Applications of set of amino acid marker in acute kidney injurydiagnostic kit

A technology for acute kidney injury and diagnostic reagents, which is applied in the field of application of three amino acid markers in acute kidney injury diagnostic kits, can solve the problems of cumbersome operation process, long detection time, and complicated detection methods, and achieve high sensitivity, The results are accurate and reliable

Inactive Publication Date: 2018-12-07
SECOND AFFILIATED HOSPITAL SECOND MILITARY MEDICAL UNIV
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the early warning markers of AKI discovered so far are specific proteins expressed and secreted when renal tubules are injured, among which neutrophil gelatinase-associated apolipoprotein (NGAL) is reported more, and it shows relatively high expression in experimental animals and clinical patients. It has a good diagnostic effect and is earlier than the traditional marker creatinine, but its detection method is more complicated than that of small molecule markers such as creatinine, and enzyme-linked immunoassay is mostly used for detection
This method has high sensitivity and specificity, but the operation process is cumbersome and the detection time is long, and the metabolites in the matrix may cross-react with the antibody and interfere with the determination results

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Applications of set of amino acid marker in acute kidney injurydiagnostic kit
  • Applications of set of amino acid marker in acute kidney injurydiagnostic kit
  • Applications of set of amino acid marker in acute kidney injurydiagnostic kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Screening of serum differential amino acids between renal ischemia-reperfusion rats and sham-operated rats

[0021] 1. Objects and methods

[0022] 1. Animal model preparation and grouping

[0023] Male Sprague-Dawley (SD) rats, weighing 180-200 g, were provided by the Experimental Animal Center of Second Military Medical University. Rats were bred for one week before experiments to adapt to the environment. The room temperature of the animals was about 25°C, and the relative humidity was about 60%. During the feeding period, the rats were free to eat and drink, and kept light and dark for 12 hours each day and night. The rats were fasted for 8 hours before the experiment. . Animals were randomly divided into control group and AKI group, with 30 animals in each group. Blood was collected through the retro-orbital venous plexus at 0, 2, 4, 6, 10, and 24 hours after reperfusion, and serum samples were separated after coagulation at room temperature. Rats in ...

Embodiment 2

[0044] Example 2: Constructing a ROC curve to compare the ability of 3 serum differential amino acids to diagnose acute kidney injury

[0045] Receiver operating curve (ROC) was used to evaluate the diagnostic ability of the above-mentioned differential amino acids, and compared with creatinine, blood urea nitrogen and NGAL. The results of the above three differential amino acids, traditional renal function markers creatinine and blood urea nitrogen, and the early diagnostic marker NGAL alone in the diagnosis of AKI are shown in Table 1. The results showed that the three amino acid markers of kynurenine, hippuric acid and oxyproline alone had a strong ability to diagnose acute kidney injury, and the areas under the ROC curve (AUC) were all greater than 0.7, which had clinical diagnostic significance. The three amino acid markers as well as the levels of creatinine and NGAL showed good diagnostic ability for AKI starting 1 hour after kidney injury, and the diagnostic ability fr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the field of disease diagnostic kit, and more specifically relates to applications of a set of amino acid markers in preparation of an acute kidney injury (AKI) diagnostic agent or a kit. The amino acid marker is one or a composition of two selected from kynurenine, hippuric acid, and pidolic acid. The AUC values of the three amino acid markers in independent diagnosis ofacute kidney injury at acute renal injury early stage diagnosis are all larger than 0.7, the diagnosis distinguish effect is excellent. The diagnosis capacities of kynurenine and hippuric acid are better than that of clinically used renal function marker creatinine and early stage diagnosis marker NGAL; the diagnosis time of the above three amino acids on AKI is earlier than that of clinically used renal function marker urea nitrogen. The invention also provides a targeting amino acid metabonomic detection method capable of realizing quantitative analysis of a plurality of amino acids at the same time, the sensitivity is high, and the results are accurate and reliable.

Description

technical field [0001] The invention relates to the field of disease diagnosis kits, in particular, the application of three amino acid markers in acute kidney injury diagnosis kits. Background technique [0002] Acute kidney injury (AKI) is a disease state in which the structure and function of the kidneys are damaged. It is characterized by sudden and continuous decline in renal function. It is a clinical syndrome caused by different etiologies and involving symptoms of various systems throughout the body. Mild renal injury can recover spontaneously after a period of time, and even if AKI patients are significantly relieved, their long-term cardiovascular risk will still increase. Patients with severe AKI must receive dialysis when the damage progresses to renal failure. In 2013, about 2.9 million hospitalized patients in my country suffered from AKI, and the mortality rate was about 24%. The occurrence of AKI increases the medical expenses and mortality of inpatients, a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 张凤陶霞陈万生朱桑夏天一翟健秀龚晓斌高守红王清华
Owner SECOND AFFILIATED HOSPITAL SECOND MILITARY MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap