Quantitative determination kit of inhibin A and preparation method of quantitative determination kit

Abstract

The invention discloses a quantitative determination kit of inhibin A. The quantitative determination kit comprises an experimental buffer solution, an enhancement solution, wash concentrate, a marker, a calibration product and a coated plate, wherein the experimental buffer solution comprises calf serum and a first buffer solution at a volume ratio of 0.1-100%; the enhancement solution contains 0.4-0.6ml / L TritonX-100 aqueous solution; the wash concentrate comprises 3-5ml / L Tween-20 and a second buffer solution; the marker is prepared from a monoclonal antibody of the inhibin A and a chelate of lanthanide ions at a ratio of 1:1-1:3m / m; the calibration product comprises freeze-dried powder with six concentrations, wherein the freeze-dried powder is formed by freeze-drying an antigens of the inhibin A and a third buffer solution; the coated plate is a coated plate made of coating liquid, confining liquid and a reaction plate and comprises 0.1-10mug / mL monoclonal antibody of the inhibin A and a fourth buffer solution, and the confining liquid is a fifth buffer solution. The invention also discloses a preparation method of the quantitative determination kit of the inhibin A. According to the kit disclosed by the invention, the defects in chemiluminescence immunoassay and enzyma-linked immumosorbent assay can be avoided, and a determination result is accurate and reliable.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an inhibin A quantitative detection kit and a preparation method thereof. Background technique [0002] Inhibin (Inhibin, INH) is a member of the transforming growth factor β (TGF-β) superfamily, and is a heterodimeric glycoprotein hormone composed of α-subunits and β-subunits linked by disulfide bonds. The β-subunit has two forms, βA and βB, and forms inhibin A (INH A, αβA) and inhibin B (INH B, αβB) with the α-subunit, respectively. [0003] At present, for human inhibin A, the detection kit mainly adopts chemiluminescent immunoassay, and for human inhibin B, the detection kit mainly adopts enzyme-linked immunosorbent reaction. When using chemiluminescence immunoassay detection, the emission intensity of chemiluminescence depends on various environmental factors. In different environmental systems, the curves of emission intensity and time are quite different. Therefore, various e...

AI Technical Summary

Problems solved by technology

When ELISA is used for detection, the reaction steps are cumbersome, the reaction time is long, and the enzyme reaction is easily interfered by various interference factors, and the specificity and sensitivity of the reaction are low.

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