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Method for the detection of benzene, toluene and xylene metabolites in urine

A detection method and metabolite technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as the inability to fully detect metabolites of benzene compounds, achieve the effect of reducing matrix interference and improving measurement accuracy

Active Publication Date: 2020-10-02
THE BEIJING PREVENTION & TREATMENT HOSPITAL OF OCCUPATIONAL DISEASE FOR CHEM IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above method uses solid phase extraction to separate metabolites. Due to the limitation of extraction conditions, only a limited number of four metabolites can be detected, so it is impossible to comprehensively detect metabolites of benzene compounds.

Method used

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  • Method for the detection of benzene, toluene and xylene metabolites in urine
  • Method for the detection of benzene, toluene and xylene metabolites in urine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Sample processing:

[0069] Put 300 μL of urine in a centrifuge tube, add 900 μL of methanol, vortex and shake evenly, and centrifuge at 15000 rpm for 15 minutes to obtain the supernatant. Put 500 μL of the supernatant in a new centrifuge tube, and add 2 mL of ultrapure water, vortex and shake evenly to obtain the first sample solution. Place 1 mL of the first sample solution in a 2 mL sample bottle and inject the sample to analyze the content of S-PMA, 8-OHdG, 2-MHA, 3-MHA, and 4-MHA.

[0070] Put 20 μL of the first sample solution and 1980 μL of the 15 vol% methanol aqueous solution in a new centrifuge tube, vortex and shake evenly to obtain the second sample solution. Place 1 mL of the second sample solution in a 2 mL sample bottle and inject the sample to analyze the content of HA.

[0071] Standard solution preparation:

[0072] Using 15 vol% methanol in water as the solvent, mixed standard solutions of different concentrations were prepared. The concentration range of ...

Embodiment 2

[0087] Sample processing:

[0088] Put 300 μL of urine in a centrifuge tube, add 900 μL of methanol, vortex and shake evenly, and centrifuge at 15000 rpm for 15 minutes to obtain the supernatant. Put 500 μL of the supernatant in a new centrifuge tube, and add 2 mL of ultrapure water, vortex and shake evenly to obtain the first sample solution. Place 1 mL of the first sample solution in a 2 mL sample bottle and inject the sample to analyze the content of S-PMA, t,t-MA, 8-OHdG, 2-MHA, 3-MHA, and 4-MHA.

[0089] Put 20 μL of the first sample solution and 1980 μL of the 15 vol% methanol aqueous solution in a new centrifuge tube, vortex and shake evenly to obtain the second sample solution. Place 1 mL of the second sample solution in a 2 mL sample bottle and inject the sample to analyze the content of HA.

[0090] Standard solution preparation:

[0091] Using 15 vol% methanol in water as the solvent, mixed standard solutions of different concentrations were prepared. 8-OHdG concentratio...

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Abstract

The invention discloses a method for detecting benzene, methylbenzene and xylene metabolins in urine. The method comprises the following steps: (1) mixing urine with a first solvent, centrifuging so as to obtain supernate, mixing the supernate with a second solvent so as to obtain a first sample liquid, and detecting a first generation of metabolin in the first sample liquid through a liquid chromatogram tandem mass spectrum, wherein the first generation of metabolin comprises phenylmercapturic acid acid S-PMA, 8-hydroxy-2-deoxyguanosine 8-OHdG, 2-methyl hippuric acid 2-MHA, 3-methyl hippuricacid 3-MHA and 4-methyl hippuric acid 4-MHA; and (2) mixing a part of the first sample liquid with a third solvent so as to obtain a second sample liquid, detecting a second generation of metabolin through the liquid chromatogram tandem mass spectrum, wherein the second generation of metabolin comprises hippuric acid HA. By adopting the method disclosed by the invention, more than six metabolins can be detected in a combined manner.

Description

Technical field [0001] The invention relates to a method for detecting metabolites in urine, in particular to a method for detecting metabolites of benzene, toluene and xylene in urine. Background technique [0002] Biological monitoring refers to the monitoring of the content of chemical substances and their metabolites in human biological materials (blood, urine, etc.) or the level of biological effects produced by them, which can reflect the total exposure of chemical substances from different pathways and sources of the body. Therefore, biological monitoring can provide the actual exposure level of the body, which is more advantageous than environmental monitoring. [0003] Benzene compounds are chemicals that are widely used in industry and can be used as chemical raw materials, solvents, diluents, fuels, etc. Benzene compounds are also a common environmental pollutant. Among benzene compounds, benzene, toluene and xylene are the most common. Benzene has hematological toxic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/89
CPCG01N30/89
Inventor 乔荷刘晓东李珏孟祥娟牛东升丁晓文
Owner THE BEIJING PREVENTION & TREATMENT HOSPITAL OF OCCUPATIONAL DISEASE FOR CHEM IND
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