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Green tea fermentation filtrate and preparation method and application thereof

A technology of green tea fermentation and filtrate, which is applied in the field of green tea fermentation filtrate and its preparation, to achieve good color and clarity, maintain color and clarity, and inhibit the effect

Active Publication Date: 2019-04-30
ANGEL NUTRITECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the active ingredient tea polyphenols in green tea is used as an efficacy factor in cosmetics, but there is no report on the use of green tea fermented filtrate as a cosmetic or as a food application.

Method used

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  • Green tea fermentation filtrate and preparation method and application thereof
  • Green tea fermentation filtrate and preparation method and application thereof
  • Green tea fermentation filtrate and preparation method and application thereof

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preparation example Construction

[0062] The invention provides a preparation method of green tea fermentation filtrate, which comprises activating, purifying and expanding the culture of yeast to obtain a logarithmic fermentation bacteria liquid; soaking green tea in water to obtain a green tea soaking liquid, Then, nitrogen source and carbon source are added to the green tea steeping liquid for sterilization; fermenting bacteria liquid is inoculated for fermentation, followed by filtration and sterilization to obtain green tea fermentation filtrate.

[0063] Wherein, in a preferred embodiment of the present invention, the present invention provides a kind of preparation method of green tea fermentation filtrate, comprises the following steps:

[0064] (1) Strain activation: the yeast is put into the liquid medium for activation culture;

[0065] (2) Strain purification: Dilute the activated bacterial liquid described in step (1) to obtain a single colony;

[0066] (3) Bacterial classification enlargement cu...

Embodiment 1

[0086] (1) Preparation of green tea fermentation filtrate

[0087] Adopt the following method to prepare green tea fermentation filtrate:

[0088] (1) Strain activation: pick one ring of Saccharomyces cerevisiae Hansen Z2.1 colony and put it into the YPD liquid medium containing 0.9% by weight of yeast extract, 1.5% by weight of peptone, and 1.8% by weight of glucose, Put it in a shaker to activate the strain;

[0089] (2) Strain purification: the activated bacterial solution in step (1) is diluted and plated to obtain a single colony;

[0090] (3) Expanded culture of strains: Pick a single colony in the plate in step (2) and inoculate it into the YPD liquid medium containing 0.9% by weight of yeast extract, 1.5% by weight of peptone, and 1.8% by weight of glucose, and culture it on a shaking table at 28°C , when OD=1.0, that is, when the strain is in the logarithmic phase, the fermented bacteria liquid is obtained;

[0091] (4) Inoculation fermentation: add 5 g of the ferm...

Embodiment 2

[0133] (1) Preparation of green tea fermentation filtrate

[0134] Adopt the following method to prepare green tea fermentation filtrate:

[0135] (1) Strain activation: pick one ring of Saccharomyces cerevisiae Hansen Z2.1 colony and put it into the YPD liquid medium containing 0.9% by weight of yeast extract, 1.5% by weight of peptone, and 1.8% by weight of glucose, Put it in a shaker to activate the strain;

[0136] (2) Strain purification: the activated bacterial solution in step (1) is diluted and plated to obtain a single colony;

[0137](3) Expanded culture of strains: Pick a single colony from the plate in step (2) and inoculate it into the YPD liquid medium containing 0.9% by weight of yeast extract, 1.5% by weight of peptone, and 1.8% by weight of glucose, and cultivate it on a shaking table at 30°C , when OD=1.0, that is, when the strain is in the logarithmic phase, the fermented bacteria liquid is obtained;

[0138] (4) Add 20 g of the fermented bacteria liquid ...

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Abstract

The invention relates to the technical field of microbial fermentation, in particular to a green tea fermentation filtrate and a preparation method and an application thereof. The present invention provides a green tea fermentation filtrate which is a fermentation culture product comprising a green tea extract, a carbon source, a nitrogen source, and a raw material mixture of yeast. The inventionalso provides the preparation method of the green tea fermentation filtrate, which comprises the following steps: (1) strain activation, (2) strain purification, (3) strain expanded culture, (4) inoculation fermentation, (5) ) clarification treatment, and (6) sterilization. The green tea fermentation filtrate of the invention does not contain any chemical components, and no additional enzyme or other processing aid is added during the production process, the production cost is small, and the production steps are simple. The product has the pleasant fermentation scent and good green color and clarity. The fermentation filtrate has the strong ability to scavenge free radicals, and the effect of inhibiting tyrosinase is remarkable.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a green tea fermentation filtrate and a preparation method and application thereof. Background technique [0002] Green tea, also known as "non-fermented" tea, is made from the young shoots of tea trees through processes such as killing, rolling and drying. Tea polyphenols are the general term for polyhydric phenolic compounds, and are the main functional components in green tea, accounting for 22-30% of the dry weight of tea leaves. The molecular structure of polyphenols contains multiple benzene rings, and each benzene ring has two or three hydroxyl groups, which can be used as hydrogen donors, have strong antioxidant effects, and can significantly inhibit free radical damage. At the same time, there are other bioactive substances in green tea, which have remarkable effects in skin care and food. [0003] The application number is 2011102710031667.4, which disc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/02C12G3/026A61Q19/00A23F3/16A23L2/38C12R1/865
CPCA61Q19/00A23F3/166A23L2/382A23L33/10A61K8/97C12G3/02C12P1/02A23V2002/00A61K2800/85C12R2001/865C12N1/185A23V2200/30
Inventor 彭宁俞学锋李知洪姚鹃张彦张海波陈智仙杨树金冯玖刘阳峰
Owner ANGEL NUTRITECH CO LTD
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