The invention discloses a method for preparing
chondroitin sulfate. The method comprises the following steps: adding water to immerse
cartilage, adjusting the pH value to be 8-9 with alkali, and carrying out heat preservation at 55-60 DEG for 4-6 hours; adding 2709
alkaline protease, stirring for 6-8 hours, heating until boiling, and filtering to obtain
chondroitin sulfate liquid; adding
sodium chloride to the
chondroitin sulfate liquid, adding ethyl
alcohol which is 2-3 times of volume of the liquid, standing for 6 hours, and filtering to obtain
chondroitin sulfate sediments; adding water to the
chondroitin sulfate sediments for dissolving, simultaneously adding
sodium chloride to water, and adjusting pH value to be 10-12; adding
hydrogen peroxide which is 1%-3% of weight of the liquid, oxidizing for 6 hours, and filtering to obtain filtrate; adjusting the pH value of the
chondroitin sulfate filtrate to 5-6, adding ethyl
alcohol which is 4-6 times of volume of the filtrate, and participating for the second time, so as to obtain chondroitin sulfate sediments; pouring the chondroitin sulfate sediments into a
dewatering tank with an emulsifier; pumping ethyl
alcohol which is 3-5 times of volume of the chondroitin sulfate sediments into the
dewatering tank, carrying out suction
filtration, collecting sediments, and
drying to obtain a chondroitin sulfate product. The chondroitin sulfate obtained by the method is loose in texture, small in particles, easy to dewater and dry, low in
energy consumption, very low in
organic solvent residue and short in
production cycle.