Method for detecting antioxidative activity compound in mixture

A technology for antioxidant activity and compounds, applied in the field of chromatographic analysis technology and active compound detection, can solve the problems of low efficiency, poor stability and sensitivity, time-consuming, labor-intensive and material-consuming, and achieve low detection limit, stable quality and low cost Effect

Inactive Publication Date: 2012-06-20
NORTHWEST A & F UNIV
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Problems solved by technology

Therefore, a method with high sensitivity, good repeatability, easy operation and rapid detection is provided for the detection of antioxidant active compounds and antioxidants in the mixture, so as to eliminate the disadvantages of time-consuming, labor-intensive, material-consuming and low-efficiency of traditional methods, and similar methods are stable Problems with poor sex and sensitivity

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  • Method for detecting antioxidative activity compound in mixture

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Embodiment Construction

[0024] In order to further illustrate the present invention, an example is given below: Utilize the system that the present invention establishes to detect antioxidant active compounds in the 50% ethanol extract of Eucommia ulmoides plant:

[0025] 1 Sample and solvent treatment

[0026] All samples and solvents required for HPLC analysis were filtered (0.45 μm, RC 55; Schleicher & Schuell; Dassel, Germany). Take 10 g of eucommia leaf samples, add 100 mL of 50% ethanol, and extract under reflux at 80°C for 60 min, filter the extract and evaporate the solvent under reduced pressure to obtain the extract. The extract was prepared as a 10 mg / mL solution in 50% ethanol.

[0027] 2 Preparation method of free radical solution

[0028] ABTS stock solution preparation. 0.110g ABTS in 90mL phosphate buffer (NaCl 8.2g / L, KH 2 PO 4 0.26g / L, Na 2 HPO 4 1.4g / L, KCl 0.14g / L), add 10mL K 2 S 2 o 8 solution (0.8g K 2 S 2 o 8 dissolved in 1L of the aforementioned phosphate buffer...

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Abstract

The invention discloses a device for detecting an antioxidative activity compound in a mixture and an operation method of the device, and belongs to a chromatographic analysis technology and the technical field of detection of active compounds. Components with antioxidative activity in the mixture can be rapidly detected. The device consists of a high performance liquid chromatograph (HPLC) comprising a high-pressure pump, a liquid chromatographic column and an ultraviolet-visible detector, a high-pressure pump, a visible detector, a tee joint for the HPLC, a buffer and a reaction coil. The antioxidative activity components in the mixture are measured by chemical free radical ABTS. Each component in the mixture is subjected to liquid chromatographic separation and reacts with the free radical one by one, the free radical fades to form a negative peak through active ingredients, and the number of the antioxidative activity components and the retention time and the antioxidative activity of each component can be obtained by contrasting a chromatogram with a chromatogram after the reaction with the free radical. The method is rapid, simple, convenient and high in sensitivity.

Description

1. Technical field: [0001] The invention belongs to the field of chromatographic analysis technology and active compound detection technology, more specifically, it is a method for online detection of antioxidant active compounds in a mixture by using high performance liquid chromatography and a free radical detection system. 2. Background technology: [0002] High-performance liquid chromatography is an instrument and method for efficiently separating and analyzing compounds in a mixture. Equipped with different detectors, it can detect compounds of different properties in the mixture. Commonly used detectors are: ultraviolet absorption detector (UVD), diode array detector (PDAD), fluorescence detector (FLD), differential refractive index detector (RID), evaporative light scattering detector (ELSD), mass spectrometer ( MSD). These detectors detect compounds based on certain chemical and physical properties of the compounds, which are not closely related to the activity of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/88
Inventor 张强苏印泉李秀红朱铭强
Owner NORTHWEST A & F UNIV
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