Method for analyzing acridine ester labeled antigen or acridine ester labeled antibody

A technology of labeling antigen and labeling antibody, which is applied in the direction of analyzing materials, analyzing materials through chemical reactions, and material inspection products, etc. It can solve the problems of poor repeatability of test results, long operation time, high false detection rate, etc. Sensitivity and reaction rate, good reproducibility, and accurate detection results
CN101609090AInactive Publication Date: 2009-12-23BEIJING ELCOTEQ BIO TECH
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
BEIJING ELCOTEQ BIO TECH
Publication Date
2009-12-23
Estimated Expiration
Not applicable Β· inactive patent
Patent Text Reader

Abstract

The invention relates to a chemiluminescent immunoassay method, in particular to a method for analyzing an acridine ester labeled antigen or an acridine ester labeled antibody and an immunoassay kit prepared by same. An acridine ester label is provided with a special luminescent group in a chemical structure, and the special group directly participates in luminescent reaction in the analyzing process of luminescent immunization; the substance does not have background luminescence usually, can be used for detecting a sample with low concentration or trace concentration in the reaction and is a luminescent agent with high luminescent efficiency; and molecules of acridine ester I and acridine ester II and acridine amide III can be combined with an antibody (antigen) to generate the labeled antibody with high chemiluminescent activity and immunological reaction specificity.
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Description

Technical field:

[0001] The present invention relates to a chemiluminescence immunoassay method, in particular to a method for analyzing an antigen or antibody labeled with acridinium ester, and an immunoassay kit prepared by the method. Background technique:

[0002] Chemiluminesent Immunoassay (CLIA) is an emerging assay developed by immunoassay technology following enzyme immunoassay (EIA), radioimmunoassay (RIA), fluorescence immunoassay (FIA) and time-resolved fluorescence immunoassay (TRFIA). technology.

[0003] Chemiluminescence technology is a measurement method developed in the past two or three decades. It uses the free energy released by chemical reactions to excite intermediates (commonly used alkaline phosphatase-amantadine), making it return from the excited state to the ground state, When the intermediate returns from the excited state to the ground state, photons of the same energy level are released, and the photons are measured for quantitative analysis. ...

Claims

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