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A technology of chondroitin sulfate and cartilage, applied in the biological field, can solve problems such as long precipitation time, easy dehydration, and affecting product production efficiency, and achieve the effect of less organic solvent residue, easy dehydration, and fine particles
Inactive Publication Date: 2015-03-25
QINGDAO JIULONG BIO PHARMA
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[0009] It is easy to dehydrate and dry, does not need to be crushed, and the product has good color and stable quality. The content of chondroitin sulfate is more than
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Embodiment 1
[0020] Embodiment 1: prepare chondroitin sulfate according to the following steps
[0021] (1) Alkaline extraction and enzymolysis: first, 100Kg of cartilage raw material is submerged in water, adjust pH to 8 with alkali, heat up to 55°C, and keep warm for 6 hours; then, add 1Kg2709 alkaline protease, keep pH 8, and stir at 55°C for 8 hours hour, be warming up to boiling for 12 minutes, filter to obtain chondroitin sulfate solution 500L;
[0022] (2) Alcohol precipitation: the chondroitin sulfate solution obtained in step (1) is down to room temperature, and under stirring, add 5Kg sodium chloride to the solution, so that the solution contains a sodium chloride weight concentration of 1%, and then add the solution 1000L of alcohol with a weight concentration of 2 times the volume of more than 90%, let it stand still for more than 6 hours, and filter to obtain the chondroitin sulfate precipitation;
[0023] (3) Oxidation: Add the chondroitin sulfate precipitate obtained in st...
Embodiment 2
[0026] Embodiment 2: prepare chondroitin sulfate according to the following steps
[0027] (1) Alkaline extraction and enzymatic hydrolysis: first, 100 Kg of cartilage raw material is submerged in water, adjust the pH to 8.5 with alkali, heat up to 58°C, and keep warm for 5 hours; then, add 2Kg2709 alkaline protease, keep pH 8.5, and keep warm at 58°C C stirred for 7 hours, was warming up to boiling for 15 minutes, filtered to obtain chondroitin sulfate solution 500L;
[0028] (2) Alcohol precipitation: the chondroitin sulfate solution obtained in step (1) is lowered to room temperature, and under stirring, 13Kg sodium chloride is added to the solution to make the solution contain a sodium chloride weight concentration of 2.5%, and then add the solution 2.5 times the volume of alcohol 1250L with a weight concentration of more than 90%, let it stand for more than 6 hours, and filter to obtain the chondroitin sulfate precipitate;
[0029] (3) Oxidation: Add the chondroitin sul...
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Abstract
The invention discloses a method for preparing chondroitin sulfate. The method comprises the following steps: adding water to immerse cartilage, adjusting the pH value to be 8-9 with alkali, and carrying out heat preservation at 55-60 DEG for 4-6 hours; adding 2709 alkaline protease, stirring for 6-8 hours, heating until boiling, and filtering to obtain chondroitin sulfate liquid; adding sodium chloride to the chondroitin sulfate liquid, adding ethyl alcohol which is 2-3 times of volume of the liquid, standing for 6 hours, and filtering to obtain chondroitin sulfate sediments; adding water to the chondroitin sulfate sediments for dissolving, simultaneously adding sodium chloride to water, and adjusting pH value to be 10-12; adding hydrogen peroxide which is 1%-3% of weight of the liquid, oxidizing for 6 hours, and filtering to obtain filtrate; adjusting the pH value of the chondroitin sulfate filtrate to 5-6, adding ethyl alcohol which is 4-6 times of volume of the filtrate, and participating for the second time, so as to obtain chondroitin sulfate sediments; pouring the chondroitin sulfate sediments into a dewatering tank with an emulsifier; pumping ethyl alcohol which is 3-5 times of volume of the chondroitin sulfate sediments into the dewatering tank, carrying out suction filtration, collecting sediments, and drying to obtain a chondroitin sulfate product. The chondroitin sulfate obtained by the method is loose in texture, small in particles, easy to dewater and dry, low in energy consumption, very low in organic solvent residue and short in production cycle.
Description
technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation method of chondroitin sulfate. Background technique [0002] Chondroitin sulfate, English name chondroitin sulfate, is a polymeric acidic mucopolysaccharide made from the cartilage of animals (pigs, cattle, horses, sheep, chickens, sharks, etc.), and its molecular structure is from β-D-glucosaldehyde A polysaccharide chain consisting of repeating disaccharide units formed from β1 → 3 glycosidic bonds through acid and N-acetylgalactosamine. It is a white or yellowish powder with strong water absorption, easily soluble in water to form a viscous liquid, insoluble in organic solvents such as alcohol, acetone, ether and chloroform, and its salts are relatively stable to heat. [0003] At present, chondroitin sulfate usually adopts alkali-enzymatic extraction method, which mainly includes the following steps: [0004] 1) Alkaline extraction and enzymolysis: the...
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