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Marine Bacillus amyloliquefaciens and uses thereof

Amylolytic spore and marine technology, applied in the direction of bacteria, antibacterial drugs, medical preparations containing active ingredients, etc., can solve problems such as unsatisfactory effects, environmental pollution, residual aquatic species, etc., achieving good development prospects and remarkable effects Effect

Active Publication Date: 2015-10-14
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, chemical agents are often used in the prevention and control of aquatic products, but the effect is not ideal, and it will pollute the environment and remain in aquatic species.

Method used

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  • Marine Bacillus amyloliquefaciens and uses thereof
  • Marine Bacillus amyloliquefaciens and uses thereof
  • Marine Bacillus amyloliquefaciens and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example One. Obtaining Marine Bacillus amyloliquefaciens B01

[0040] 1. Sample collection: Collect the sludge and seawater of the Weihai sea cucumber breeding pond, enrich it and cultivate it for 20 days, then take it out and dilute it by 1000 times and incubate it in an incubator at 28 °C for 24 h.

[0041] 2. Isolate the bacteria: carefully pick out the bacteria with an inoculation needle, streak them for purification, and culture them in an incubator at 28 °C. If there are bacterial colonies of different shapes, sizes and colors, they need to be picked separately for further purification until each plate Present bacterial colonies with the same shape, size and color;

[0042] 3. Screening antagonistic bacteria: streak the above two aquatic pathogens to the center of the 2216E medium plate, connect the purified bacteria at four points at equal distances around the pathogens, culture in a 28 °C incubator, and observe the bacteriostasis for 1-2 days , It is found that ther...

Embodiment 2

[0060] Example 2: Preparation of marine Bacillus amyloliquefaciens B01 fermentation metabolic liquid, the specific process is as follows:

[0061] 1. Preparation of marine Bacillus amyloliquefaciens B01 seed solution: After activation, the marine Bacillus amyloliquefaciens B01 strain stored in a refrigerator at 4°C is inoculated into LANDY liquid medium (beef extract 3g, peptone 10g, sodium chloride 5g, Adjust pH 7.0-7.2, 1000ml of water, sterilize at 121°C for 20min), culture at 28°C, shake at 150rpm / min for 12h, as seed solution.

[0062] 2. Obtainment of marine Bacillus amyloliquefaciens B01 fermentation metabolite: the above seed solution was inoculated into LANDY liquid medium at an inoculum of 2%, and cultured at 28°C and 150rpm / min shaking for 48h to obtain fermentation broth. Centrifuge at 10000rpm / min for 10min, take the fermentation supernatant, filter it with a 0.2μm microporous membrane, and store at -20°C for later use.

Embodiment 3

[0063] Example 3 The inhibitory effect of fermentation broth (Example 2) on aquatic pathogens:

[0064] The agar diffusion method was used to detect the antagonistic activity of the fermentation supernatant. The above two aquatic pathogens were inoculated into the center of the 2216E medium plate by drawing lines, and incubated for a period of time at 28 °C. Use a punch with a hole diameter of 8 mm to punch holes in the pathogens and number them. Add 100 μl B01 to each hole. Filter the sterilized fermentation supernatant and incubate at 28°C for a period of time to observe its antibacterial effect. The result is image 3 Shown.

[0065] It can be seen from the figure that the fermentation supernatant of marine Bacillus amyloliquefaciens B01 can effectively inhibit a variety of aquatic disease bacteria such as Acinetobacter baumannii and Vibrio parahaemolyticus.

[0066] In summary, the present invention obtained a strain of marine Bacillus amyloliquefaciens ( Bacillus amyloliquefa...

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Abstract

The present invention discloses a strain of marine Bacillus amyloliquefaciens B01, wherein the strain is preserved in the China general microbiological culture collection center on December 11, 2014, and has the preservation number of CGMCC No.10154. According to the present invention, the fermentation broth supernatant of the Bacillus amyloliquefaciens B01 can inhibit aquatic diseases caused by a variety of pathogenic bacteria such as Acinetobacter baumannii, Vibrio parahaemolyticus and the like, and the marine Bacillus amyloliquefaciens B01 has characteristics of no toxicity on human and animals, green environmental protection, aquatic pollution disease prevention and control effect, and good biological pesticide development prospects.

Description

technical field [0001] The invention relates to the field of aquaculture, in particular to a marine bacillus amyloliquefaciens and its application. Background technique [0002] In recent decades, driven by the limitation of wild resources and market demand, my country's aquaculture industry has developed continuously and rapidly. It is currently the only country in the world where the production of aquaculture exceeds that of fishing. Aquaculture has become a major part of my country's national economy. The pillar industry continues to provide my country with a large amount of high-quality protein, and may become a key factor in solving my country's food security problems. [0003] With the rapid development of my country's aquaculture industry, large-scale and intensive farming models have gradually replaced extensive, semi-extensive, and semi-intensive farming models. The transformation of the breeding mode has brought disasters to the breeding industry while bringing hig...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/742A61P31/04C12R1/07
CPCA61K35/742C12N1/205C12R2001/07
Inventor 杜宗军穆大帅陈冠军
Owner SHANDONG UNIV
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