Extraction method of nonextractable polyphenol in blueberry

An extraction method and extraction technology, applied in the field of extraction of non-extractable polyphenols in blueberries, can solve the problems of low NEPP activity, increased extraction difficulty, and many processing steps, and achieve low environmental pollution, mild method, and simple operation steps Effect

Active Publication Date: 2017-06-30
TIANJIN AGRICULTURE COLLEGE
View PDF1 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Blueberries contain high NEPP. Due to the limitation of the extraction process, non-extractable polyphenols have been ignored by researchers; and NEPP in blueberries is conjugated with dietary fiber protein, making it more difficult to extract
At present, most of the extraction methods of NEPP in blueberries are extracted by physical assistance methods. The extraction method has many processing steps, the extraction efficiency is low, and the activity of the extracted NEPP is low.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The specific steps are as follows:

[0051] 1. Preparation of blueberry residue: collect the residue after squeezing the blueberry juice and dry it at room temperature to obtain blueberry residue;

[0052] 2. Preparation of blueberry residue powder: crush the blueberry residue and pass through an 80-mesh sieve to obtain blueberry residue powder;

[0053] 3. Acid soaking: Put 10g of blueberry dregs powder into a 2L glass beaker, add 1000ml of 0.5mol / L hydrochloric acid solution for soaking, soaking time is 12.5 hours, soaking temperature is 3℃, to obtain acid soaking solution.

[0054] 4. Alkaline soaking: Measure 50ml of saturated sodium hydroxide solution with a graduated cylinder, add it to the acid soaking solution, and continue soaking for 3.5 hours at a temperature of 3°C to obtain the alkaline soaking solution. The concentration of saturated sodium hydroxide is 20mol / L, and there is 20×50÷1000=1mol sodium hydroxide. After reacting with 0.5mol hydrochloric acid, 0.5mol sod...

Embodiment 2

[0062] The specific steps are as follows:

[0063] 1. Preparation of blueberry residue: collect the residue after squeezing the blueberry juice and dry it at room temperature to obtain blueberry residue;

[0064] 2. Preparation of blueberry residue powder: crush the blueberry residue and pass through a 120-mesh sieve to obtain blueberry residue powder;

[0065] 3. Acid soaking: Put 10g of blueberry dregs powder into a 2L glass beaker, add 1200ml of 0.6mol / L hydrochloric acid solution for soaking, soaking time is 11.5 hours, soaking temperature is 5℃, to obtain acid soaking solution.

[0066] 4. Alkaline soaking: Measure 71ml of saturated sodium hydroxide solution with a graduated cylinder, add it to the acid soaking solution, and continue soaking for 2.5 hours at a soaking temperature of 5°C to obtain an alkaline soaking solution. The concentration of saturated sodium hydroxide is 20mol / L, and there is 20×71÷1000=1.42mol sodium hydroxide. After reacting with 1.2×0.6=0.72mol hydrochlor...

Embodiment 3

[0074] The specific steps are as follows:

[0075] 1. Preparation of blueberry residue: collect the residue after squeezing the blueberry juice and dry it at room temperature to obtain blueberry residue;

[0076] 2. Preparation of blueberry residue powder: crush the blueberry residue, pass a 100-mesh sieve to obtain blueberry residue powder;

[0077] 3. Acid soaking: Put 10g of blueberry dregs powder into a 2L glass beaker, add 1100ml of 0.5mol / L hydrochloric acid solution for soaking, soaking time is 12 hours, soaking temperature is 4℃, to obtain acid soaking solution.

[0078] 4. Alkali soaking: Measure 54ml of saturated sodium hydroxide solution with a graduated cylinder, add it to the acid soaking solution, and continue soaking for 3 hours at a temperature of 4°C to obtain the alkaline soaking solution. The concentration of saturated sodium hydroxide is 20mol / L, with 20×54÷1000=1.8mol sodium hydroxide. After reacting with 0.55mol hydrochloric acid, 0.53mol sodium hydroxide is left...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to an extraction method of nonextractable polyphenol in blueberry, and belongs to the technical field of extraction of plant polyphenol. The extraction method comprises the followings steps: preparing blueberry residue, preparing blueberry residue powder, performing acid solution soaking, performing alkali solution soaking, performing enzymolysis, centrifugation, extraction and collecting extractive. The extraction method is simple in operation steps, the operation steps can be performed in one container, and the extractive is not required to be repeatedly collected; weak concentration acid and alkali are adopted to treat materials, ethyl acetate and ethyl alcohol mixed liquor can be utilized for extracting, the method is mild, the bioactivity of the nonextractable polyphenol can be furthest reserved, the extraction ratio is improved, and the environmental pollution is very small.

Description

Technical field [0001] The invention belongs to the technical field of plant polyphenol extraction, and specifically relates to an extraction method of non-extractable polyphenols in blueberries. Background technique [0002] Blueberry, English name: Blueberry, which means blue berry, belongs to the rhododendron family, a plant of the lingonberry genus, the fruit is dark blue, covered with hoarfrost, nearly round, delicate flesh, sweet and sour. The blueberry fruit is rich in nutrients, which have the functions of preventing cranial nerve aging, protecting eyesight, strengthening the heart, anti-cancer, softening blood vessels, and enhancing human immunity. It has high nutritional content and is known as the "golden berry". One of the five recommended healthy fruits. In addition to the conventional sugar, acid and vitamin C, blueberry fruit is rich in vitamin E, vitamin A, vitamin B, superoxide dismutase (SOD), arbutin, protein, anthocyanin, dietary fiber, and rich potassium , ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/105
CPCA23V2002/00A23V2200/3202A23V2250/2106A23V2250/2132
Inventor 张爱琳许程剑苗颖张登科郝程列
Owner TIANJIN AGRICULTURE COLLEGE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap