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Lucid ganoderma laccase preparation and production method thereof

A production method and preparation technology, which are applied in the field of industrial enzyme preparations and their preparation, can solve the problems of pollution, increase the difficulty of post-processing of fermentation broth, etc.

Inactive Publication Date: 2007-08-01
ANHUI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0003] At present, there are many studies on the preparation of laccase by liquid submerged fermentation at home and abroad. During the preparation process, aromatic compounds or / and heavy metal ions need to be added as inducers, which will increase the difficulty of post-treatment of the fermentation broth and easily cause new pollution.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0029] (1) Preparation of primary culture medium

[0030] 1. Take 1 part of glucose (hereinafter referred to as A), 1 part of corn flour (hereinafter referred to as B), 0.3 parts of wheat bran (hereinafter referred to as C), make up to 100 parts with distilled water, stir well, and adjust the pH to 4. 5. Sterilize for later use.

[0031] 2. Take A9 parts, B9 parts, and C8 parts, make up to 100 parts with distilled water, adjust the pH to 5.5, and perform the same operation as Example 1.

[0032] 3. Take 1 part of A, 1 part of B, and 0.5 parts of C, make up to 100 parts with distilled water, adjust the pH to 4.8, and perform the same operation as Example 1.

[0033] 4. Take A7 parts, B7 parts, and C6 parts, make up to 100 parts with distilled water, adjust the pH to 5.2, and perform the same operation as Example 1.

[0034] 5. Take A1, B1, and C6 parts, make up to 100 parts with distilled water, adjust the pH to 5.0, and perform the same operation as Example 1.

[0035] 6. T...

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PUM

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Abstract

The invention relates to ganoderam lucidum karst laccase preparation, which in detail relates to a blue copperas- containing liquid laccase preparation produced with Ganoderma lucidum through inclined-plane culture and two- stage fermentation, the blue copperas concentration is 0.15- 0.40 mol / l. The culture medium of first- stage comprises amylaceum, maize flour, bran flour and water, and the culture condition is 25- 35 Deg. C and for 6- 12 days. The further fermentation culture medium comprises bran flour, peanut shell powder, maize flour and water, and the culture condition is 25- 35 Deg. C and for 3- 7 days. The enzymatic activity of said preparation is 32841.36U / l (taking ABTS as substrate). It can be used in field of textile, paper making, food and beverage after being diluted for 200- 250 times, and used for biological sensor production.

Description

1. Technical field [0001] The invention relates to an industrial enzyme preparation and a preparation method thereof, in particular to a ganoderma laccase preparation and a production method thereof. 2. Background technology [0002] Laccase is a copper-containing polyphenol oxidase, which is ubiquitous in white rot fungi and also produced in a few lower fungi and plants, mostly secreted glycoproteins. Laccase has a wide range of potential applications, such as the removal of lignin in the pulp and paper industry, the biological elimination of xenobiotic compounds can effectively remove lignin in pulping wastewater, and the benefits of water purification are obvious; In biological bleaching, in the fungal system that degrades lignin in pulp, laccase, lignin peroxidase and manganese peroxidase are the three main enzymes that play a major role. Laccase has its own irreplaceable advantages: laccase It is more stable and can function more effectively after immobilization; it do...

Claims

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Application Information

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IPC IPC(8): C12N9/02
Inventor 王林肖亚中洪宇植房伟
Owner ANHUI UNIVERSITY
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