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Bacillus subtilis 9A and application thereof

A technology of Bacillus subtilis and inoculum, applied in the field of agricultural microorganisms, can solve problems such as safety problems, environmental pollution pesticide residues, etc., and achieve the effect of good development prospects of biological pesticides

Inactive Publication Date: 2014-04-30
INST OF PLANT PROTECTION GUANGXI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the disease is mainly controlled by chemical agents, but long-term use of chemical pesticides will cause pathogenic bacteria to develop drug resistance, and bring about environmental pollution and safety problems of pesticide residues. Therefore, the development and utilization of biocontrol bacteria that exist in nature has important practical significance

Method used

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  • Bacillus subtilis 9A and application thereof
  • Bacillus subtilis 9A and application thereof
  • Bacillus subtilis 9A and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Isolation, screening and identification of Bacillus subtilis 9A

[0030] 1.1 Isolation of bacteria

[0031] Use a sterilized writing brush to swipe on the powdery mildew lesions of melon leaves, and the falling objects fall on the PDA medium plate, and place them in an incubator at 26°C for natural light cultivation. Observe regularly during the cultivation period, purify bacterial colonies with obvious differences in appearance, shape and color, and save them for future use.

[0032] PDA medium formula: potato 200g, glucose 10g, agar 16g, distilled water 1000ml.

[0033] 1.2 Screening and obtaining of antagonistic strain 9A

[0034] Preliminary screening: Antagonistic screening of the bacterial strains isolated and purified in 1.1 was carried out on a PDA plate by using the plate confrontation growth method with a strain of Anthracnose mangoicum as an indicator bacterium, and the strains with antagonistic effects were preserved for future use.

[0035] Re-...

Embodiment 2

[0041] The preparation of embodiment 2 bacillus subtilis 9A fermented liquid

[0042] (1) Strain activation: Inoculate Bacillus subtilis 9A on solid medium and culture at 25°C-28°C for 1 day;

[0043] (2) Fermented seed culture: Inoculate one loop of the above-mentioned activated bacterial lawn into 100ml of seed culture liquid medium, and vibrate at 180r / m for 12 hours at 25°C-28°C to obtain seed liquid;

[0044] (3) Bacterial agent fermentation: Inoculate the above seed liquid into the bacterial agent fermentation culture medium at an inoculum amount of 0.05% (V / V), and culture at 180r / m at 25°C-28°C for 48 hours to obtain subtilis spores Bacillus 9A bacterial agent (the number of viable bacteria is about 3.0×10 9 cfu / ml).

[0045] The formula of solid medium for strain activation culture is: beef extract 5.0g, peptone 10.0g, NaCl 5.0g, distilled water 1000mL, agar powder 15g, pH7.0-7.2.

[0046] The formula of the liquid culture medium for strain fermented seed culture i...

Embodiment 3

[0048]The preparation of embodiment 3 Bacillus subtilis 9A fermented liquid

[0049] (1) Strain activation: Inoculate Bacillus subtilis 9A on solid medium and culture at 25°C-28°C for 2 days;

[0050] (2) Fermented seed culture: Inoculate a loop of the lawn of the above-mentioned activated strain into 100ml of seed culture liquid medium, and vibrate at 25°C-28°C at 180r / m for 24 hours to obtain a seed liquid;

[0051] (3) Bacterial agent fermentation: Inoculate the above seed liquid into the bacterial agent fermentation culture medium at an inoculum amount of 0.1% (V / V), and vibrate at 180r / m at 25°C-28°C for 72 hours to obtain Bacillus subtilis Bacillus 9A bacterial agent (the number of viable bacteria is about 8.0×10 9 cfu / ml).

[0052] The formula of solid medium for strain activation culture is: beef extract 5.0g, peptone 10.0g, NaCl 5.0g, distilled water 1000mL, agar powder 15g, pH7.0-7.2.

[0053] The formula of the liquid culture medium for strain fermented seed cult...

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Abstract

The invention relates to bacillus subtilis 9A and application thereof. The bacillus subtilis 9A has the collection number of CCTCC No. M2012493, and a method for preparing a bacillus subtilis 9A agent is provided. The bacillus subtilis 9A and the agent thereof have the effects of inhibiting pathogenic bacteria growth of mango anthracnose, has the powerful inhibition effect even killing effect on the conidiospore, a protective film layer can be formed on the surface of an application object to prevent the pathogenic bacteria from intruding, the occurrence rate of mango anthracnose is effectively reduced, and the bacillus subtilis 9A does not have harm to mango, is environment-friendly and has good biopesticide development prospect.

Description

technical field [0001] The invention relates to the technical field of agricultural microorganisms, in particular to a bacillus subtilis 9A and an application thereof. Background technique [0002] Mango anthracnose caused by Colletotrichum gloeosporioides is one of the main diseases in mango production, and it occurs in every mango producing area in the world. [0003] The fungus can infect the leaf tips, flower spikes and fruits of mangoes, causing dead shoots, leaf spots, fallen leaves, flower and fruit drop, etc., and the fungus has latent infection characteristics, and can cause black spots on the peel during postharvest storage and transportation. Plaque and fruit rot greatly shorten the shelf life of commercial mangoes. [0004] At present, the disease is mainly controlled by chemical agents, but long-term use of chemical pesticides will cause pathogenic bacteria to develop drug resistance, and bring about environmental pollution and safety problems of pesticide resi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/125
Inventor 莫贱友潘朝勃李其利郭堂勋黄穗萍
Owner INST OF PLANT PROTECTION GUANGXI ACADEMY OF AGRI SCI
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