Method for preparing flaxseed gum oligosaccharides by hydrogen peroxide oxidation degradation technology

A linseed gum, oxidative degradation technology, applied in the field of biomedicine and functional food, can solve problems such as complex process, achieve the effect of economical raw material basis, simple and reliable preparation method, excellent physical and chemical properties and antioxidant capacity

Inactive Publication Date: 2017-07-25
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are individual documents related to hydrogen peroxide-assisted acid hydrolysis to degrade polysaccharides to prepare oligosaccharides (CN201310077612.6, a method for preparing konjac glucomannan oligosaccharides and the film evaporator used. The enzymatic method is simpler and more practical for raw materials), but the preparation of functional oligosaccharides by high-pressure assisted hydrogen peroxide oxidation degradation method has not been reported, especially the preparation of new oligosaccharides with antioxidant activity by using this method to degrade linseed gum to report

Method used

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  • Method for preparing flaxseed gum oligosaccharides by hydrogen peroxide oxidation degradation technology
  • Method for preparing flaxseed gum oligosaccharides by hydrogen peroxide oxidation degradation technology
  • Method for preparing flaxseed gum oligosaccharides by hydrogen peroxide oxidation degradation technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Preparation of linseed gum oligosaccharide

[0039] Prepare flaxseed gum oligosaccharides according to the following steps:

[0040] (1) Dissolution of linseed gum: take commercially available linseed gum and dissolve it in 0.1-0.6 mol / L hydrogen peroxide solution under dynamic and continuous stirring to fully dissolve the linseed gum. The addition amount of linseed gum is 2% ( w / v, the mass of linseed gum added in 100mL of hydrogen peroxide solution is 2g);

[0041] (2) Oxidative degradation of linseed glue: take the linseed glue obtained in step (1), divide 100 mL into 150 mL screw-top glass bottles, seal them, and degrade them in a high-pressure steam sterilizer to control the reaction Time 0.5-3.0h, reaction temperature 80℃-125℃, after the reaction, take it out and cool to room temperature;

[0042] (3) Decolorization: Suction filter the mixture liquid obtained in step (2) and repeatedly wash the filter cake, combine and collect the filtrate, using 1% (w / v, the ...

Embodiment 2

[0046] Example 2: Evaluation of the degradation effect of linseed gum

[0047] The degradation effect of linseed gum was evaluated according to the following method:

[0048] (1) Changes in the degradation rate of linseed gum:

[0049] After the reaction, take it out to cool, stop the reaction in an ice water bath for 10 minutes, filter with suction, and repeatedly wash the filter cake with distilled water, combine the filtrate, concentrate to 100mL, take 0.10mL, use DNS colorimetric method to measure reducing sugar content; take another 0.01mL, use phenol -Measure the total sugar content by the sulfuric acid method, and calculate the degradation effect of flaxseed gum according to the formula (1) based on the yield of the reducing terminal sugar group. figure 2 Shown.

[0050]

[0051] by figure 2 In a, it can be seen that when the temperature is lower than 110℃, the degradation degree of linseed gum is very small. When the temperature is higher than 110℃, the degradation rate of l...

Embodiment 3

[0061] Example 3: Evaluation of the separation and purification effect of linseed gum oligosaccharides (FGOS)

[0062] The degradation products were separated and purified according to the method described in Example 1, and the decolorization and ultrafiltration effects were evaluated according to formulas (2) and (3). The results are shown in Table 2.

[0063]

[0064]

[0065] Table 2 Decolorization and ultrafiltration results

[0066]

[0067] It can be seen from Table 2 that the decolorization rate can reach 86.77% by the method described in Example 1, and the obtained sugar liquid is ideally light yellow; the sugar retention rate is 97.21%, and there is almost no sugar loss. The theoretical average degree of polymerization of the product before and after ultrafiltration dropped from 5.02 to 4.65. From the SEC elution curve ( Image 6 ), after ultrafiltration, the large component broad peak (about 35-45min) disappeared, indicating that the ultrafiltration had a good role in retai...

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Abstract

The invention discloses a method for preparing flaxseed gum oligosaccharides by a hydrogen peroxide oxidation degradation technology. The flaxseed gum oligosaccharides (FGOSs) are prepared from commercial flaxseed gum by adopting a high pressure assisted hydrogen peroxide oxidation degradation technology through dynamic dissolving, oxidative degradation, suction filtering, decolorizing, ultrafiltration, dialysis, concentration and freeze-drying. The FGOSs obtained in the invention are a semisolid viscous substance, have a light reddish brown color and special fragrance, can be easily dissolved in water, are insoluble in ethanol and other organic solvents, can easily absorb moisture, are a typical carbohydrate substance, and contain uronic acid; the FGOSs have uniform components, the average molecular weight is 1047 Da, the FGOS have a pyranose ring structure, belong to pyranose, and are composed of the following seven monosaccharides: rhamnose, fucose, arabinose, xylose, mannose, galactose and glucose, and the FGOS have certain anti-oxidation ability, and have a high removal rate on hydroxyl free radicals, DPPH free radicals, ABTS free radicals and superoxide anion free radicals.

Description

Technical field [0001] The invention belongs to the field of biomedicine and functional food, and specifically relates to a method for preparing flaxseed gum oligosaccharides by using a hydrogen peroxide oxidative degradation method. Background technique [0002] Oligosaccharides, also known as oligosaccharides, refer to a low-molecular-weight carbohydrate formed by connecting 2-10 monosaccharide molecules through various specific glycosidic bonds. There are many kinds of oligosaccharides, which are widely present in animal, plant and microbial tissues or metabolites. According to whether they have physiological health care functions, they can be divided into ordinary oligosaccharides and functional oligosaccharides. Functional oligosaccharides have a series of special physiological functions such as value-added bifidobacteria, regulation of gastrointestinal function, prevention of maladies, protection of liver, anti-tumor, anti-aging, and enhancement of immunity. In recent years...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C07H3/04C07H3/06C07H1/00C07H1/06A61P39/06A23L33/125
CPCC08B37/0003A23V2002/00C07H1/00C07H1/06C07H3/04C07H3/06C08B37/006A23V2200/02A23V2250/28
Inventor 汪勇梁珊滕英来张宁仇超颖
Owner JINAN UNIVERSITY
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