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Strain capable of degrading pyrethroid insecticide residues and application of strain

A technology of pyrethroids and insecticides, applied in the fields of environmental microorganisms and agriculture, can solve the problems of excessive pesticide residues and environmental pollution, protect the ecological environment and human health, and solve the problems of excessive residues and environmental pollution problem effect

Active Publication Date: 2017-08-18
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Direct application of this bacterial agent can reduce the residual amount of permethrin in water or soil by more than 85% in a short period of time, which can solve the problem of excessive residues of pyrethroid insecticides and environmental pollution in agricultural production, and produce non-toxic and pollution-free of green agricultural products

Method used

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  • Strain capable of degrading pyrethroid insecticide residues and application of strain
  • Strain capable of degrading pyrethroid insecticide residues and application of strain
  • Strain capable of degrading pyrethroid insecticide residues and application of strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1 Isolation and Identification of Bacterial Strain ZH-14

[0055] 1. Isolation and screening of strains

[0056] (1) Sample: collected from the activated sludge in the sewage treatment tank in Singapore.

[0057] (2) The separation and screening method adopts the enrichment culture method, as follows:

[0058] Take 5g of activated sludge sample and add it to 50mL basal medium. At the same time, add the mother liquor of chlorstrobin (acetone or methanol as solvent) to make the final mass concentration of permethrin 50mg / L. Carry out enrichment culture of degrading bacteria. After culturing for 7 days, transfer to the second batch of basal medium containing 100 mg / L permethrin according to 10% inoculation amount. After 7 days of culture under the same conditions, the 10% inoculum was transferred to the basal medium containing 200 mg / L permethrin, and the culture was continued for 7 days. By analogy, the mass concentration of permethrin was continuously doubled...

Embodiment 2

[0072] Example 2 Preparation of bacterial strain ZH-14 degrading bacterial agent

[0073] 1. The production process of using the above-mentioned strain ZH-14 to prepare the degrading bacterial agent is as follows: slant seed—shake flask seed solution—seed tank cultivation—production tank fermentation—product (the packaging dosage form is a liquid dosage form or a solid bacterial dosage form ).

[0074] 2. The specific method is as follows:

[0075] (1) Activate the strain ZH-14 on the LB solid plate and inoculate it on the inclined surface of the LB test tube for later use.

[0076] (2) inoculate the test tube seed of bacterial strain ZH-14 in the 1000mL shake flask that contains 250mL LB medium (medium formula is: yeast extract 0.5%, peptone 1%, NaCl 1%, pH value is 7.0~7.2), Shake at a constant temperature of 30°C to the logarithmic phase, and inoculate the obtained strains in a seed tank, which is filled with a seed medium with a liquid volume of 70%. 1.1Kg / cm after feed...

Embodiment 3

[0079] Example 3 ZH-14 bacterial agent optimal degradation process conditions

[0080] 1. Conduct a single-factor degradation test on the ZH-14 bacterial agent, and measure the degradation rate of the ZH-14 bacterial agent by sequentially changing the factors that affect growth and degradation, such as temperature, pH value, inoculum volume, liquid filling volume, and oscillation rate. , to determine the key factors affecting the degradation rate of ZH-14 inoculum.

[0081] Utilize SAS 9.0 statistical software to carry out experimental design (Table 2) according to the Response Surface Method Box-Behnken design principle, to influence the key factor pH value of bacterial strain ZH-14 growth and degradation (X 1 ), temperature (X 2 ) and inoculation volume (X 3 ) as the independent variable, the response value is the degradation rate of permethrin (Y 1 ), to establish a multiple quadratic regression equation.

[0082] According to the multiple quadratic regression equation fo...

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Abstract

The invention discloses a strain capable of degrading pyrethroid insecticide residues and an application of the strain. The strain is an Acinetobacter baumannii strain ZH-14 and is preserved in CCTCC (China Center for Type Culture Collection) on November 28, 2016, and the preservation number is CCTCC NO: M 2016689. The strain has remarkable capacity of degrading the pyrethroid insecticide residues, and a liquid preparation prepared from the strain is low in production cost, convenient to use and suitable for treating residual pollution caused by a pyrethroid insecticide in natural environment such as water or soil and the like; the quantity of pyrethroid insecticide residues in water or soil can be reduced by 85% or more in a short time after direct application of the preparation, the problem of standard exceeding of the pyrethroid insecticide residues in agricultural production and the problem of environmental pollution can be solved, non-toxic and non-pollution green agricultural products are produced, and the strain has important theoretical guidance and practical application value.

Description

technical field [0001] The invention belongs to the field of applied environmental microbes and agricultural technology. More specifically, it relates to a pyrethroid insecticide residual degradation strain and its production bacterial agent and optimal degradation process conditions. Background technique [0002] Pyrethroid pesticides are a class of highly hydrophobic biomimetic insecticides with broad-spectrum and high-efficiency insecticidal activity. They have been used in agricultural and residential pest control as early as the 1970s. At present, pyrethroid pesticides are the most As an important crop insecticide, the use of pyrethroid insecticides accounted for 38% of the pesticide market share in 2015. In the future, the market share and application area of ​​pyrethroids will continue to grow. [0003] In recent years, pyrethroid pesticide residues have been frequently detected in both agricultural and non-agricultural areas, which has attracted widespread attention...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02B09C1/10C02F3/34C12R1/01A62D101/04A62D101/28C02F101/34C02F101/36C02F101/38
CPCA62D3/02A62D2101/04A62D2101/28B09C1/10C02F3/34C02F2101/34C02F2101/36C02F2101/38C12N1/20C12N1/205C12R2001/01
Inventor 陈少华占卉冯彦媚范兴辉叶田滕诗雨何杰华
Owner SOUTH CHINA AGRI UNIV
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