Effective heat treatment method for identifying and quarantine fruit flies and application thereof

A technology for fruit flies and detection results, applied in the field of bioinformatics, which can solve problems such as lack of clear standards

Pending Publication Date: 2022-07-12
CHINESE ACAD OF INSPECTION & QUARANTINE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are no clear standards for heat tre

Method used

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  • Effective heat treatment method for identifying and quarantine fruit flies and application thereof
  • Effective heat treatment method for identifying and quarantine fruit flies and application thereof
  • Effective heat treatment method for identifying and quarantine fruit flies and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Heat treatment of quarantine fruit fly larvae

[0029] Put the 3rd instar larvae of fruit fly into a glass bottle containing moisturizing filter paper, seal it and put it in a water bath at 38°C for 5h. The larvae of the control group were naked worms and were placed in a rearing environment at 25°C. Then, the larvae of the heat treatment group and the control group were quick-frozen with liquid nitrogen and stored in a -80°C refrigerator.

[0030] 2. Sample metabolome preprocessing

[0031] Weigh 50 mg of sample, add 1000 μL of extraction solution (methanol: acetonitrile: water = 2: 2: 1 (V / V), containing isotope-labeled internal standard mixture);

[0032] 35Hz grinding treatment 4min, ultrasonic 5min (ice water bath);

[0033] Repeat the grinding-sonication step 2 to 3 times;

[0034] -40℃ for 1h;

[0035] Centrifuge the sample at 4°C, 12000rpm (centrifugal force 13800(×g), radius 8.6cm) for 15min;

[0036] Take the supernatant and put it in the sample bottl...

Embodiment 2

[0060] Heat treatment of fruit fly larvae: Put the third instar larvae of fruit fly into a glass bottle containing moisturizing filter paper, seal it and put it in a water bath at 38°C for 1, 2, 3, and 5 hours, respectively. The larvae of the control group were naked worms and were placed in a rearing environment at 25°C. Then, the larvae of the heat-treated group and the control group were quick-frozen with liquid nitrogen and stored in a -80°C refrigerator. After determining the contents of tyrosine and 3-hydroxykynurenine, respectively, t-test and Log 2 Fold-fold analysis. The data analysis results of each group are shown in the following table:

[0061] Table 3 Analysis results of two biomarkers under different heat treatment time conditions

[0062]

[0063]

[0064] The experimental results show that only after 38 ℃ hot water treatment for 5h, the analysis results of tyrosine and 3-hydroxykynurenine satisfy P2 The folding factor is greater than 1, which proves t...

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PUM

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Abstract

The invention relates to the technical field of bioinformatics, in particular to an effective heat treatment method for identifying and quarantine fruit flies and application thereof. The method comprises the following steps: identifying quarantine fruit flies and non-quarantine fruit flies according to the difference of the content of metabolites; the metabolite comprises tyrosine, 3-hydroxykynurenine, 4-hydroxyhippuric acid, adenylic acid, dopa, trehalose, malic acid, homoserine, glucose, fumaric acid, yellow uric acid, mannitol, succinic acid, inositol, threonic acid, pinocembriose, uric acid, methionine, serine, oleic acid, stearic acid, palmitoleic acid, glycolic acid, glycine, palmitic acid, putrescine, lactic acid and aminomalonic acid. The compound is one or more of beta-hydroxybutyric acid, alpha-hydroxyglutaric acid, sedoheptulose-7-phosphoric acid or adenosine. The invention provides a plurality of markers which can be used for identifying and quarantine the fruit flies and can effectively distinguish whether the fruit flies are subjected to heat treatment or not.

Description

technical field [0001] The invention relates to the technical field of bioinformatics, in particular to a method and application of an effective heat treatment method for identifying and quarantine fruit flies. Background technique [0002] Up to now, heat treatment technology has gradually attracted people's attention because of its environmental protection, no residue, and high safety. Heat treatment technology also began a new round of vigorous development. Heat treatment techniques include hot water, steam heat, forced hot air and dry heat treatment techniques. These techniques are also gradually applied to quarantine treatment. However, there is no clear standard for the heat treatment of guava fruit fly, South Asian fruit fly, mealybug and so on. [0003] Therefore, quickly judging whether the larvae have undergone quarantine treatment to better prevent the invasion of foreign harmful organisms has important theoretical significance and practical value for ensuring ...

Claims

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Application Information

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IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8813Y02A50/30
Inventor 刘波任荔荔薛美玲孔维恒邱烨刘鑫常亮王强张百怡齐玮
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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