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A kind of arctic marine bacterium and its application

A technology of marine bacteria and strains, applied in the field of microorganisms, can solve the problems of low development and utilization, and achieve the effects of good application potential, good viscoelasticity, and good temperature stability

Active Publication Date: 2016-06-29
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there have been some researches at home and abroad on the exopolysaccharides secreted by marine microorganisms, the overall degree of development and utilization is still very low.

Method used

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  • A kind of arctic marine bacterium and its application
  • A kind of arctic marine bacterium and its application
  • A kind of arctic marine bacterium and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] An arctic marine bacterium (Polaribacter sp.) SM1127 strain was preserved in the China Center for Type Culture Collection on September 21, 2013, address: Wuhan University, Luojia Mountain, Wuchang, Wuhan, China, strain preservation number: CCTCCM2013437.

[0070] The 16SrRNA gene of the Arctic marine bacteria was extracted for species identification. After comparing its gene sequence in the NCBI database, it was found that the strain with high similarity to Arctic marine bacteria was PolaribactersejongensisKOPRI21160 T (similarity: 99.13%), PolaribacterbutkevichiiKMM3938 T (similarity: 98.67%) and Polaribacterirgensii23-P T (Similarity: 97.43%). Thus, Arctic marine bacteria belong to the genus Polaribacter.

Embodiment 2

[0072] The application of Arctic marine bacterium (Polaribacterse jongensissp.) SM1127 strain described in embodiment 1 in the preparation of Arctic marine bacterial exopolysaccharide, the steps are as follows:

[0073] (1) Streak-inoculate the Arctic marine bacteria (Polaribacterse jongensissp.) SM1127 strain in a solid medium, activate and cultivate it at 20°C for 2 days, then inoculate it in 100mL liquid seed medium, and inoculate it at 20°C and 200rpm Shake culture for 1 day, then inoculate in 5L fermentation medium at 2% (v / v) inoculum, and culture continuously for 8 days at 11.3°C and dissolved oxygen saturation of 30-100%. The pH is 7.5, and 200mL, 200mL and 100mL of industrial glucose solution with a concentration of 625g / L prepared by industrial glucose and artificial seawater are added on the 3rd day, 4th day and 5th day of cultivation respectively to obtain a fermentation broth;

[0074](2) Add 2 times the volume of absolute ethanol to the fermentation broth prepare...

Embodiment 3

[0079] The application of Arctic marine bacteria (Polaribactersp.) SM1127 strain described in Example 1 in the preparation of Arctic marine bacterial exopolysaccharide, the steps are as follows:

[0080] (1) Streak inoculation of Arctic marine bacteria (Polaribactersp.) SM1127 strain in solid medium, activate culture at 15°C for 3 days, and then inoculate in 100mL liquid seed medium, at 15°C, 200rpm Shake culture for 2 days, then inoculate in 5L fermentation medium with an inoculum size of 3% (v / v), and culture continuously for 6 days at 15°C and dissolved oxygen saturation of 60-80%. The pH is 8.0, and 200mL, 200mL and 100mL of industrial glucose solutions with a concentration of 625g / L prepared by industrial glucose and artificial seawater are added on the 3rd day, 4th day and 5th day of cultivation respectively to prepare fermentation broth;

[0081] (2) Add 3 times the volume of absolute ethanol to the fermentation broth prepared in step (1), alcohol precipitation at 6°C f...

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Abstract

The invention relates to a polaribacter sp. and an application. Luojia Mountain in Wuchang district, Wuhan, China, and the preservation number is CCTCC No. M2013437. The bacterial strain can secrete a lot of polaribacter sp extracellular polysaccharide. The polysaccharide has good rheology property, is capable of absorbing moisture, preserving moisture, removing activity of organic free radical, hydroxyl radical and oxyradical, and has the characteristics of non toxicity and no stimulation.

Description

technical field [0001] The invention relates to an arctic marine bacterium and its application, belonging to the technical field of microorganisms. Background technique [0002] Microbial exopolysaccharides are secondary metabolites of microorganisms, which are soluble polysaccharides and polysaccharide complexes that are secreted outside the cell wall during the growth of microorganisms and separated from the bacteria. There are many kinds of microbial exopolysaccharides, mainly composed of monosaccharides such as D-glucose, D-galactose and D-mannose. They have excellent properties that plant polysaccharides do not have: short production cycle, not limited by seasons, regions and pest conditions, strong market competitiveness and broad development prospects. Microbial polysaccharides have extensive application value in many fields of industrial production and life, such as microbial flocculants, biochemical profile control agents in microbial oil recovery, food additives, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P19/04C12R1/01
Inventor 张玉忠孙美玲陈秀兰石梅张熙颖宋晓妍解彬彬苏海楠秦启龙周百成
Owner SHANDONG UNIV
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