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A method for simultaneously detecting the concentrations of glycolic acid and hippuric acid in human urine for non-disease diagnosis purposes

A technology of disease diagnosis and glycolic acid, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as difficult to achieve rapid and stable detection, and achieve the effects of sensitive detection, accurate and reliable methods, and simple operation

Active Publication Date: 2022-07-15
THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV (GUANGZHOU RESPIRATORY CENT)
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Problems solved by technology

[0003] In order for the above-mentioned research results to be practically applied and fully play a role in the prevention and intervention of kidney stones, the inventors have detected any of the above-mentioned substances according to the prior art, and it is difficult to achieve rapid and stable results using the same method. to detect the concentration of two substances of any of the above groups

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  • A method for simultaneously detecting the concentrations of glycolic acid and hippuric acid in human urine for non-disease diagnosis purposes
  • A method for simultaneously detecting the concentrations of glycolic acid and hippuric acid in human urine for non-disease diagnosis purposes
  • A method for simultaneously detecting the concentrations of glycolic acid and hippuric acid in human urine for non-disease diagnosis purposes

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Embodiment

[0024] A method for simultaneously detecting the concentrations of glycolic acid and hippuric acid in human urine for non-disease diagnosis purposes, comprising the steps of:

[0025] S01, sample collection: collect the morning urine of nephrolithiasis patients and normal control population in our hospital, and prevent pollution during sampling;

[0026] S02. Sample preparation: take 5ml of water, 10ml of methanol, and 5ml of water to infiltrate, rinse and activate the C18 solid phase extraction cartridge; dilute the urine collected in S01 by 5 times with deionized water and add the C18 solid phase In the extraction column, the purified urine is obtained by elution;

[0027] S03, the sample is analyzed by ion chromatography-conductivity method: the urine purified solution obtained in step S02 is filtered with a membrane filter and injected into the ion chromatograph, and the urine purified solution in the urine purified solution is analyzed according to the following chromatog...

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Abstract

The invention relates to a method for simultaneously detecting the concentrations of glycolic acid and hippuric acid in human urine for non-disease diagnosis purposes. The method includes: sample collection, sample preparation, standard curve drawing, and ion chromatography-conductivity analysis and calculation on the sample. Steps such as the concentration of glycolic acid or hippuric acid in human urine. The method adopts solid-phase extraction method to separate and purify hippuric acid and glycolic acid in urine, and adopts ion chromatography-conductivity detection method to determine the concentrations of glycolic acid and hippuric acid in actual urine. The method can simultaneously detect the concentrations of glycolic acid and hippuric acid in human urine, and has the advantages of sensitive detection, rapid analysis, good repeatability, simple operation, accurate and reliable method, and can provide analysis and detection of hippuric acid and glycolic acid in actual urine. A good method reference.

Description

technical field [0001] The invention relates to the technical field of biochemical detection and analysis, in particular to a method for simultaneously detecting the concentrations of glycolic acid and hippuric acid in human urine for non-disease diagnosis purposes. Background technique [0002] Kidney stones are a common and frequently-occurring disease in my country. If a marker that can predict the high-risk group of kidney stones is found, interventions on their diet structure and lifestyle in advance can reduce the suffering of patients and the economic burden of the government. In the early stage, the inventors compared the differences in urinary metabolites between kidney stone patients and normal people by means of metabolomics (for details, see: H NMR-based metabolomic study of metabolic profiling for the urine ofkidney stone patients), and found that 15 These differential metabolites were further analyzed by ROC curve and Logistic regression model. Compared with t...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/96G01N30/64G01N30/86
CPCG01N30/96G01N30/64G01N30/8634
Inventor 曾国华温耀安欧莉莉
Owner THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV (GUANGZHOU RESPIRATORY CENT)
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