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A kind of mof-based fluorescent probe and its preparation method and application

A fluorescent probe and MOF technology, applied in the field of fluorescent sensors, can solve the problems of low sensitivity, cumbersome operation, and long time consumption

Active Publication Date: 2019-05-24
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a MOF-based fluorescent probe and its preparation method and application in order to solve the problems of cumbersome operation, long time-consuming and low sensitivity in the detection of the existing concentration of hippuric acid

Method used

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  • A kind of mof-based fluorescent probe and its preparation method and application
  • A kind of mof-based fluorescent probe and its preparation method and application
  • A kind of mof-based fluorescent probe and its preparation method and application

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preparation example Construction

[0027] The present invention firstly provides a kind of preparation method of the fluorescent probe based on MOF, this method comprises:

[0028] Step 1: will contain Al 3+ Add the metal ions and 2-aminoterephthalic acid into deionized water, mix and stir evenly, and then react at 120-160°C. After the reaction is completed, centrifuge, wash, and dry to obtain MOF solid powder; the Al 3+ The metal ion is preferably aluminum nitrate or aluminum chloride; 3+ The molar ratio of metal ions, 2-aminoterephthalic acid and deionized water is preferably (0.9~1.1):(0.9~1.1):(1.5~2). The reaction temperature is preferably 130-140°C, and the reaction time is preferably 4-6h;

[0029] Step 2: The MOF solid powder obtained in Step 1, carboxylated calix[4]arene, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinyl Add the imine to deionized water and stir, filter, wash, and dry to obtain a fluorescent probe based on MOF; the stirring temperature is preferably r...

Embodiment 1

[0035] 1) Synthesis of fluorescent probe: AlCl 3 ·6H 2 O (1mmol) and 2-aminoterephthalic acid (1mmol) were dissolved in 30mL deionized water, mixed and stirred evenly, and reacted at a constant temperature of 150°C for 5h; after the reaction was completed, centrifuged, washed, and dried to obtain a solid powder; deionized water was used as the reaction Medium, 20 mg of solid powder prepared in the above steps, 50 mg of carboxylated calix[4]arene, 50 mg of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, and 50 mg of N-hydroxysuccinate 20 mg of imide was stirred at room temperature for 30 min, filtered, washed, and dried to obtain a fluorescent probe. Characterization of fluorescent probes by powder X-ray diffraction, as figure 1 a.

[0036] 2) Sample preparation:

[0037] Take the urine sample in a centrifuge tube, the centrifugation condition is 4500rpm, and the centrifugation time is 5 minutes. Take the supernatant, filter it through a 0.45μm water filter mem...

Embodiment 2

[0045] 1) Synthesis of fluorescent probe: AlCl 3 ·6H 2 O (1mmol), 2-aminoterephthalic acid (1.1mmol) were dissolved in 29mL deionized water and mixed and stirred evenly; 5g was reacted at a constant temperature of 130°C; after the reaction was completed, centrifuged, washed, and dried to obtain a solid powder; deionized water was used as Reaction medium, take 22 mg of solid powder prepared in the above steps, 55 mg of carboxylated calix[4]arene, 52 mg of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, N-hydroxy 24 mg of succinimide was stirred at room temperature for 20 min, filtered, washed, and dried to obtain a fluorescent probe. Characterization of fluorescent probes by powder X-ray diffraction, as figure 1 b.

[0046] 2) Sample preparation:

[0047] Take the urine sample in a centrifuge tube, the centrifugation condition is 5000rpm, and the centrifugation time is 4 minutes. Take the supernatant, filter it through a 0.45μm water filter membrane, and put th...

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Abstract

The invention provides a fluorescent probe based on MOF, a preparation method and an application of same, belongs to the technical field of fluorescence sensors, and is used for solving problems detection of concentration of hippuric acid in the prior art has complex operations, long time consumption and low sensitivity. The preparation method includes the steps of: A) adding metal ions, containing Al<3+>, and 2-aminoterephthalic acid to deionized water, uniformly mixing and stirring the solution, performing a reaction at 120-160 DEG C to prepare MOF solid powder; B) adding the MOF solid powder, carboxylated calix[4]arene, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide to deionized water, and stirring the liquid to produce the fluorescent probe based on MOF. The invention also provides an application of the fluorescent probe based on MOF as a fluorescence sensor in detection of hippuric acid. The fluorescent probe has high fluorescent emitting efficiency and good fluorescent stability, is convenient and quick to use in detection and has high sensitivity and good stability.

Description

technical field [0001] The invention belongs to the technical field of fluorescence sensors, and in particular relates to a metal-organic framework (MOF)-based fluorescent probe and a preparation method and application thereof. Background technique [0002] Toluene is a widely used solvent, but its toxicity is relatively high. Studies have shown that people exposed to toluene for a long time are prone to neurobehavioral disorders. Therefore, the monitoring of toluene is very important in occupational health care. However, due to differences in individual physique, monitoring the level of toluene in the environment cannot accurately estimate the actual poisoning level of personnel. Hippuric acid is a metabolite of the human body after exposure to toluene, and it is a biological detection indicator of exposure to toluene. Approximately 80% of inhaled toluene is excreted in the urine as hippuric acid, and the concentration of hippuric acid in the urine is directly proportion...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/02C09K11/06G01N21/64
CPCC09K11/025C09K11/06C09K2211/186G01N21/6486
Inventor 贾琼杜亚冉郑海娇王荟琪郭会华江丹丹张文娟杨巾栏屈淑梅
Owner JILIN UNIV
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