Kit and method for detecting Acinetobacter baumannii DNA

Abstract

The invention discloses a kit and method for detecting Acinetobacter baumannii DNA; the kit comprises: a nucleic acid release agent, a PCR (polymerase chain reaction) liquid, an enzyme mixed liquid, an Acinetobacter baumannii positive control, and an Acinetobacter baumannii negative control, wherein the PCR liquid is made from PCR buffer solution, deoxyribonucleoside, primers for amplifying targeted polynucleotide, a probe for amplifying targeted polynucleotide, primers for amplifying internally labeled fragments and a probe for detecting an internal label. By optimizing conditions in the embodiment of the invention, primers for amplifying targeted polynucleotide and probes for amplifying the targeted polynucleotide, both having optimal amplification, are screened out, the kit is capable of detecting not pathogens of Acinetobacter baumannii but various infections of Acinetobacter baumannii but, is highly specific, and accordingly has greatly improved detection sensitivity, accuracy and stability.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a kit and a detection method for detecting Acinetobacter baumannii DNA. Background technique [0002] At present, the laboratory diagnosis methods of Acinetobacter baumannii mainly use commercial bacterial identification systems, such as API20NE, VITED 2, Phoenix and MicroScan WalkAway, but the error rate is as high as 25%. Based on identification methods of biochemical and bacterial culture, the identification method of bacterial culture not only has low sensitivity, but its culture period is as long as 24-72 hours, which often causes patients to miss the best treatment period. [0003] The clinical detection of Acinetobacter baumannii-DNA mainly uses real-time fluorescent PCR technology. The improvement and application of this technology has developed rapidly in recent years. This technology integrates PCR, molecular hybridization and photochemical organically, s...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the invention of the present invention is to provide a test kit and detection method for detecting Acinetobacter baumannii DNA, to solve the problem of existing test kits for detecting Acinetobacter baumannii DNA based on real-time fluorescent PCR technology, detection process, and detection accuracy low problem

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