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Lactobacillus-ginseng polysaccharide composition and its preparation method and use

A technology of ginseng polysaccharide and composition, applied in the field of lactic acid bacteria and ginseng polysaccharide composition, can solve problems such as liver damage and cancer, and achieve the effects of reducing MDA content and enhancing antioxidant enzyme activity

Active Publication Date: 2014-11-05
JILIN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most of the antioxidants currently used are synthetic and have the potential to cause liver damage or contribute to the development of cancer

Method used

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  • Lactobacillus-ginseng polysaccharide composition and its preparation method and use
  • Lactobacillus-ginseng polysaccharide composition and its preparation method and use
  • Lactobacillus-ginseng polysaccharide composition and its preparation method and use

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preparation example Construction

[0031] The preparation process of the ginseng neutral polysaccharide is as follows: Weigh 200 g of dried ginseng root powder (20 mesh), decoct in 2000 ml hot water twice, each time for 2 hours, filter the boiled solution with gauze (six layers), combine the filtrate and concentrate to 800ml, centrifuge to discard the precipitate, add 3 times the amount of absolute ethanol to the supernatant for ethanol precipitation, let it stand overnight at 4°C, centrifuge to discard the supernatant, and freeze-dry the precipitate to obtain ginseng total polysaccharides, which are treated with DEAE-cellulose ion Separation and purification by exchange chromatography column, eluting with distilled water and 0.5M NaCl solution successively, detecting polysaccharide content with sulfuric acid-phenol, combining water eluent to obtain neutral polysaccharide of ginseng;

[0032] Ginseng neutral polysaccharides were dialyzed with 0.5M NaCl solution eluent (molecular weight cut-off 3500Da) and freeze...

Embodiment 1

[0042] Mix 9 parts of ginseng acidic polysaccharide powder, 28 parts of skimmed milk powder, 18 parts of sucrose and 30 parts of dextrin, add absolute ethanol, and fully stir to form a soft material. pressure will disperse;

[0043] Granulate the prepared soft material, pass through a 20-mesh sieve to obtain wet granules, put the wet granules in an oven at 55°C, and dry them by blasting for (2-2.5) hours to reduce the moisture content to below 3%. Sieve through a 16-mesh sieve;

[0044] Then add 1.6 parts of mannitol, 0.8 parts of magnesium stearate, 0.8 parts of citric acid and 4 parts of Lactobacillus plantarum C88 starter into the dry granules, fully and evenly, to obtain the composition of lactic acid bacteria and ginseng polysaccharide.

Embodiment 2

[0046] Mix 11 parts of ginseng neutral polysaccharide powder, 32 parts of skimmed milk powder, 22 parts of sucrose and 32 parts of dextrin, add absolute ethanol, and fully stir to form a soft material. loosen with light pressure;

[0047] Granulate the prepared soft material, pass through a 20-mesh sieve to obtain wet granules, put the wet granules in an oven at 55°C, and dry them by blasting for (2-2.5) hours to reduce the moisture content to below 3%. Sieve through a 16-mesh sieve;

[0048] Then add 2.4 parts of mannitol, 1.2 parts of magnesium stearate, 1.2 parts of citric acid and 6 parts of Lactobacillus plantarum C88 starter into the dried granules, fully and evenly, to obtain the composition of lactic acid bacteria and ginseng polysaccharide.

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Abstract

The invention provides a lactobacillus-ginseng polysaccharide composition and its preparation method and use, and belongs to the technical field of lactobacillus compositions. The lactobacillus-ginseng polysaccharide composition comprises 4-6 parts of a lactobacillus plantarum C88 starter, 9-11 parts of ginseng extract, 28-32 parts of dried skim milk, 18-22 parts of cane sugar, 30-32 parts of dextrin, 1.6-2.4 parts of mannitol, 0.8-1.2 parts of magnesium stearate and 0.8-1.2 parts of citric acid. The lactobacillus-ginseng polysaccharide composition has good antioxidation performances which comprise removing DPPH free radical and ABTS free radical in vitro, improving vitality of antioxidant enzyme in naturally aging mouse serum and liver in an in-vivo test and reducing MDA content of naturally aging mouse serum and liver.

Description

technical field [0001] The invention belongs to the technical field of lactic acid bacteria compositions, and in particular relates to a composition of lactic acid bacteria and ginseng polysaccharide, a preparation method and an application. Background technique [0002] Reactive oxygen species (ROS) such as hydroxyl radicals, superoxide anion, and hydrogen peroxide are usually spontaneously produced and play important roles in the metabolism of living cells. However, excessive reactive oxygen species cause irreversible damage to many important biomacromolecules such as proteins, lipids and DNA. Oxidative damage in the body is closely related to aging and neurodegenerative diseases, such as cancer, cardiovascular disease, Parkinson's disease, Alzheimer's disease, etc. Supplementing with antioxidant substances can help delay or prevent the damage caused by oxidative stress. However, most of the antioxidants currently used are synthetic and have the potential to cause liver ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/74A23L1/09A61P39/06A61K31/715
Inventor 李盛钰赵玉娟牛春华张健段翠翠王晓慧
Owner JILIN ACAD OF AGRI SCI
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