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Microbial compound fungicide for treating oil base drilling cuttings as well as preparation method and application of microbial compound fungicide

A technology of composite bacterial agent and oil-based drilling cuttings, applied in the field of microorganisms, can solve the problems of lack of application of microbial treatment technology, high treatment costs, serious secondary pollution, etc., and achieve the expansion of application range, harmless treatment, and prolong service life Effect

Active Publication Date: 2014-09-24
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, physical and chemical treatment technology is a relatively mature method commonly used at home and abroad, but most of these technologies have problems such as high treatment costs, serious secondary pollution, and poor universality; biological treatment technology is considered to be the most economical and sustainable. Environmentally Friendly Technology
This technology started earlier in foreign countries and has been widely used. However, the application of microbial treatment technology for oil-based drilling cuttings in China is still relatively scarce. Therefore, the development of new and efficient microbial treatment technology and application research for oil-based drilling cuttings is crucial It is of great significance to carry out the normal exploration and development of oil and gas fields, and to protect the safe, green, harmonious and sustainable production of oil and gas fields

Method used

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  • Microbial compound fungicide for treating oil base drilling cuttings as well as preparation method and application of microbial compound fungicide
  • Microbial compound fungicide for treating oil base drilling cuttings as well as preparation method and application of microbial compound fungicide
  • Microbial compound fungicide for treating oil base drilling cuttings as well as preparation method and application of microbial compound fungicide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The bacterial strain involved in the composite bacterial agent is obtained by screening with the selective medium method, and the specific steps are as follows:

[0042] (1) Take 10g soil sample in the habitat polluted by oil-based drilling cuttings.

[0043] (2) Add 10 g of soil sample to 200 mL of selective liquid medium for shaking (35°C, 120 rpm) and cultivate for 30 days. The formula of selective liquid medium is: 5 g of glucose, 0.5 g of beef extract, 2 g of peptone, chloride Sodium 2g, diesel oil 20g, water 1000mL, medium pH 6.5.

[0044](3) Take the 30-day culture medium, and select highly active strains—Pseudomonas aeruginosa and Acinetobacter baumannii—by plate streak separation on selective solid medium. The formula of selective solid medium is: glucose 5g, beef extract 0.5g, peptone 2g, sodium chloride 2g, diesel oil 20g, water 1000mL, agar 20g, medium pH6.5.

Embodiment 2

[0046] (1) Quantify the beef extract peptone solid medium that has been subjected to high-temperature sterilization (121°C, 30 minutes) for standby use. The formula of the beef extract peptone solid medium is 20g of peptone, 10g of beef extract, 20g of glucose, 1000mL of water, and 15- 20g, pH6.5-7.0.

[0047] (2) The strains X7 and X11 were respectively inserted into sterilized (121° C., 30 minutes) beef extract-peptone solid slant medium for activation.

[0048] (3) Quantitatively prepare the liquid medium that has been sterilized at high temperature (121°C, 30 minutes). The components of the liquid medium include: glucose 10g, beef extract 1g, peptone 5g, sodium chloride 5g, diesel oil 20g, water 1000mL , pH6.5-7.0.

[0049] (4) The activated strains were respectively inserted into the liquid medium for shaking (35° C., 140 rpm) and cultured for 24 hours.

[0050] (5) Counting the cultured bacterium liquid, adjusting the bacterial content of the second bacterial strain to...

Embodiment 3

[0052] The method for preparing a liquid microbial composite bacterial agent is the same as that of Example 1, except that the composition of the liquid medium includes: glucose 5g, beef extract 0.5g, peptone 2g, sodium chloride 2g, diesel oil 30g, water 1000mL, pH6.5 -7.0; the volume ratio of X7:X11 is 6:1.

[0053] After preparing the liquid microbial composite bacterial agent, add dry bran therein as the adsorbent, and the addition is: every 1L liquid microbial composite bacterial agent adds 1Kg of adsorbent, and then dries in a constant temperature incubator at 35°C (moisture content 15%-20%), and then add 5g of yeast extract powder per kilogram of dry bacterial agent as an accelerator, stir evenly, and finally pack and store at low temperature (4°C-10°C).

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Abstract

The invention relates to a microbial compound fungicide for treating oil base drilling cuttings as well as a preparation method and application of the microbial compound fungicide. The compound fungicide comprises pseudomonas aeruginosa with a collection number of CGMCC NO:8983 and acinetobacter baumannii with the collection number of CGMCCNO:8984. The invention further discloses the preparation method of the microbial compound fungicide and application of the compound fungicide in treatment of oil base drilling cuttings. The preparation method includes preparation methods of liquid and solid fungicides.

Description

technical field [0001] The invention relates to a microbial composite bacterial agent for treating oil-based drilling cuttings, its preparation method and its application, and specifically relates to microorganisms constructed by using the interaction relations such as symbiosis, intergrowth and synergy among strains according to the principles of microbial ecology The compound microbial agent, its preparation method and its application belong to the field of microbiology. Background technique [0002] With the development of oil and gas exploration and development in China, more and more multilateral wells, horizontal wells and complex geological wells are drilled, and the problem of wellbore stability is becoming more and more prominent. As a result, the amount of waste oil-based pollutants produced has been increasing. Among them, oil-based drilling cuttings contain a large amount of pollutants, such as heavy metals and organic matter, and have been listed as national ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02C12R1/385C12R1/01
Inventor 闫志英袁月祥操卫平张博廉许力山李东刘晓风
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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