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Method for simultaneously detecting concentrations of glycollic acid and hippuric acid in human urine

A technology of glycolic acid and hippuric acid, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as difficult to achieve rapid and stable detection, and achieve the effects of sensitive detection, fast analysis, and simple operation

Active Publication Date: 2020-03-27
THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV (GUANGZHOU RESPIRATORY CENT)
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Problems solved by technology

[0003] In order for the above-mentioned research results to be practically applied and fully play a role in the prevention and intervention of kidney stones, the inventors have detected any of the above-mentioned substances according to the prior art, and it is difficult to achieve rapid and stable results using the same method. to detect the concentration of two substances of any of the above groups

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  • Method for simultaneously detecting concentrations of glycollic acid and hippuric acid in human urine
  • Method for simultaneously detecting concentrations of glycollic acid and hippuric acid in human urine
  • Method for simultaneously detecting concentrations of glycollic acid and hippuric acid in human urine

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Embodiment

[0024] A method for simultaneously detecting the concentration of glycolic acid and hippuric acid in human urine, comprising the steps of:

[0025] S01, sample collection: collect the morning urine of patients with kidney stones in our hospital and the normal control population, and prevent pollution during sampling;

[0026] S02, sample preparation: take 5ml of water, 10ml of methanol, and 5ml of water to infiltrate, rinse and activate the C18 solid phase extraction column; dilute the urine collected by S01 5 times with deionized water and add it to the C18 solid phase In the extraction column, use the purified urine obtained by elution;

[0027] S03. Perform ion chromatography-conductivity analysis on the sample: filter the urine purified solution obtained in step S02 with a membrane filter and inject it into an ion chromatograph, and analyze the horses in the urine purified solution according to the following chromatographic conditions. The concentration of uric acid and g...

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Abstract

The invention relates to a method for simultaneously detecting concentrations of glycollic acid and hippuric acid in human urine. The method comprises: collecting a sample, preparing the sample, drawing a standard curve, performing ion chromatography-conductivity analysis on the sample, calculating the concentration of glycollic acid or hippuric acid in human urine and the like. According to the method, a solid-phase extraction method is adopted to separate and purify hippuric acid and glycollic acid in urine, and an ion chromatography-conductivity detection method is adopted to determine theconcentrations of the glycollic acid and the glycollic acid in actual urine. The method can simultaneously detect the concentrations of glycollic acid and hippuric acid in human urine, is sensitive indetection, rapid in analysis, good in repeatability, simple in operation, accurate and reliable, and can provide a good method reference for analysis and detection of hippuric acid and glycollic acidin actual urine.

Description

technical field [0001] The invention relates to the technical field of biochemical detection and analysis, in particular to a method for simultaneously detecting the concentrations of glycolic acid and hippuric acid in human urine. Background technique [0002] Kidney stones are a common and frequently-occurring disease in my country. If a marker that can predict high-risk groups for kidney stones is found, early intervention in their diet structure and lifestyle can reduce the suffering of patients and the economic burden of the government. The inventor compared the differences between urinary metabolites between patients with kidney stones and normal people by means of metabolomics (for details, see: H NMR-based metabolomic study of metabolic profiling for the urine of kidney stone patients), and found that 15 Through the ROC curve and the Logistic regression model, it was found that the accurate combination of "hippuric acid and histidine", "hippuric acid and glycine", "h...

Claims

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Application Information

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IPC IPC(8): G01N30/96G01N30/64G01N30/86
CPCG01N30/96G01N30/64G01N30/8634
Inventor 曾国华温耀安欧莉莉
Owner THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV (GUANGZHOU RESPIRATORY CENT)
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