Method for polyploidy embedding induction of Jiangxi Qianshan Colocasia esulenla Schott
A technology of red bud taro and polyploidy, which can be used in horticultural methods, botanical equipment and methods, plant genetic improvement and other directions, and can solve problems such as inability to meet demand.
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[0036] (1) Take a single bud of Jiangxi Yanshan red bud taro and soak it for 48 hours in MS culture medium a containing colchicine, chitosan oligosaccharides and graphene quantum dots;
[0037] (2) Step (1) Soak the soaked Jiangxi Yanshan red bud taro single bud with MS culture medium b containing colchicine, chitosan oligosaccharides and carbon nanotubes for 48 hours;
[0038] (3) mixing the Jiangxi Yanshan red bud taro single bud soaked in step (2) with calcium ion-free modified MS culture medium c containing sodium alginate and sucrose to obtain a mixed solution;
[0039] (4) Drop the mixed solution of step (3) into the MS culture solution d containing colchicine, chitosan oligosaccharide, graphene quantum dots, carbon nanotubes, calcium chloride and sucrose for embedding to obtain single buds of taro embedding beads;
[0040] (5) Inoculate the red bud taro single-bud embedding beads prepared in step (4) in MS solid medium for culture, the culture conditions are light time...
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