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Method for preparing peony stamen extract

A peony flower and stamen technology, applied in the field of new food resources development, can solve the problems of waste, discarded peony stamens, underutilization of peony stamens, etc., to achieve golden color, increase immunity, and unique and attractive fragrance Effect

Inactive Publication Date: 2012-07-25
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the stamens of peony flowers have not been fully utilized
After the annual flowering period, a large number of peony stamens are discarded together with the plants, resulting in huge waste

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 Study on the Extraction Medium of Functional Substances in Peony Stamens

[0016] Take 4 parts of 0.500g peony flower stamens, put them in four small beakers, add 8mL of pure water, ethanol, petroleum ether, ethyl acetate respectively, soak for 1 hour, observe the color of the solution, and you can find the yellow color displayed by the pure aqueous solution> Ethanol solution>petroleum ether solution>ethyl acetate solution. The four solutions obtained by filtration were scanned at full wavelength, and if flat-topped peaks appeared, the operation was repeated after dilution until there was no flat-topped peak in the full-wavelength scanning graph. After analyzing the results of 4 samples, it was concluded that pure water was the best solvent. The ratio of peony stamens to purified water is 1:16 (m / v).

Embodiment 2

[0017] Example 2 High Performance Liquid Chromatography Separation of Functional Substances in Peony Stamens and Study on Preparation Conditions of Semi-preparative Chromatography

[0018] The detection wavelength was determined to be 266nm from the full wavelength scanning results. Set the single-task time to 90 minutes, the injection volume to 10 μL, the flow rate to 1 mL / min, and the temperature to room temperature. Try methanol:water ratios of 5:5, 4:6, 3:7, 2:8, and 9:1 mobile phases. , Add 0.1% glacial acetic acid to the water, mix the mobile phase before use, and ultrasonicate for 10 minutes to remove the air bubbles. After analyzing the liquid chromatograms, it was found that 3:7 was the optimal ratio, and no peaks appeared after 60 minutes. After further experimenting with the ratios of 3.5:6.5 and 2.5:7.5, it was found that the methanol:water ratio of 3:7 is still the best mobile phase ratio. Then draw the final liquid chromatographic conditions for various sample ...

Embodiment 3

[0019] Example 3 Screening of Different Column Materials for Separation and Purification of Functional Substances in Peony Stamens

[0020] The purified water crude extract was extracted with petroleum ether and ethyl acetate, and the aqueous phase waited for column chromatography to be loaded. This technology adopts polyamide, macroporous resin AB-8, and macroporous resin XAD-16 three column chromatography materials to carry out comparative experiments on separation and purification effects. The experiment adopts the wet method for sample loading. Take 20mL concentrated sample and load it, and then use purified water, 30% ethanol, 60% ethanol and absolute ethanol to elute in sequence. The elution rate is controlled at 40-50 drops / min. The eluting pigment band is connected with the test tube, and the concentration and number of the eluent are indicated, and the separation effect is compared by high performance liquid chromatography. The test results show that the macroporous ...

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PUM

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Abstract

The invention relates to a method for preparing a peony stamen extract, and particularly researches an extraction, separation and purification method for functional substances with antioxidant ability in peony stamens and prepares an extract with antioxidant ability. When the peony stamen extract prepared by the method is used in detection of scavenging of ABTS (2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate)) free radicals, the inhibitory concentration (IC50) of the extract is 0.914mg / mL when 50% of ABTS free radicals are scavenged, and therefore, the peony stamen extract prepared by the method has antioxidant ability and is beneficial for delaying senescence, enhancing immunity, beautifying and caring skin and the like.

Description

(1) Technical field [0001] The invention relates to a method for preparing peony stamen extract, specifically studies the extraction, separation and purification method of functional substances with anti-oxidation ability in peony stamens, and prepares the extract with anti-oxidation ability, which belongs to new food resources development field. (2) Background technology [0002] peony Paeonia suffruticosa , Paeoniaceae, Paeoniaceae, originate in the mountainous areas of Qinling and Daba Mountains in western China. Peony is a unique woody and precious flower in my country. It has large and colorful flowers, elegant and luxurious, rich and dignified, rich fragrance, and various varieties. It is known as "National Beauty and Heavenly Fragrance" and "King of Flowers". A symbol of wealth, auspiciousness, and prosperity. Luoyang peony and Heze peony are the most famous. In addition to the ornamental value of peony, its petals have been used as food for more than a thousan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/29A23L33/00
Inventor 张仁堂谷端银
Owner SHANDONG AGRICULTURAL UNIVERSITY
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