Rice peptide with whitening function and preparation method thereof

A rice peptide and whitening technology, which is applied to the preparation method of peptide, medical preparations containing active ingredients, chemical instruments and methods, etc., can solve the problems that affect the research process of deep processing of rice raw materials, and there is no more research. Excellent free radical scavenging ability, significant antioxidant function and whitening function, and the effect of inhibiting melanin synthesis

Active Publication Date: 2021-04-30
CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the research, development and application of rice peptides are mostly aimed at providing nutrients that are more easily absorbed by the human body, and about maintaining and improving the health of the body. The exploration of the enzymatic hydrolysis process of rice protein is also more concerned about the conventional efficacy of rice protein. As a result, as to whether there will be some peptides that not only promote absorption in the enzymatic hydrolysis products, and whether the application range of rice peptides can be expanded by obtaining peptides with other functions, there are no more studies and reports. It also affected the research process of deep processing of rice raw materials

Method used

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  • Rice peptide with whitening function and preparation method thereof
  • Rice peptide with whitening function and preparation method thereof
  • Rice peptide with whitening function and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] The rice peptide of the present embodiment is prepared according to the following method:

[0073] 1. Put 500g of rice protein powder (80% protein content, 60 mesh) into the steam explosion reaction tank, feed steam, control the pressure at 0.65Mpa, maintain it for 100s, and then release the pressure at 5.0 milliseconds to obtain rice steam explosion granules;

[0074] Add 5L of pure water to the rice steam explosion granules to obtain a mixed solution;

[0075] 2. Heat the mixture to 50°C, add 10 mL of concentrated hydrochloric acid to adjust the pH to 3.0, then add 2 g of pepsin for the first enzymatic hydrolysis, and obtain the first enzymatic hydrolysis after 3 hours of enzymatic hydrolysis;

[0076] Then add 4 g of sodium hydroxide to the first enzymolysis solution to adjust the pH to 7.0, then add 1 g of papain for the second enzymolysis, and obtain the second enzymolysis solution after 1 hour of enzymolysis;

[0077] Add 0.5 g of aminopeptidase to the second enz...

Embodiment 2

[0099] The rice peptide of the present embodiment is prepared according to the following method:

[0100] 1. Put 200g of rice protein powder (80% protein content, 60 mesh) into the steam explosion reaction tank, feed steam, control the pressure at 0.6Mpa, maintain it for 100s, and then release the pressure at 5.0 milliseconds to obtain rice steam explosion granules;

[0101] Add 2L of pure water to rice steam explosion granules to obtain a mixed solution;

[0102] 2. Heat the mixture to 50°C, add 4 mL of concentrated hydrochloric acid to adjust the pH to 3.0, add 1 g of pepsin for the first enzymatic hydrolysis, and obtain the first enzymatic hydrolysis after 3 hours of enzymatic hydrolysis;

[0103] Then add 1.5 g of sodium hydroxide to the first enzymolysis solution to adjust the pH to 6.5, then add 0.4 g of papain to carry out the second enzymolysis, and obtain the second enzymolysis solution after 1.5 hours of enzymolysis;

[0104] Add 0.2 g of aminopeptidase to the secon...

Embodiment 3

[0118] The rice peptide of the present embodiment is prepared according to the following method:

[0119] 1. Put 100g of rice protein powder (80% protein content, 60 mesh) into the steam explosion reaction tank, feed steam, control the pressure at 0.6Mpa, maintain it for 100s, and then release the pressure at 5.0 milliseconds to obtain rice steam explosion particles;

[0120] Add 5L of pure water to rice steam explosion granules to obtain a mixed solution;

[0121] 2. Raise the temperature of the mixture to 50°C, add 2 mL of concentrated hydrochloric acid to adjust the pH to 3.0, then add 0.3 g of pepsin for the first enzymatic hydrolysis, and obtain the first enzymatic hydrolysis solution after 3 hours of enzymatic hydrolysis;

[0122] Then add 1.0 g of sodium hydroxide to the first enzymolysis solution to adjust the pH to 7.5, then add 0.2 g of papain to carry out the second enzymolysis, and obtain the second enzymolysis solution after 1.5 hours of enzymolysis;

[0123] Add...

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Abstract

The invention provides a rice peptide with a whitening function as well as a preparation method and an application thereof. The rice peptide at least comprises peptide fragments GS, GL and GF, based on the mass of the rice peptide, the content of the peptide fragment GS is greater than or equal to 200.00 mg/100g, the content of the peptide fragment GL is greater than or equal to 200.00 mg/100g, and the content of the peptide fragment GF is greater than or equal to 100.00 mg/100g. The rice peptide containing the three peptide fragments of GS, GL and GF can significantly remove DPPH free radicals and OH free radicals, reduce the ROS content, melanin synthesis rate and tyrosinase activity in human skin melanocytes, and has strong antioxidant ability and whitening ability.

Description

technical field [0001] The invention relates to a rice peptide with whitening function and its preparation method and application, belonging to the technical field of rice deep processing. Background technique [0002] Rice is very nutritious and is the main food for people in most parts of China. Rice is mainly composed of starch and protein, the content is about 80% and 8% respectively. Since the biological value of protein in rice is equivalent to that of soybean, and the content of lysine and threonine is very rich, rice is usually processed to extract the protein. [0003] Rice protein refers to the protein extracted from rice, which is a powder substance formed by crushing, purification, drying and other processes. It has excellent nutritional quality and is recognized as a high-quality vegetable protein among cereal proteins. In rice protein, albumin accounts for 2-5% of the total, globulin accounts for 2-10% of the total, alcohol-soluble protein accounts for 1-5% o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/01A61K8/64A61P17/16A61P17/18A61P39/06C12P21/06C07K1/36C07K1/34C07K1/18A61Q19/02A61Q19/08A61K36/899
CPCA61K38/011A61P17/18A61P39/06A61P17/16A61K8/645A61Q19/02A61Q19/08C12P21/06A61K2236/37A61K2236/19A61K36/899A61K8/64A61K38/01C07K1/34C07K1/36C07K1/18C07K5/06C07K1/14A61K38/05
Inventor 蔡木易谷瑞增陈亮马勇张海欣魏颖刘艳王雨晴张瑞雪马永庆刘文颖秦修远
Owner CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
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