Method for detecting benzene, methylbenzene and xylene metabolin in urine

A detection method and technology for metabolites, applied in the detection of toluene and xylene metabolites, and the field of benzene in urine, can solve the problem of inability to comprehensively detect metabolites of benzene compounds, and achieve the goal of reducing matrix interference and improving measurement accuracy. Effect
CN109342633AActive Publication Date: 2019-02-15THE BEIJING PREVENTION & TREATMENT HOSPITAL OF OCCUPATIONAL DISEASE FOR CHEM IND
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
THE BEIJING PREVENTION & TREATMENT HOSPITAL OF OCCUPATIONAL DISEASE FOR CHEM IND
Publication Date
2019-02-15

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Abstract

The invention discloses a method for detecting benzene, methylbenzene and xylene metabolins in urine. The method comprises the following steps: (1) mixing urine with a first solvent, centrifuging so as to obtain supernate, mixing the supernate with a second solvent so as to obtain a first sample liquid, and detecting a first generation of metabolin in the first sample liquid through a liquid chromatogram tandem mass spectrum, wherein the first generation of metabolin comprises phenylmercapturic acid acid S-PMA, 8-hydroxy-2-deoxyguanosine 8-OHdG, 2-methyl hippuric acid 2-MHA, 3-methyl hippuricacid 3-MHA and 4-methyl hippuric acid 4-MHA; and (2) mixing a part of the first sample liquid with a third solvent so as to obtain a second sample liquid, detecting a second generation of metabolin through the liquid chromatogram tandem mass spectrum, wherein the second generation of metabolin comprises hippuric acid HA. By adopting the method disclosed by the invention, more than six metabolins can be detected in a combined manner.
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Description

technical field

[0001] The invention relates to a method for detecting metabolites in urine, in particular to a method for detecting metabolites of benzene, toluene and xylene in urine. Background technique

[0002] Biological monitoring refers to monitoring the content of chemical substances and their metabolites in human biological materials (blood, urine, etc.) or the level of biological effects produced by them, which can reflect the total exposure of the body to chemical substances from different routes and sources. Therefore, biological monitoring can provide the actual exposure level of the body and thus has advantages over environmental monitoring.

[0003] Benzene compounds are widely used chemicals in industry, and can be used as chemical raw materials, solvents, diluents, fuels, etc. Benzene compounds are also a common type of environmental pollutants. Among the benzene series compounds, benzene, toluene and xylene are the most common. Benzene has blood toxicit...

Claims

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