Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Electrochemical method for rapidly detecting plasma indoxyl sulfate

A technology of indoxyl sulfate and a detection method, applied in the field of electrochemical detection, to achieve the effects of simple production process, avoiding energy loss, and avoiding experimental cross-contamination

Pending Publication Date: 2021-11-26
重庆医科大学国际体外诊断研究院
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, although there have been reports on the detection of plasma indoxyl sulfate by electrochemical methods at home and abroad, there is no electrochemical method for the quantitative determination of plasma indoxyl sulfate using SPCE as the electrode material and differential pulse voltammetry (DPV) as the detection method. Research reports on chemical methods

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Electrochemical method for rapidly detecting plasma indoxyl sulfate
  • Electrochemical method for rapidly detecting plasma indoxyl sulfate
  • Electrochemical method for rapidly detecting plasma indoxyl sulfate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: the preparation of screen printing carbon electrode of the present invention

[0035] The screen printing carbon electrode of the present invention is to sequentially print carbon paste, Ag / AgCl paste and insulating paste on a PET substrate. Specifically include the following steps:

[0036] 1) Clean the PET substrate, print carbon paste on the PET substrate after drying, make carbon working electrode and carbon paste auxiliary electrode, and dry at room temperature;

[0037] 2) printing silver paste containing silver chloride on the above-mentioned PET substrate to make Ag / AgCl reference electrode, and drying at normal temperature;

[0038] 3) Avoid the circular working area, and print insulating paste on the above-mentioned PET substrate to cover the wire;

[0039] 4) The above-mentioned working electrode, auxiliary electrode and reference electrode form a circular working area, and each electrode is connected to the interface through a wire under the ...

Embodiment 2

[0041] Embodiment 2: a kind of electrochemical method for rapidly detecting plasma indoxyl sulfate described in this embodiment, specifically comprises the following steps:

[0042] 1) Add 300 μL of plasma and 900 μL of acetonitrile into 2 mL Ep tubes, vortex for 1 min, let stand for 10 min, then centrifuge at 13300 g for 10 min at 4 °C, and take the supernatant;

[0043] 2) Drying the supernatant obtained in step 1) with nitrogen at 45°C;

[0044] 3) Reconstitute the sample obtained in step 2) with 300 μL of 0.10 mol / L PBS solution at pH 2.0, and vortex for 90 seconds to obtain a reaction mixture;

[0045] 4) Take 50 μL of the reaction mixture obtained in step 3) and drop it on the SPCE. The SPCE is connected to an electrochemical workstation, and DPV technology is used for electrochemical detection. Its parameters are set as follows: low potential 0.1V, high potential 1.0V, pulse width 150mV, pulse width 0.05s, pulse period 0.6s.

[0046] Preferably, the PBS solution is pr...

Embodiment 3

[0048] This embodiment is to investigate the electrochemical behavior of indoxyl sulfate. Using CV to characterize the electrochemical behavior of indoxyl sulfate on SPCE, the detection solution is a PBS solution of 0.10mol / LpH 2.0, and the sweep speed is 50mV / s. The results are as follows figure 2 As shown in A. At 40.0μmol / L indoxyl sulfate, two irreversible oxidation peaks Ⅰ and Ⅱ appeared at 0.485V and 0.862V respectively, and there was no reduction peak (dashed line b). Since the peak potential of oxidation peak I is lower than that of oxidation peak II, low oxidation potential is beneficial to avoid noise interference during the detection process, and the peak current of oxidation peak I is higher than that of oxidation peak II. Considering comprehensively, the peak current value of oxidation peak Ⅰ was used for quantitative analysis of indoxyl sulfate. Further use DPV to characterize the electrochemical behavior of indoxyl sulfate, the detection solution is 0.10mol / L P...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an electrochemical method for rapidly detecting plasma indoxyl sulfate. According to the method, the modification-free silk-screen printing carbon electrode is adopted, the differential pulse voltammetry can effectively reduce the background current, electrochemical detection is carried out by adopting the differential pulse voltammetry, and according to the linear relation between an electrochemical response signal and the concentration of a to-be-detected substance, rapid quantitative detection of the indoxyl sulfate in the plasma is achieved. Instruments and equipment used in the method are small, the screen-printed carbon electrode has the advantages of simple design, low price, disposable use, less sample consumption, miniaturization and the like, the electrochemical detection method is simple, convenient and rapid, has good repeatability, stability and accuracy, can realize rapid quantitative detection of indoxyl sulfate in plasma, the method is helpful to understand the metabolic condition of the indoxyl sulfate in gestational diabetes mellitus, and provides laboratory basis for the occurrence mechanism, diagnosis, treatment and prognosis of the disease and complications thereof.

Description

technical field [0001] The invention belongs to the field of electrochemical detection, and mainly relates to an electrochemical method for rapidly detecting plasma indoxyl sulfate. Background technique [0002] Gestational diabetes mellitus (GDM) is the most common pregnancy complication, which refers to diabetes or impaired glucose tolerance that is discovered or occurs for the first time during pregnancy. GDM seriously affects the health of mother and fetus. Women with GDM are prone to multiple complications, including gestational hypertension, cardiovascular disease, and preeclampsia, and their offspring are at increased risk of complications, including macrosomia, preterm birth, shoulder dystocia, neonatal hypoglycemia, and neonatal Admission, etc. In addition, the recurrence rate of GDM is as high as 48%, and 20% to 50% of women with GDM are diagnosed with type 2 diabetes within 10 years. However, the pathogenesis of GDM has not been clarified, and GDM not only cause...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N27/327G01N27/48
CPCG01N27/3277G01N27/48
Inventor 张晓清朱阳丁敏蒋梦丹李彦茹
Owner 重庆医科大学国际体外诊断研究院
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com