Quantitative determination kit of inhibin A and preparation method of quantitative determination kit

A quantitative detection and inhibin technology, which is applied in the field of inhibin A quantitative detection kit and its preparation, can solve the problems of cumbersome reaction steps, long reaction time, low specificity and sensitivity, etc., and achieve accurate measurement results and avoid defects. Effect

Inactive Publication Date: 2017-05-31
GUANGZHOU FENGHUA BIOENG
View PDF4 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When ELISA is used for detection, the reaction steps are cumbersome, the reaction time is long, and the enzyme reaction is easily interfered by various interference factors, and the specificity and sensitivity of the reaction are low.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Quantitative determination kit of inhibin A and preparation method of quantitative determination kit
  • Quantitative determination kit of inhibin A and preparation method of quantitative determination kit
  • Quantitative determination kit of inhibin A and preparation method of quantitative determination kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0020] The inhibin A quantitative detection kit of the present invention includes an experimental buffer, an enhancement solution, a concentrated washing solution, a marker, a calibrator and a coated plate, and the preparation methods of the above-mentioned parts are as follows.

[0021] Preparation of experimental buffer: add volume to pH 7.8, 50mmol / L Tris-HCl solution containing 5g / L NaCl, 4‰EDTA, 10‰FPC, 6‰rat serum, 6‰thimerosal, 6% eosin Ratio of 50% calf serum, mix well.

[0022] Preparation of enhancement solution: 0.5g / L sodium acetate, 0.003g / Lβ-NTA, 0.024g / L TOPO, 0.8‰ glacial acetic acid, 1‰ absolute ethanol, 0.5ml / L TritonX-100 were prepared into an aqueous solution, prepared The aqueous solution is the enhancement solution. Among them, β-NTA is β-naphthoyltrifluoroacetone, and TOPO is tri-n-octylphosphine oxide.

[0023]Preparation of concentrated lotion: Add 4ml / L Tween-20 to the pH7.8, 50mmol / L Tris-HCl solution containing 22.5g / L NaCl, 0.5ml / L FPC, and 0.08‰...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a quantitative determination kit of inhibin A. The quantitative determination kit comprises an experimental buffer solution, an enhancement solution, wash concentrate, a marker, a calibration product and a coated plate, wherein the experimental buffer solution comprises calf serum and a first buffer solution at a volume ratio of 0.1-100%; the enhancement solution contains 0.4-0.6ml / L TritonX-100 aqueous solution; the wash concentrate comprises 3-5ml / L Tween-20 and a second buffer solution; the marker is prepared from a monoclonal antibody of the inhibin A and a chelate of lanthanide ions at a ratio of 1:1-1:3m / m; the calibration product comprises freeze-dried powder with six concentrations, wherein the freeze-dried powder is formed by freeze-drying an antigens of the inhibin A and a third buffer solution; the coated plate is a coated plate made of coating liquid, confining liquid and a reaction plate and comprises 0.1-10mug / mL monoclonal antibody of the inhibin A and a fourth buffer solution, and the confining liquid is a fifth buffer solution. The invention also discloses a preparation method of the quantitative determination kit of the inhibin A. According to the kit disclosed by the invention, the defects in chemiluminescence immunoassay and enzyma-linked immumosorbent assay can be avoided, and a determination result is accurate and reliable.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an inhibin A quantitative detection kit and a preparation method thereof. Background technique [0002] Inhibin (Inhibin, INH) is a member of the transforming growth factor β (TGF-β) superfamily, and is a heterodimeric glycoprotein hormone composed of α-subunits and β-subunits linked by disulfide bonds. The β-subunit has two forms, βA and βB, and forms inhibin A (INH A, αβA) and inhibin B (INH B, αβB) with the α-subunit, respectively. [0003] At present, for human inhibin A, the detection kit mainly adopts chemiluminescent immunoassay, and for human inhibin B, the detection kit mainly adopts enzyme-linked immunosorbent reaction. When using chemiluminescence immunoassay detection, the emission intensity of chemiluminescence depends on various environmental factors. In different environmental systems, the curves of emission intensity and time are quite different. Therefore, various e...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/74G01N33/531
CPCG01N33/74G01N33/531G01N2333/655
Inventor 冯健明徐倩梁琪芬陈龙
Owner GUANGZHOU FENGHUA BIOENG
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap