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Method for detecting methyl hippuric acid in urine

A methylhippuric acid and detection method technology, applied in the field of biological analysis, can solve problems such as unspecified biological limits, and achieve high recovery, high sensitivity, and good stability

Inactive Publication Date: 2015-10-21
DONGCHEN LEADER TESTING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on the metabolites of p-xylene urine in my country is still in its infancy, and the air exposure limit value of xylene has been established, but the corresponding biological limit value has not yet been established

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The detection method of methylhippuric acid in urine comprises the following steps:

[0021] Step 1, take a urine sample, add p-chlorobenzoic acid, hydroquinone, 3,4-di-p-hydroxyphenylalanine, shake and mix, and centrifuge;

[0022] Step 2, adding hydrochloric acid solution, sodium chloride and ethyl acetate to the supernatant obtained by centrifuging in step 1, shaking and mixing, and centrifuging;

[0023] Step 3, take the supernatant obtained by centrifuging in step 2, and rotate to evaporate;

[0024] Step 4, add the residue obtained in step 3 to distilled water, concentrate by nanofiltration, and the concentrated solution is analyzed by high performance liquid phase.

[0025] In step 1, the amount of urine is 50 mL, the amount of p-chlorobenzoic acid is 1 g, the amount of hydroquinone is 2 g, and the amount of 3,4-di-p-hydroxyphenylalanine is 1 g.

[0026] In step 1, the centrifugation condition is 5000rpm, and the centrifugation time is 5min.

[0027] In step 2...

Embodiment 2

[0034] The detection method of methylhippuric acid in urine comprises the following steps:

[0035] Step 1, take a urine sample, add p-chlorobenzoic acid, hydroquinone, 3,4-di-p-hydroxyphenylalanine, shake and mix, and centrifuge;

[0036] Step 2, adding hydrochloric acid solution, sodium chloride and ethyl acetate to the supernatant obtained by centrifuging in step 1, shaking and mixing, and centrifuging;

[0037] Step 3, take the supernatant obtained by centrifuging in step 2, and rotate to evaporate;

[0038] Step 4, add the residue obtained in step 3 to distilled water, concentrate by nanofiltration, and the concentrated solution is analyzed by high performance liquid phase.

[0039] In step 1, the amount of urine was 75 mL, the amount of p-chlorobenzoic acid was 3 g, the amount of hydroquinone was 5 g, and the amount of 3,4-di-p-hydroxyphenylalanine was 2 g.

[0040] In step 1, the centrifugation condition is 6000rpm, and the centrifugation time is 4min.

[0041] In st...

Embodiment 3

[0048] The detection method of methylhippuric acid in urine comprises the following steps:

[0049] Step 1, take a urine sample, add p-chlorobenzoic acid, hydroquinone, 3,4-di-p-hydroxyphenylalanine, shake and mix, and centrifuge;

[0050] Step 2, adding hydrochloric acid solution, sodium chloride and ethyl acetate to the supernatant obtained by centrifuging in step 1, shaking and mixing, and centrifuging;

[0051] Step 3, take the supernatant obtained by centrifuging in step 2, and rotate to evaporate;

[0052] Step 4, add the residue obtained in step 3 to distilled water, concentrate by nanofiltration, and the concentrated solution is analyzed by high performance liquid phase.

[0053]In step 1, the amount of urine was 100 mL, the amount of p-chlorobenzoic acid was 5 g, the amount of hydroquinone was 6 g, and the amount of 3,4-di-p-hydroxyphenylalanine was 4 g.

[0054] In step 1, the centrifugation condition is 7000rpm, and the centrifugation time is 3min.

[0055] In st...

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Abstract

The invention provides a method for detecting methyl hippuric acid in urine. The method comprises the following steps: taking a urine sample, adding parachlorobenzoic-acid, hydroquinone and 3,4-dihydroxyphenylanaline into the urine sample, and oscillating, mixing and centrifuging; then adding a hydrochlric solution, sodium chloride and ethyl acetate into a supernatant obtained through centrifugation, and oscillating, mixing and centrifuging; then conducting rotary evaporation on a supernatant obtained through centrifugation; finally, adding residues obtained in the step 3 into distilled water, concentrating by nanofiltration, and conducting efficient liquid phase analysis on the concentrated solution. The method provided by the invention is high in sensitivity, precision and recovery rate, and excellent in stability; all methodological indexes conform to related requirements of Guide for Establishing Occupational Health Standards Part 5-Method for Detecting Chemical Substances in Biomaterials; the method provided by the invention can be used for detecting 3 isomers of methyl hippuric acid in urine of both occupational workers exposed to dimethylbenzene and non-occupational people exposed to dimethylbenzene.

Description

technical field [0001] The invention belongs to biological analysis methods, in particular to a method for detecting methylhippuric acid in urine. Background technique [0002] Xylene is a colorless and transparent liquid with a special smell of aromatic hydrocarbons. It is a product in which two hydrogens on the benzene ring are replaced by methyl groups. There are three isomers: ortho, meta and para. Xylene is volatile, particularly smelly, flammable, can be mixed with ethanol, chloroform or ether, and is insoluble in water. As a very important chemical raw material, industrial xylene refers to 3 kinds of meta-xylene of 45% to 70%, 15% to 25% of p-xylene and 10% to 15% of ortho-xylene. A mixture of conformers. [0003] In industry, xylene is mainly used in paints and their thinners, adhesives and pharmaceutical industries, as well as additives for various coatings, high-efficiency antiknock agents for aviation fuels, synthesis of fibers, plastics, rubber, dyes, and p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/89G01N30/12
Inventor 熊开胜张海防谢建庭
Owner DONGCHEN LEADER TESTING
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