Method for analyzing acridine ester labeled antigen or acridine ester labeled antibody

A technology of labeling antigen and labeling antibody, which is applied in the direction of analyzing materials, analyzing materials through chemical reactions, and material inspection products, etc. It can solve the problems of poor repeatability of test results, long operation time, high false detection rate, etc. Sensitivity and reaction rate, good reproducibility, and accurate detection results

Inactive Publication Date: 2009-12-23
BEIJING ELCOTEQ BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] At present, the enzymatic chemiluminescence diagnostic products are still at the semi-automatic level, the operation time is long, the test results are poor in repeatability, and the false detection rate is high.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] In the labeling technology of acridinium ester, the present invention adopts a special process to avoid the variable region of the antibody that reacts with the antigen, and label the acridine ester on the fixed region of the antibody, so that it maintains its due characteristics, Not easy to lose. In further development, horseradish peroxidase, alkaline phosphatase and acridine ester should be labeled on the antigen (antibody) at the same time, so as not to affect the activity of the antigen (antibody), the activity of the enzyme, and the mutual Co-labeling in the presence of interference effects.

[0052] The present invention adopts the coating technology based on the biotin-avidin reaction, which can greatly increase the concentration of the antibody involved in the immune reaction, thereby improving the detection sensitivity and the reaction rate. The application of biotin-avidin system technology to the coating of magnetic particles is the company's technical opt...

Embodiment 2

[0059] Thyroxine (T4) Immunodiagnostic Kit

[0060] Using streptavidin-biotin reaction amplification system, combined with polystyrene coating technology, horseradish peroxidase-labeled antigen (antibody) uses streptavidin-biotin reaction amplification system to make the immune response The antibody concentration is greatly increased, which in turn improves detection sensitivity and reaction rate.

[0061] In the technical composition of using polystyrene coating, acridinium ester labeling antigen (antibody), the acridinium ester is labeled on the fixed area of ​​the antibody, so that in the coating technology of polystyrene solid phase, it labels the antibody It not only maintains its proper characteristics, but also is not easy to be deactivated

[0062] T4 antigen was labeled and purified by acridinium ester labeling process. In the coating technology of magnetic particles, it is labeled with horseradish peroxidase, and the corresponding antibody or antigen can be detecte...

Embodiment 3

[0068] 1. Preparation of acridinium ester label:

[0069] The acridinium ester label has a special group that produces luminescence in its chemical structure, and directly participates in the luminescence reaction in the process of luminescence immunoassay. Usually, these substances have no background luminescence, and can be used to detect low or trace concentrations of samples in the reaction, and are a class of luminescent agents with high luminescence efficiency. Acridinium ester I, II molecules and acridine amide III can be combined with antibodies (or antigens) to generate labeled antibodies with strong chemiluminescence activity and high specificity of immune response. The acridine ester is labeled on the amino group of the antigen (antibody). There are several amino groups in the variable region of the antibody that react with the antigen. These amino groups determine the specificity and affinity of the immune response. Therefore, in order to efficiently label the acr...

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Abstract

The invention relates to a chemiluminescent immunoassay method, in particular to a method for analyzing an acridine ester labeled antigen or an acridine ester labeled antibody and an immunoassay kit prepared by same. An acridine ester label is provided with a special luminescent group in a chemical structure, and the special group directly participates in luminescent reaction in the analyzing process of luminescent immunization; the substance does not have background luminescence usually, can be used for detecting a sample with low concentration or trace concentration in the reaction and is a luminescent agent with high luminescent efficiency; and molecules of acridine ester I and acridine ester II and acridine amide III can be combined with an antibody (antigen) to generate the labeled antibody with high chemiluminescent activity and immunological reaction specificity.

Description

Technical field: [0001] The present invention relates to a chemiluminescence immunoassay method, in particular to a method for analyzing an antigen or antibody labeled with acridinium ester, and an immunoassay kit prepared by the method. Background technique: [0002] Chemiluminesent Immunoassay (CLIA) is an emerging assay developed by immunoassay technology following enzyme immunoassay (EIA), radioimmunoassay (RIA), fluorescence immunoassay (FIA) and time-resolved fluorescence immunoassay (TRFIA). technology. [0003] Chemiluminescence technology is a measurement method developed in the past two or three decades. It uses the free energy released by chemical reactions to excite intermediates (commonly used alkaline phosphatase-amantadine), making it return from the excited state to the ground state, When the intermediate returns from the excited state to the ground state, photons of the same energy level are released, and the photons are measured for quantitative analysis. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N33/532G01N21/76
Inventor 林斯
Owner BEIJING ELCOTEQ BIO TECH
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