Method for polyploidy embedding induction of Jiangxi Qianshan Colocasia esulenla Schott

A technology of red bud taro and polyploidy, which can be used in horticultural methods, botanical equipment and methods, plant genetic improvement and other directions, and can solve problems such as inability to meet demand.

Active Publication Date: 2020-09-11
SHANGRAO NORMAL UNIV
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  • Description
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Problems solved by technology

However, its induction rate is only 20.5%, w

Method used

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  • Method for polyploidy embedding induction of Jiangxi Qianshan Colocasia esulenla Schott
  • Method for polyploidy embedding induction of Jiangxi Qianshan Colocasia esulenla Schott
  • Method for polyploidy embedding induction of Jiangxi Qianshan Colocasia esulenla Schott

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Embodiment 1

[0036] (1) Take a single bud of Jiangxi Yanshan red bud taro and soak it for 48 hours in MS culture medium a containing colchicine, chitosan oligosaccharides and graphene quantum dots;

[0037] (2) Step (1) Soak the soaked Jiangxi Yanshan red bud taro single bud with MS culture medium b containing colchicine, chitosan oligosaccharides and carbon nanotubes for 48 hours;

[0038] (3) mixing the Jiangxi Yanshan red bud taro single bud soaked in step (2) with calcium ion-free modified MS culture medium c containing sodium alginate and sucrose to obtain a mixed solution;

[0039] (4) Drop the mixed solution of step (3) into the MS culture solution d containing colchicine, chitosan oligosaccharide, graphene quantum dots, carbon nanotubes, calcium chloride and sucrose for embedding to obtain single buds of taro embedding beads;

[0040] (5) Inoculate the red bud taro single-bud embedding beads prepared in step (4) in MS solid medium for culture, the culture conditions are light time...

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Abstract

The invention discloses a method for polyploidy embedding induction of Jiangxi Qianshan Colocasia esulenla Schott. Single buds of Colocasia esulenla Schott are soaked in a culture solution containingcolchicine, chitosan oligosaccharide and graphene quantum dots; the soaked single buds are soaked in a culture solution containing colchicine, chitosan oligosaccharide and CNT; the soaked single budsare mixed with a calcium ion free improved culture solution containing sodium alginate and sucrose, and a mixture solution is obtained; the mixture solution is dripped into a culture solution containing the colchicine, chitosan oligosaccharide, graphene quantum dots, CNT, calcium chloride and sucrose for embedding; an embedded plant is inoculated in a solid culture medium for culture; Colocasia esulenla Schott single buds with variation are separated and inoculated into the solid culture medium for 3-4 times of subculture, and the polyploidy multiple shoots of Jiangxi Qianshan Colocasia esulenla Schott are obtained; and when the multiple shoots grow to 2-3 cm, the single buds are cut off and transferred to a rooting medium for culture, so that polyploidy test tube plantlets of Jiangxi Qianshan Colocasia esulenla Schott are obtained. The Colocasia esulenla Schott polyploidy embedding induction frequency reaches up to 86.4%.

Description

technical field [0001] The invention relates to the technical field of polyploid induction, in particular to a method for polyploid induction of Jiangxi Yanshan red bud taro polyploid. Background technique [0002] Jiangxi Yanshan red bud taro has bright skin color, red terminal buds, tender meat, easy to boil but not mushy, rich nutrition, contains a variety of trace elements, is a treasure among taro, and has always been a hot commodity in domestic and foreign markets. It is the flagship product of Qianshan County. At present, with the continuous development of production, the accumulation of viruses in asexual reproduction of plants is gradually increasing, and the red bud taro varieties in Jiangxi Yanshan generally suffer from premature aging, serious diseases, and decline in yield and quality. Therefore, the selection and breeding of fine varieties has become an urgent problem to be solved in the production of red bud taro in Qianshan, Jiangxi. Polyploids generally ha...

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Application Information

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IPC IPC(8): A01H4/00A01H1/08
CPCA01H4/008A01H4/001A01H1/08
Inventor 尹明华魏杰洪森荣
Owner SHANGRAO NORMAL UNIV
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