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Method for coordinative extraction of auricularia heimuer melanin through coordination of cellulases and ultrasonic waves

A technology of cellulase and black fungus, which is applied in the direction of pharmaceutical formula, medical preparations containing active ingredients, anti-toxic agents, etc.

Active Publication Date: 2019-04-02
FUJIAN AGRI & FORESTRY UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

However, there is no research report on the application of cellulase in the extraction of black fungus melanin

Method used

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  • Method for coordinative extraction of auricularia heimuer melanin through coordination of cellulases and ultrasonic waves
  • Method for coordinative extraction of auricularia heimuer melanin through coordination of cellulases and ultrasonic waves
  • Method for coordinative extraction of auricularia heimuer melanin through coordination of cellulases and ultrasonic waves

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Experimental program
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Embodiment Construction

[0020] Materials and Methods

[0021] 1.1 Materials, Reagents and Instruments

[0022] 1.1.1 Material: black fungus fruiting body (purchased from RT-Mart Supermarket, Fuzhou City, Fujian Province).

[0023] Reagents and instruments: pure black fungus melanin. Reagents such as sodium hydroxide, sodium dihydrogen phosphate, citric acid, ferrous sulfate, and salicylic acid were of domestic analytical grade. Cellulase (Yuanye Bio), diphenylpicrylphenylhydrazine (DPPH) (Sigma Company), PS-G60 Jiekang ultrasonic cleaner, high-speed universal pulverizer (Tianjin Test Instrument Co., Ltd.), FE- 20 pH meter (Mettler Toledo Instrument Co., Ltd.), ST16R centrifuge (Thermo Company, USA), CP-214 electronic balance (Ohaus Instrument Co., Ltd.).

[0024] Determination of melanin content in extract

[0025] By scanning the ultraviolet total absorption spectrum of pure black fungus melanin, it is determined that the maximum absorption wavelength of black fungus melanin is at 215 nm ( fig...

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Abstract

The invention discloses a method for coordinative extraction of auricularia heimuer melanin through coordination of cellulases and ultrasonic waves. The preferable condition lies in that the additionquality of enzymes is 12mg, the enzymolysis temperature is 40 DEG C, the enzymolysis pH is 5.0, the enzymolysis time is 120min, the concentration of NaOH is 1.27mol / L, the ratio of materials to liquidis 1 to 40 g / ml, the ultrasonic power is 300W, the ultrasonic time is 52min, and the ultrasonic temperature is 60 DEG C. Under the best condition, the extraction yield of auricularia heimuer melanincan achieve 10.48%. Compared with the extraction yield of melanin of an ultrasonic wave group free from cellulases, arranged for experiment, the extraction yield of melanin is increased by 12.93%. Oxidation resisting results indicate that compared with the melanin extracted without the cellulases, the melanin extracted through coordination of the cellulases and the ultrasonic waves has the advantage that the capacity for clearing ABTS, DPPH and hydroxyl free radicals is higher. The method provides a foundation for establishment of application and development of efficient extraction and products of the auricularia heimuer melanin.

Description

technical field [0001] The invention relates to the technical field of melanin extraction, in particular to a method for synergistically extracting black fungus melanin by using cellulase and ultrasonic waves. Background technique [0002] Black Fungus( Auricularia heimuer F. Wu, B.K. Cui & Y.C. Dai), also known as cloud ear, light fungus, etc., belongs to Basidiomycota, Agaricaceae, Agaricaceae, and Auricularia genus. It is a common edible and medicinal fungus (Wu et al .2015; Wu Fang et al. 2015), which has rich nutritional value and pharmacological activity. Black fungus has a variety of active ingredients, such as melanin, protein, polysaccharides, amino acids, dietary fiber, calcium, iron, etc. (Luan Shuying 2016). Among them, melanin is one of the main active components of black fungus (Wu Zhichao et al. 2017), which plays an important role in its pharmacological activity. Natural melanin is a heterogeneous high-molecular-weight compound formed by oxidative polym...

Claims

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Application Information

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IPC IPC(8): A61K36/07A61P39/06
CPCA61K36/07A61K2236/15A61K2236/19A61K2236/331A61K2236/51A61K2236/53A61P39/06
Inventor 傅俊生侯若琳刘鑫吴小平郑明锋
Owner FUJIAN AGRI & FORESTRY UNIV
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