Preparation method of chondroitin sulfate

Abstract

The invention discloses a preparation method of chondroitin sulfate, which comprises the following steps of: adding water until cartilage is immersed; adjusting the pH value to be 8-9 with alkali and preserving heat at a temperature of 55-60DEG C for 4-6h; adding 2709 alkali protease and stirring for 6-8h, raising the temperature to boiling and filtering to obtain a chondroitin sulfate solution; adding sodium chloride to the chondroitin sulfate solution; adding alcohol of which the volume is 2-3 times of that of the solution, standing for 6h and filtering to obtain chondroitin sulfate precipitate; adding the chondroitin sulfate precipitate into water and dissolving; meanwhile, adding sodium chloride to the water, adjusting the pH value to be 10-12, adding hydrogen peroxide which accounts for 1-3 percent of the weight of the solution, oxidizing for 6h and filtering to obtain filtrate; adjusting the pH value of the chondroitin sulfate filtrate to be 5-6, adding alcohol of which the volume is 4-6 times of filtrate for secondary precipitation to obtain the chondroitin sulfate precipitate; pouring the secondary chondroitin sulfate precipitate into a dehydration tank provided with an emulsifying machine; pumping alcohol of which the volume is 3-5 times that of the chondroitin sulfate precipitate into the dehydration tank; and carrying out suction filtering, collecting the precipitate and drying to obtain a chondroitin sulfate finished product. The chondroitin sulfate obtained by the preparation method disclosed by the invention has the advantages of loose texture, fine particles, easiness of dehydration and drying, low energy consumption, extremely-low residual organic solvent and short production period.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation method of chondroitin sulfate. Background technique [0002] Chondroitin sulfate, English name chondroitin sulfate, is a kind of polymeric acidic mucopolysaccharide made from the cartilage of animals (pigs, cattle, horses, sheep, chickens, sharks, etc.), and its molecular structure is β-D-glucuronic acid It is a polysaccharide chain composed of repeating disaccharide units formed by β1→3 glycosidic bonds with N-acetylgalactosamine. It is a white or slightly yellow powder with strong water absorption, easily soluble in water to form a viscous liquid, insoluble in organic solvents such as alcohol, acetone, ether and chloroform, and its salts are relatively stable to heat. [0003] At present, chondroitin sulfate is usually extracted by alkali-enzymatic hydrolysis, which mainly includes the following steps: [0004] 1) Alkaline extraction and enzymatic hydrol...

AI Technical Summary

Problems solved by technology

[0008] Because the chondroitin sulfate solution is relatively viscous, the traditional method is to add alcohol to the chondroitin sulfate solution to precipitate the chondroitin sulfate crystals during alcohol precipitation. During the dehydration and drying process, the organic solvent contained in the crystal is not easy to volatilize due to its large particle shape and hard and dense particle shape, resulting in high solvent residue in the product, ≥ 2%, which affects product quality and cannot meet customer requirements

Secondly, due to the viscous sedimentation and agglomeration, operations such as dehydration, drying, and crushing become difficult and waste energy

An invention patent application submitted by the applicant previously disclosed a method for producing chondroitin sulfate by reverse precipitation. The chondroitin sulfate obtained by precipitation is crisp and loose, easy to dehydrate and dry, does not need to be crushed, and the color of the product is good , the quality is stable, the content of chondroitin sulfate is above 98%, and the residual organic solvent is less than 0.2%, but the precipitation time of this method is long, which affects the production efficiency of the product