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Low-molecular-weight blackberry polysaccharide and preparation method thereof

A low molecular weight, weight average molecular weight technology, applied in the field of blackberry extract, can solve the problems of weak activity, high molecular weight of polysaccharides, polysaccharide structure and physical and chemical properties hindering biological activity, etc., and achieves easy operation, simple equipment, cost-effective green environmental protection and high efficiency Effect

Active Publication Date: 2019-07-30
珠海中美普莱健康科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In recent years, people have conducted in-depth research on the functional activities of plant polysaccharides, and found that the structure of polysaccharides has an important impact on their biological activities. Polysaccharides isolated and purified from natural plants have large molecular weight and weak activity

Method used

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  • Low-molecular-weight blackberry polysaccharide and preparation method thereof
  • Low-molecular-weight blackberry polysaccharide and preparation method thereof
  • Low-molecular-weight blackberry polysaccharide and preparation method thereof

Examples

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Effect test

Embodiment 1

[0065] A method for preparing low-molecular-weight blackberry polysaccharides comprises the following steps: selecting fresh blackberry fruit, cleaning, drying at 45° C. for 24 hours, crushing and passing through a 60-mesh sieve to obtain blackberry powder.

[0066] Accurately weigh 50g of blackberry powder, add 3 times the mass of blackberry powder with 95% (volume percentage) ethanol, heat and stir at 75°C for 4h, centrifuge and filter, and dry the filter residue at 45°C for 24h.

[0067] Add 30 times the mass of blackberry filter residue to the dried blackberry filter residue, add and stir for 4 hours at 85°C, collect the filtrate after centrifuging at 5000g for 10 minutes, repeat the operation twice, combine the two filtrates and concentrate under reduced pressure to 200ml , add Sevag reagent (n-butanol:chloroform=1:4, v / v) of 1 / 4 concentrated solution volume, shake at 200r / min for 20min, collect the supernatant after centrifuging at 5000g for 10min, and then deproteinize b...

Embodiment 2

[0072] A method for preparing low-molecular-weight blackberry polysaccharides comprises the following steps: selecting fresh blackberry fruit, cleaning, drying at 45°C for 12 hours, crushing and passing through a 60-mesh sieve to obtain blackberry powder. Accurately weigh 50g of blackberry powder, add 3 times the mass of blackberry powder with 95% (volume ratio) ethanol, heat and stir at 75°C for 4h, centrifuge and filter, and dry the filter residue at 45°C for 12h. Add 30 times the mass of blackberry filter residue to the dried blackberry filter residue, add and stir at 85°C for 2 hours, collect the filtrate after centrifuging at 5000g for 10 minutes, repeat the operation twice, combine the two filtrates and concentrate under reduced pressure to 200ml , add Sevag reagent (n-butanol:chloroform=1:4, v / v) of 1 / 4 concentrated solution volume, shake at 200r / min for 20min, collect the supernatant after centrifuging for 10min, then deproteinize by Sevag method, repeat 12 times. The...

Embodiment 3

[0074]A method for preparing low-molecular-weight blackberry polysaccharides comprises the following steps: selecting fresh blackberry fruit, cleaning, drying at 60°C for 24 hours, crushing and passing through a 100-mesh sieve to obtain blackberry powder. Accurately weigh 50g of blackberry powder, add 95% ethanol 4 times the mass of blackberry powder, heat and stir at 75°C for 4h, centrifuge and filter, and dry the filter residue at 60°C for 24h. Add 40 times the mass of blackberry filter residue to the dried blackberry filter residue, add and stir for 4 hours at 85°C, collect the filtrate after centrifugation, repeat the operation twice, combine the two filtrates and concentrate under reduced pressure to 200ml, add Sevag reagent (n-butanol: chloroform = 1:4, v / v) of 1 / 4 concentrated liquid volume, shake at 200r / min for 20min, centrifuge at 5000g for 10min, collect the supernatant, then deproteinize by Sevag method, repeat 12 Second-rate. The deproteinized extract was rotary ...

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Abstract

The invention discloses a low-molecular-weight blackberry polysaccharide and a preparation method thereof. The weight-average molecular weight of the low-molecular-weight blackberry polysaccharide is180-590 thousand Da; the particle size of the low-molecular-weight blackberry polysaccharide in solutions is 400-1,000 microns, and the dispersion index of the low-molecular-weight blackberry polysaccharide in the solutions is 0.18-0.29; the low-molecular-weight blackberry polysaccharide is prepared from, by weight, 9.8-11.2% of arabinose, 3.1-3.6% of galactose, 2.7-3.3% of glucose, 81.9%-83.5% ofgalacturonic acid and 0.40-0.42% of glucuronic acid; the IC50 value of scavenging activity on ABTS free radicals is 1.47-1.62 mg / mL, and the IC50 value of inhibitory activity on alpha-glucosidase is1.11-1.59 mg / mL. A water extract and alcohol precipitate method is adopted and combined with an ultrasonic degradation method to obtain the low-molecular-weight blackberry polysaccharide, and the low-molecular-weight polysaccharide has excellent oxidation resistance performance and hypoglycemic activity.

Description

technical field [0001] The invention relates to a polysaccharide, in particular to a low-molecular-weight blackberry polysaccharide and a preparation method thereof, belonging to the technical field of blackberry extracts. Background technique [0002] Diabetes is a chronic metabolic disease that can affect many organs, such as kidneys, eyes and nerves. According to the report of the World Health Organization, the death rate caused by diabetes will reach the seventh place in the world by 2030. Enzymes involved in glucose metabolism are often used as therapeutic targets for the development of new antidiabetic drugs. The most effective method is to slow down the formation of glucose by inhibiting the activity of α-glucosidase, thereby reducing the absorption of glucose and achieving the purpose of controlling postprandial blood sugar. [0003] At present, hypoglycemic drugs such as acarbose and miglitol used clinically control blood sugar by inhibiting the activity of α-gluc...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P39/06A61P43/00
CPCA61P39/06A61P43/00C08B37/0003C08B37/006
Inventor 扶雄窦祖满陈春
Owner 珠海中美普莱健康科技有限公司
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