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Preparation method of fluorescence gold nano clusters with stable chicken ovalbumin

A technology of fluorescent gold nanometer and albumin, which is applied in the fields of nanotechnology, nanotechnology, nanotechnology for materials and surface science, and can solve the problems of poor singleness and wide volume distribution.

Active Publication Date: 2013-12-25
HUNAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most of the volumes of polymers and small molecule-stabilized noble metal nanoclusters have polydispersity, that is, their volume distribution is wide and their uniformity is poor.

Method used

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  • Preparation method of fluorescence gold nano clusters with stable chicken ovalbumin
  • Preparation method of fluorescence gold nano clusters with stable chicken ovalbumin
  • Preparation method of fluorescence gold nano clusters with stable chicken ovalbumin

Examples

Experimental program
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Effect test

Embodiment 1

[0019] Weigh 20 mg of chicken ovalbumin, add 20 mL of deionized water to dissolve, and prepare a 1 mg / mL protein solution. Take 0.2mL of 0.05M chloroauric acid solution, add 20mL of chicken ovalbumin solution with a concentration of 1mg / mL, mix well; use NaOH solution with a concentration of 1M to adjust the pH of the mixed solution to 12, and place it in a microwave reactor React for 110 s, take out and cool naturally to obtain a red-emitting gold nanocluster solution. figure 1 is the fluorescence emission spectrum of the prepared gold nanoclusters (excitation wavelength 520nm).

Embodiment 2

[0021] Weigh 60 mg of chicken ovalbumin, add 20 mL of deionized water to dissolve, and prepare a 3 mg / mL protein solution. Take 0.2mL of 0.05M chloroauric acid solution, add 20mL of chicken ovalbumin solution with a concentration of 3mg / mL, and mix well; use NaOH solution with a concentration of 1M to adjust the pH of the mixed solution to 12, and place it in a microwave reactor React in middle temperature for 30s, take out and cool naturally to obtain a red-emitting gold nanocluster solution. figure 2 is the fluorescence emission spectrum of the prepared gold nanoclusters (excitation wavelength 520nm).

Embodiment 3

[0023] Weigh 100mg chicken ovalbumin, add 20mL deionized water to dissolve, and prepare a 5mg / mL protein solution. Take 0.2mL of 0.05M chloroauric acid solution, add 20mL of chicken ovalbumin solution with a concentration of 3mg / mL, and mix well; use NaOH solution with a concentration of 1M to adjust the pH of the mixed solution to 12, and place it in a microwave reactor React in medium for 25s, take out and cool naturally to obtain a red-emitting gold nanocluster solution. image 3 is the fluorescence emission spectrum of the prepared gold nanoclusters (excitation wavelength 520nm).

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Abstract

The invention relates to a preparation method of fluorescence gold nano clusters with stable chicken ovalbumin. The method includes: dissolving commercially available chicken ovalbumin in deionized water to prepare solution 0.5-10mg / mL in concentration; mixing the solution with chloroauric acid aqueous solution to obtain mixed solution, with certain mole ratio, of the chicken ovalbumin and the chloroauric acid aqueous solution; adjusting pH of the mixed solution to 11.5-13, allowing for microwave reaction for 15-20s, and naturally cooling to room temperature to obtain fluorescence gold nano clusters emitting red light. The fluorescence gold nano clusters prepared by the method is applicable to the bio-metical field and the sensor field.

Description

technical field [0001] The invention relates to a method for preparing fluorescent gold nanoclusters applicable to biomedicine and heavy metal ion sensing. Background technique [0002] Noble metal nanoclusters not only have strong fluorescence efficiency, small physical size (less than 1 nm), but also have better biocompatibility and photostability than popular luminescent materials quantum dots. Therefore, noble metal nanoclusters have good application prospects, so they have attracted the attention of many scientists. At present, the research on the preparation and application of noble metal nanoclusters has attracted many researchers at home and abroad. As reported, polymers, small molecules, etc. are used as stabilizers or templates, and NaBH 4 Noble metal nanoclusters prepared by methods such as reducing noble metal ions or etching large noble metal nanocluster particles by electrochemical, hydrothermal, light, ultrasonic and other methods. However, most of the volu...

Claims

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Application Information

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IPC IPC(8): B22F9/24C09K11/58B82Y30/00B82Y40/00
Inventor 廖博龙鹏陈丽娟曾文南肖琰
Owner HUNAN UNIV OF SCI & TECH
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