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Method for detecting benzene, methylbenzene and xylene metabolin in urine

A detection method and technology for metabolites, applied in the detection of toluene and xylene metabolites, and the field of benzene in urine, can solve the problem of inability to comprehensively detect metabolites of benzene compounds, and achieve the goal of reducing matrix interference and improving measurement accuracy. Effect

Active Publication Date: 2019-02-15
THE BEIJING PREVENTION & TREATMENT HOSPITAL OF OCCUPATIONAL DISEASE FOR CHEM IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above method uses solid phase extraction to separate metabolites. Due to the limitation of extraction conditions, only a limited number of four metabolites can be detected, so it is impossible to comprehensively detect metabolites of benzene compounds.

Method used

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  • Method for detecting benzene, methylbenzene and xylene metabolin in urine
  • Method for detecting benzene, methylbenzene and xylene metabolin in urine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Sample handling:

[0069] Put 300 μL of urine in a centrifuge tube, add 900 μL of methanol, vortex and shake evenly, and centrifuge at 15000 rpm for 15 min to obtain a supernatant. Put 500 μL of the supernatant into a new centrifuge tube, add 2 mL of ultrapure water, and vortex evenly to obtain the first sample solution. Put 1mL of the first sample solution into a 2mL sampling bottle and inject to analyze the contents of S-PMA, 8-OHdG, 2-MHA, 3-MHA and 4-MHA.

[0070] Both 20 μL of the first sample solution and 1980 μL of 15 vol% methanol aqueous solution were placed in a new centrifuge tube, and vortexed evenly to obtain the second sample solution. Put 1mL of the second sample solution into a 2mL sampling bottle, and inject to analyze the content of HA.

[0071] Standard solution preparation:

[0072] Using 15vol% methanol in water as a solvent, prepare mixed standard solutions of different concentrations. The concentration range of 8-OHdG: 0.002~2ng / mL; the concen...

Embodiment 2

[0087] Sample handling:

[0088] Put 300 μL of urine in a centrifuge tube, add 900 μL of methanol, vortex and shake evenly, and centrifuge at 15000 rpm for 15 min to obtain a supernatant. Put 500 μL of the supernatant into a new centrifuge tube, add 2 mL of ultrapure water, and vortex evenly to obtain the first sample solution. Put 1mL of the first sample solution into a 2mL sampling bottle and inject to analyze the content of S-PMA, t,t-MA, 8-OHdG, 2-MHA, 3-MHA and 4-MHA.

[0089] Both 20 μL of the first sample solution and 1980 μL of 15 vol% methanol aqueous solution were placed in a new centrifuge tube, and vortexed evenly to obtain the second sample solution. Put 1mL of the second sample solution into a 2mL sampling bottle, and inject to analyze the content of HA.

[0090] Standard solution preparation:

[0091] Using 15vol% methanol in water as a solvent, prepare mixed standard solutions of different concentrations. The concentration range of 8-OHdG: 0.002~2ng / mL; the...

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Abstract

The invention discloses a method for detecting benzene, methylbenzene and xylene metabolins in urine. The method comprises the following steps: (1) mixing urine with a first solvent, centrifuging so as to obtain supernate, mixing the supernate with a second solvent so as to obtain a first sample liquid, and detecting a first generation of metabolin in the first sample liquid through a liquid chromatogram tandem mass spectrum, wherein the first generation of metabolin comprises phenylmercapturic acid acid S-PMA, 8-hydroxy-2-deoxyguanosine 8-OHdG, 2-methyl hippuric acid 2-MHA, 3-methyl hippuricacid 3-MHA and 4-methyl hippuric acid 4-MHA; and (2) mixing a part of the first sample liquid with a third solvent so as to obtain a second sample liquid, detecting a second generation of metabolin through the liquid chromatogram tandem mass spectrum, wherein the second generation of metabolin comprises hippuric acid HA. By adopting the method disclosed by the invention, more than six metabolins can be detected in a combined manner.

Description

technical field [0001] The invention relates to a method for detecting metabolites in urine, in particular to a method for detecting metabolites of benzene, toluene and xylene in urine. Background technique [0002] Biological monitoring refers to monitoring the content of chemical substances and their metabolites in human biological materials (blood, urine, etc.) or the level of biological effects produced by them, which can reflect the total exposure of the body to chemical substances from different routes and sources. Therefore, biological monitoring can provide the actual exposure level of the body and thus has advantages over environmental monitoring. [0003] Benzene compounds are widely used chemicals in industry, and can be used as chemical raw materials, solvents, diluents, fuels, etc. Benzene compounds are also a common type of environmental pollutants. Among the benzene series compounds, benzene, toluene and xylene are the most common. Benzene has blood toxicit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/89
CPCG01N30/89
Inventor 乔荷刘晓东李珏孟祥娟牛东升丁晓文
Owner THE BEIJING PREVENTION & TREATMENT HOSPITAL OF OCCUPATIONAL DISEASE FOR CHEM IND
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