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Method for embedding, transplanting and domesticating Huai-yu Mountain alpine potato stems with buds

A technology of potato with buds, which is applied in the field of transplanting and domestication, can solve the problems of low survival rate and achieve the effect of easy operation and factory operation

Active Publication Date: 2020-09-29
SHANGRAO NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Huaiyu Mountain alpine potatoes are only suitable for planting in high-altitude alpine areas. Under long-term adversity stress, Huaiyu Mountain alpine potatoes have formed a series of strategies to adapt to the alpine environment. Once Huaiyu Mountain alpine potatoes are moved to low-altitude plains Planting and domestication will break the original adversity adaptation mechanism and produce mutations. At the same time, the survival rate of domesticated Huaiyu Mountain alpine potato test-tube seedlings is relatively low.
And this has also become a bottleneck problem restricting the cultivation of alpine potatoes in Huaiyu Mountain

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Prepare calcium ion-free MS culture solution a: add sodium alginate and sucrose to make the concentration of sodium alginate 3% (w / v) and the concentration of sucrose 0.2mol / L;

[0029] MS culture medium b: chitosan oligosaccharide concentration 0.05g / L, graphene quantum dot concentration 0.03g / L, carbon nanotube concentration 0.03g / L, CaCl 2 Concentration 0.1mol / L and sucrose concentration 0.2mol / L;

[0030] MS culture medium c: chitosan oligosaccharide concentration 0.05g / L, graphene quantum dot concentration 0.03g / L;

[0031] MS culture solution d: chitosan oligosaccharide concentration 0.05g / L, carbon nanotube concentration 0.03g / L;

[0032] (1) Under aseptic room temperature conditions, mix the 0.5 cm section of budded stems of Huaiyu Mountain alpine potatoes with the improved MS culture medium a without calcium ions to obtain a mixed solution. The amount of culture medium a: depends on the amount of budded stems, Generally, a stem section with buds is 1-2mL;

...

Embodiment 2

[0041] With embodiment 1, difference is:

[0042] MS culture medium b: chitosan oligosaccharide concentration 0.05g / L, graphene quantum dot concentration 0.05g / L, carbon nanotube concentration 0.05g / L, CaCl 2 Concentration 0.1mol / L and sucrose concentration 0.2mol / L;

[0043] MS culture medium c: chitosan oligosaccharide concentration 0.05g / L, graphene quantum dot concentration 0.05g / L;

[0044] MS culture medium d: the concentration of chitosan oligosaccharides is 0.05g / L, and the concentration of carbon nanotubes is 0.05g / L;

[0045] The results showed that the state of the domesticated Huaiyu Mountain alpine potato was more robust than the transplanted test-tube seedlings; and 100% of them grew new leaves and survived, and the survival rate was 100%.

Embodiment 3

[0047] With embodiment 1, difference is:

[0048] MS culture medium b: chitosan oligosaccharide concentration 0.05g / L, graphene quantum dot concentration 0.04g / L, carbon nanotube concentration 0.04g / L, CaCl 2 Concentration 0.1mol / L and sucrose concentration 0.2mol / L;

[0049] MS culture medium c: chitosan oligosaccharide concentration 0.05g / L, graphene quantum dot concentration 0.04g / L;

[0050] MS culture solution d: chitosan oligosaccharide concentration 0.05g / L, carbon nanotube concentration 0.04g / L;

[0051] The results showed that the state of the domesticated Huaiyu Mountain alpine potato was more robust than the transplanted test-tube seedlings; and 100% of them grew new leaves and survived, and the survival rate was 100%.

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Abstract

The invention discloses a method for embedding, transplanting and domesticating Huai-yu Mountain alpine potato stems with buds. The method comprises the following steps: mixing the Huai-yu Mountain alpine potato stems with buds with a calcium-free ion improved MS culture solution (a) containing sodium alginate and sucrose to obtain a mixed solution; dropwise adding the mixed solution into an MS culture solution (b) containing chitosan oligosaccharide, graphene quantum dots, carbon nanotubes, calcium chloride and sucrose and carrying out embedding to obtain embedded balls of the Huai-yu Mountain alpine potato stems with buds; soaking the prepared embedded balls with the MS culture solution containing the chitosan oligosaccharide and the graphene quantum dots; soaking the soaked embedded balls with the MS culture solution containing the chitosan oligosaccharide and the carbon nanotubes; transplanting the embedded balls into a matrix and irrigating the embedded balls with the MS culture solution containing the chitosan oligosaccharide and the carbon nanotubes; after irrigating for 7 days, irrigating the embedded balls with the MS culture solution containing the chitosan oligosaccharide and the graphene quantum dots; after the stems with buds grow into complete plants, finishing domestication and transplanting the complete plants into a field for breeding. By adopting the method disclosed by the invention, the transplanting and domesticating survival rate of test-tube plantlets of Huai-yu Mountain alpine potatoes can be 98% or above.

Description

technical field [0001] The invention relates to the technical field of transplanting and acclimation, and relates to a method for embedding, transplanting and acclimatization of Huaiyu Mountain alpine potato with bud stem segments. Background technique [0002] Native to the Andes in South America, the potato is an important food and vegetable crop and the most important tuber crop in the world. Potatoes contain a large amount of trace elements, proteins, amino acids, B and C vitamins, and have the reputation of "second bread", "king of nutrition", "underground apple" and so on. Due to the high yield, strong adaptability, easy cultivation, and low water consumption of the growth cycle, potatoes can be supplied throughout the year. Therefore, potatoes can also produce high economic benefits in remote water-scarce areas. It is an industry that helps my country's poverty alleviation. In 2015, with the launch of the potato staple food strategy, potatoes have become China's four...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G31/00A01G24/15
CPCA01H4/00A01H4/001A01G31/00A01G24/15
Inventor 洪森荣曾晓健尹明华
Owner SHANGRAO NORMAL UNIV
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