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Ray chondroitin sulfate and extraction method thereof

A technology of chondroitin sulfate and chondroitin, which is applied in the direction of pharmaceutical formulations, organic active ingredients, anti-toxic agents, etc., can solve the problems of incomplete hydrolysis of collagen and mucin, pollution of the environment, etc., and achieve strong ability to scavenge free radicals, strong The effect of antioxidant capacity

Inactive Publication Date: 2020-06-09
HEBEI AGRICULTURAL UNIV.
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of the above methods cannot completely hydrolyze the collagen and mucin in the cartilage, and the high concentration of lye can easily cause the degradation of CS and seriously pollute the environment

Method used

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  • Ray chondroitin sulfate and extraction method thereof
  • Ray chondroitin sulfate and extraction method thereof
  • Ray chondroitin sulfate and extraction method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Comparison of the pros and cons of different skate chondroitin sulfate extraction methods in embodiment 1

[0042] Concentrated alkali-concentrated salt method, dilute alkali-enzymatic hydrolysis method and ultrasonic-assisted alkali-salt-enzymatic hydrolysis method were used to extract cartilage of ray cartilage, and the extraction rate of chondroitin sulfate, glucuronic acid content and appearance quality were used as indicators to compare each Pros and cons of extraction methods.

[0043]1. Extraction process of ray chondroitin sulfate by concentrated alkali-concentrated salt method

[0044] Ray cartilage powder → 10% NaOH, 20% NaCl → 40°C extraction → adjust the pH of the filtrate to 7-8 → ethanol precipitation → centrifuge to take the precipitate → freeze-drying → CS;

[0045] 2. Extraction process of ray chondroitin sulfate by dilute alkali-enzymatic hydrolysis

[0046] Ray cartilage powder → 2% NaOH, 2% NaCl → 40 ℃ alkali salt extraction → adjust pH to 9 → 50 ℃...

Embodiment 2

[0053] Example 2 Ultrasonic assisted alkali salt-enzymolysis method to extract chondroitin sulfate single factor process parameter test

[0054] Taking the extraction rate of CS and the content of glucuronic acid as the evaluation index, the six effects of lye concentration, extraction temperature, ultrasonic extraction time, alkaline protease addition, enzymolysis temperature and enzymolysis time on the extraction of ray chondroitin sulfate were respectively evaluated. The key process parameters of the effect are subjected to a single factor test, and the factor levels of the single factor test are shown in Table 2.

[0055] Table 2 Factor level table of single factor experiment

[0056]

[0057] The formula for calculating the extraction rate of chondroitin sulfate is In the formula: W—chondroitin sulfate extraction rate, %; m—freeze-dried chondroitin sulfate product quality, g; 5—mass of skate cartilage powder, g.

Embodiment 21

[0058] Embodiment 2.1 lye concentration single factor test

[0059] Weigh 5g of ray cartilage powder into a beaker for each group of tests, add 2%NaCl-1%NaOH, 2%NaCl-2% respectively according to the design of Table 2-3 with a solid-liquid ratio of 1:6g / mL Add NaOH, 2% NaCl-3% NaOH, 2% NaCl-4% NaOH aqueous solution, stir while adding to make it fully mixed, and ultrasonically extract at 40°C for 180min. After taking it out, filter it with double-layer gauze, collect the filtrate, adjust the pH to 11 after cooling, add 0.5% (mass volume ratio) alkaline protease, and shake at 45°C for 12h. After taking out, inactivate the enzyme at 90°C for 10min, centrifuge at 5000r / min for 10min, take the supernatant, precipitate with ethanol with a final concentration of 75%, let stand at 4°C for 24h, collect the precipitate by centrifugation and freeze-dry to obtain CS polysaccharide. The CS was weighed in time, stored at 4°C under dry conditions, and its content was to be determined.

[00...

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Abstract

The invention discloses ray chondroitin sulfate and an extraction method thereof. An ultrasonic-assisted alkali salt-enzymolysis method is adopted, and the optimal process conditions are as follows: the concentration of alkali liquor is 2.3%, the extraction temperature is 43 DEG C, the enzyme addition amount is 0.7%, the ultrasonic extraction time is 180 minutes, the enzymolysis temperature is 45DEG C, the enzymolysis time is 12 hours, the optimal extraction rate can reach 43.32%, and the purity reaches 94%. The characteristic components of the ray CS are 4-monosulfated disaccharide CS-A, 6-monosulfated disaccharide CS-C and 2, 6-disulfated disaccharide CS-C, the ray CS is different from shark chondroitin in the aspect of A / C (CS-A / CS-C content ratio), and the ray CS not only has the potential of serving as a good shark CS substitute, but also shows physiological activity and functions different from those of shark CS due to the outstanding characteristics. The content of sulfate radicals in the ray chondroitin sulfate reaches 17.18%, the ray chondroitin sulfate is highly sulfated chondroitin polysaccharide, the oxidation resistance of the ray chondroitin sulfate is obviously higher than that of CS with a low sulfation degree, and the ray chondroitin sulfate has high free radical scavenging capacity.

Description

technical field [0001] The invention relates to the technical field of bioactive substances, in particular to skate chondroitin sulfate and an extraction method thereof. Background technique [0002] Chondroitin Sulfate (CS) is a glycosaminoglycan commonly found in animal cartilage and connective tissue. Free radicals in the body, relieve arthritis, etc. Chondroitin sulfate can also be used as a food additive for emulsification, moisturizing and deodorization of food, and in cosmetics to regulate skin cell metabolism and maintain skin moisture. [0003] The basic unit of CS is a disaccharide unit (GlcAβ-1,3GalNAc) formed by β-D-glucuronic acid and N-acetyl-β-D-galactosamine linked by β-1,3 glycosidic bonds. The disaccharide units are connected repeatedly and alternately through β-1,4 glycosidic bonds to form a linear biomacromolecule with a degree of polymerization of 40-70 and a molecular weight of generally 10-80kDa. According to the position and number of hydroxyl grou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08A61K31/737A61P39/06
CPCA61K31/737A61P39/06C08B37/0003C08B37/0069
Inventor 桑亚新涂学宏卢海强周畅孙纪录亢春雨
Owner HEBEI AGRICULTURAL UNIV.
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