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This utility model relates to the field of testing equipment technology, and in particular to a glass substrate testing device, including a frame, a support platform mounted on the frame and provided with a positioning surface for fixing the surface of the material to be tested, a testing camera assembly mounted above the support platform, the testing camera assembly being movable relative to the support platform, and the lens of the testing camera assembly reciprocating on the positioning surface, a positioning and locking mechanism being mounted at the edge of the positioning surface and assembled to position at least one side of the surface of the material to be tested, and an elastic pressing positioning unit connected to the testing camera assembly, capable of pressing the upper surface of the material to be tested so that the upper surface of the material to be tested is perpendicular to the lens direction of the testing camera assembly. This device can improve testing accuracy and efficiency, and can accurately acquire internal morphological information of micropores, as well as measure key parameters of micropores.