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Healthy aging diagnosis marker and application

A technology for diagnosing markers and markers, applied in the field of biomedicine, to achieve the effects of high accuracy and sensitivity, high sensitivity and high accuracy

Inactive Publication Date: 2020-07-07
KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no domestic and international reports on the above-mentioned markers that can indicate healthy aging in real time

Method used

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  • Healthy aging diagnosis marker and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Diagnostic markers for healthy aging

[0041] A new blood healthy aging marker, which can be used alone or in combination of two or more to assess whether the elderly have the characteristics of healthy aging, including α-N-phenylacetyl-L-glutamine (Alpha- N-Phenylacetyl-L-glutamine), 4-Methylphenol, N6-methyladenosine, N1-methyl-2-pyridone-5-carboxamide (N1-Methyl -2-pyridone-5-carboxamide), 3-phenyllactic acid (DL-3-Phenyllactic acid), 5-Hydroxyindoleacetic acid (5-Hydroxyindoleacetate), Dopamine (Dopamine), 4-Hydroxyphenylpyruvate (4 -Hydroxyphenylpyruvate, Propionylglycine, 4-Aminohippuric acid, Indoxyl sulfate, 3,4-Dihydroxybutyric acid (2-Deoxytetronic acid), O-acetylserine ( O-Acetyl-L-serine), N2, N2-Dimethylguanosine (N2, N2-Dimethylguanosine), N-Tigloylglycine, Threitol, Acetic acid , methoxy-, Indole-3-pyruvic acid, L-Histidine, Homoarginine, Arachidonic Acid.

[0042] Among the 21 diagnostic markers for healthy aging, the ones that are significantly ...

Embodiment 2

[0049] Alpha-N-Phenylacetyl-L-glutamine (Alpha-N-Phenylacetyl-L-glutamine) was used as a single marker for the diagnosis of healthy aging. The specific implementation method is as follows:

[0050] 1. Obtain the subject sample and perform pre-processing: After collecting the subject's blood sample according to a standardized and reasonable operating procedure, add 100 μL of blood to 350 μL of methanol, vortex and mix, centrifuge at high speed, and take the supernatant. Concentrate and dry, add 100μL of acetonitrile for reconstitution, and pass through the filter plug to directly perform liquid chromatography-quadrupole-time-of-flight mass spectrometry (UHPLC-QTOFMS) testing.

[0051] 2. Data processing: After UHPLC-QTOFMS test, collect UHPLC-QTOFMS data for analysis. XCMS program is used for data processing, and the parameter setting refers to the software description. After the XCMS program is processed, the charge-to-mass ratio, retention time, peak area, and secondary mass spe...

Embodiment 3

[0054] Example 3 N6-Methyladenosine is a single marker for healthy aging diagnosis:

[0055] The determination of the subject's N6-methyladenosine is the same as steps 1-3 in Example 2. Compare the measured concentration of 5-hydroxyindoleacetic acid in the blood of the subject with the threshold of the general population (410 control groups). If the value exceeds the threshold of the general population, the subject has a higher healthy age. may. Among them, the blood concentration threshold of N6-methyladenosine in the general population is 0.11umol / L; the results show that the area under the characteristic curve line is 0.905, and the concentration of N6-methyladenosine in the blood of subjects is 0.18umol / L. L. This value is higher than the threshold for the general population (0.11umol / L), indicating that the individual has better healthy aging characteristics and should maintain its current status.

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Abstract

The invention discloses a healthy aging diagnosis marker and application thereof. The marker is prepared from one or more of alpha-N-phenylacetyl-L-glutamine, 4-methylphenol, N6-methyladenosine, N1-methyl-2-pyridone-5-carboxamide, 3-phenyllactic acid, 5-hydroxyindoleacetic acid, dopamine, 4-hydroxyphenylpyruvic acid, propionylglycine, 4-aminohippuric acid, indoxyl sulfate, 3,4-dihydroxybutyric acid, O-acetylserine, N2,N2-dimethyl guanosine, N-ticagrelor glycine, threitol, methoxyacetic acid, indole-3-pyruvic acid, L-histidine, homoarginine and arachidonic acid. The marker has the characteristics of high accuracy and high sensitivity, and can be directly used as an index for healthy aging.

Description

Technical field [0001] The invention belongs to the field of biomedical technology, and specifically relates to a diagnostic marker for healthy aging and its application. Background technique [0002] The aging rate of my country's population is more than twice that of Western countries. Among them, the number of people over 65 will increase from 119 million in 2010 to 360 million in 2050 (accounting for 25.6% of the total population). The intensification of the aging process and the high incidence of senile diseases have seriously affected the quality of life of Chinese people. Considering that senile diseases are often difficult to cure after they occur, it is very necessary to discover the key markers of healthy aging and to evaluate the healthy aging characteristics of the aging population. [0003] At present, the internationally recognized and known factors of healthy aging mainly focus on the discovery of longevity genes, such as the longevity genes FOXO3 and APOE. However...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/86G01N33/68G01N33/50
CPCG01N30/02G01N30/8675G01N33/5002G01N33/68G01N33/9413
Inventor 孔庆鹏李功华杨利琴
Owner KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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