinetobacter ruckerii screened by the present invention was inoculated in 500ml high-salt common LB liquid medium (tryptone 10g / L, yeast extract juice 5g / L, sodium chloride 30g / L), and cultured with shaking at 28°C After 12 hours, the concentration of the bacterial liquid detected by the hemocytometer reaches 10 9 CFU / ml, or when the OD600 value reaches 0.6 or more, the culture is terminated, and the expanded culture medium of Acinetobacter ruckerii is obtained;

[0032] Add 25ml formaldehyde solution by volume ratio 0.5% afterward, inactivate 12 hours at room temperature to the expanded culture solution of bacterial strain, shake 3 times during this period; Obtain the inactivated bacterium solution of bacterial strain Acinetobacter ruckerii;

[0033] Finally, centrifuge the inactivated bac...

Embodiment 2

[0036] Embodiment 2: Acinetobacter ruckeri and pseudoalteromonas tetrodotoxin and / or Pseudomonas make the inactivated vaccine of mixed strain

[0037] 1. Preparation of inactivated vaccine

[0038] First, the Acinetobacter ruckerii screened by the present invention was inoculated in 500ml high-salt common LB liquid medium (tryptone 10g / L, yeast extract juice 5g / L, sodium chloride 30g / L), and cultured with shaking at 28°C After 12 hours, the concentration of the bacterial liquid detected by the hemocytometer reaches 10 9 CFU / ml, or when the OD600 value reaches 0.6 or more, the culture is terminated, and the expanded culture medium of Acinetobacter ruckerii is obtained;

[0039] Then one or both of the tetrodotoxin pseudoalteromonas or pseudomonas were inoculated in 500ml high-salt ordinary LB liquid medium (tryptone 10g / L, yeast extract juice 5g / L, sodium chloride 30g / L), cultured with shaking at 28°C for 16 hours, and the concentration of bacteria liquid detected by the hemo...

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PUM

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Abstract

The invention discloses a turbot pathogenic strain which is Acinetobacter lwoffii, wherein the collection number of the strain is CCTCC (China center for type culture collection) M2010372. The screened Acinetobacter lwoffii is used for preparing an inactivated vaccine. In addition, the Acinetobacter lwoffii, tetraodotoxin pseudoalteromonas and pseudomonas are used for preparing a bigeminy or trigeminy inactivated vaccine which is used for immunizing the turbot ascites disease. The preparation process of the inactivated vaccine for the ascites disease is simple, the yield is stable, the cost is low, and a large amount of inactivated vaccines with high immune effect can be obtained. The immune protective rate of the inactivated vaccine prepared by the method can reach 70%. The vaccine is a pure biological agent, has the characteristics of safety and environment friendliness, and dose not causes medicament residues and environment pollution; and by using the method, the generation and popularization of the turbot ascites disease are fundamentally suppressed, and a technical support is provided for preparing the novel inactivated vaccine for the turbot ascites disease.

Description

technical field [0001] The invention belongs to the field of microbial vaccine preparation, and in particular relates to a turbot pathogenic strain and an ascites disease inactivated vaccine. Background technique [0002] Turbot (Scophthalmas maximus) is a rare seawater fish species in Europe. It is native to the United Kingdom and belongs to the flounder family. One of the excellent marine cultured fish. my country has been introduced by the Yellow Sea Fisheries Research Institute for artificial breeding since 1992, and has reached a certain scale. It has gradually become an excellent aquaculture species in northern my country and the main target of factory farming, producing good economic and social benefits. However, with the expansion of the breeding range and the increase of the breeding density, as well as the degeneration of the species, the problem of turbot diseases has become increasingly prominent. Ascites disease is a comprehensive disease with wide prevalence ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/01
Inventor 郑风荣刘洪展殷树淼孙修勤张进兴
Owner THE FIRST INST OF OCEANOGRAPHY SOA
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