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Calibration reagent and preparation method for indican test

The technology of a calibration solution and indigoside is applied in the field of calibration solution and preparation for uridine indigo test, which can solve the problems of inaccuracy and the like, and achieve the effect of good precision.

Active Publication Date: 2008-07-02
北京迈达康医疗设备制造有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the determination of uroindoside is not very accurate because the colorimetric method is used to estimate the level of uroindoside with the naked eye according to the depth of the color. The absorbance detection method we invented improves the accuracy of uroindoside determination.

Method used

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  • Calibration reagent and preparation method for indican test
  • Calibration reagent and preparation method for indican test
  • Calibration reagent and preparation method for indican test

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1: the buffer solution of indoside calibrator

[0046] Take 11.5 g of disodium hydrogen phosphate, 2 g of potassium dihydrogen phosphate, 80 g of sodium chloride, and 2 g of potassium chloride, and dissolve them in 1000 mL of deionized water to make 10×PBS buffer solution.

Embodiment 2

[0047] Embodiment 2: the buffer solution of indoside calibrator

[0048] Take 10 g of disodium hydrogen phosphate, 3 g of potassium dihydrogen phosphate, 70 g of sodium chloride, and 3 g of potassium chloride, and dissolve them in 1000 mL of deionized water to make 10×PBS buffer solution.

Embodiment 3

[0049] Embodiment 3: the buffer solution of indoside calibrator

[0050] Take 12 g of disodium hydrogen phosphate, 1 g of potassium dihydrogen phosphate, 85 g of sodium chloride, and 4 g of potassium chloride, and dissolve them in 1000 mL of deionized water to make 10×PBS buffer solution.

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Abstract

The invention discloses indoxyl-Beta-glucoside calibration solution, which comprises (by weight parts) disodium hydrogen phosphate 9-12, potassium dihydrogen phosphate 1-4, sodium chloride 70-90, and potassium chloride 1-4. The experimental observation and clinical applications indicate that the inventive calibration solution has similar performance and test effect as the imported reagent and can replace the imported reagent for clinical applications.

Description

technical field [0001] The invention relates to a detection reagent calibration solution and a preparation method, in particular to a calibration solution for uroindigoside testing and a preparation method. Background technique [0002] The level of indigoside in urine reflects the level of indole, a by-product produced by intestinal bacteria in the body. The level of indigoside also reflects the digestion of protein in the body. The spoilage of undigested and absorbed protein in food under the action of intestinal bacteria will produce many by-products, among which indole indo1e is one of the main by-products. Indole is produced by splitting tryptophan. After absorption, it flows to the liver along with the blood, where it is transformed into indoxyl. Indoxyl is coupled to potassium sulfate or glucuronic acid to form indoxyl, which then flows through the blood through the kidneys. kidney into the urine. [0003] In order to better use this detection item in clinic and fu...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N21/25
Inventor 孙允高朱礼华马景东
Owner 北京迈达康医疗设备制造有限公司
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