In-bottle rooting method for salt tolerance ulmus pumila tissue culture seedling

A technology for tissue culture seedlings and salt tolerance is applied in the field of in-bottle rooting of salt-tolerant white elm tissue culture seedlings. , the effect of improving the rooting rate

Active Publication Date: 2012-10-17
上海菁艺生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] White elm is generally propagated by seeds, but the good characteristics of good varieties will decline after sexual reproduction. In order to better maintain the good characteristics of the variety, asexual reproduction must be carried out, and the good characteristics of the variety must be maintained by means of graft

Method used

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  • In-bottle rooting method for salt tolerance ulmus pumila tissue culture seedling
  • In-bottle rooting method for salt tolerance ulmus pumila tissue culture seedling
  • In-bottle rooting method for salt tolerance ulmus pumila tissue culture seedling

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Select the salt-tolerant Ulmus elm tissue culture seedlings that are subcultured, and the selection standard is a single elm seedling with a height of 2-3.5cm, strong, and stretched leaves;

[0039] Inoculate the selected subcultured salt-tolerant Ulmus alba tissue culture seedlings on the WPM rooting medium;

[0040] Wherein, the WPM rooting medium is a 1 / 2 WPM rooting medium that is reduced by 1 / 2 with the macroelements in the WPM basic medium.

[0041] The growth hormone is indolebutyric acid (IBA), and its final concentration is 0.05mg / L; the cytokinin is naphthaleneacetic acid (NAA), and its final concentration is 0.05mg / L; the exogenous added The substances are activated carbon (AC) and white sugar, the final concentration of the activated carbon (AC) is 0.2g / L, and the final concentration of the white sugar is 20g / L.

[0042] The pH value of the WPM rooting medium is 5.8.

[0043] After 25 days of inoculation, count the rooting rate, rooting rate=number of root...

Embodiment 2

[0045] Select the salt-tolerant Ulmus elm tissue culture seedlings that are subcultured, and the selection standard is a single elm seedling with a height of 2-3.5cm, strong, and stretched leaves;

[0046] Inoculate the selected subcultured salt-tolerant Ulmus alba tissue culture seedlings on the WPM rooting medium;

[0047] Wherein, the WPM rooting medium is a 1 / 2 WPM rooting medium that is reduced by 1 / 2 with the macroelements in the WPM basic medium.

[0048] The growth hormone is indolebutyric acid (IBA), and its final concentration is 0.2mg / L; the cytokinin is naphthaleneacetic acid (NAA), and its final concentration is 0.1mg / L; the exogenous added The substances are activated carbon (AC) and white sugar, the final concentration of the activated carbon (AC) is 0.5g / L, and the final concentration of the white sugar is 30g / L.

[0049] The pH value of the WPM rooting medium is 5.85.

[0050] After 25 days of inoculation, count the rooting rate, rooting rate=number of roote...

Embodiment 3

[0052] Select the salt-tolerant Ulmus elm tissue culture seedlings that are subcultured, and the selection standard is a single elm seedling with a height of 2-3.5cm, strong, and stretched leaves;

[0053] Inoculate the selected subcultured salt-tolerant Ulmus alba tissue culture seedlings on the WPM rooting medium;

[0054] Wherein, the WPM rooting medium is a 1 / 2 WPM rooting medium that is reduced by 1 / 2 of the macroelements in the WPM basic medium.

[0055] The growth hormone is indolebutyric acid (IBA), and its final concentration is 0.3mg / L; the cytokinin is naphthaleneacetic acid (NAA), and its final concentration is 0.2mg / L; the exogenous added The substances are activated carbon (AC) and white sugar, the final concentration of the activated carbon (AC) is 2g / L, and the final concentration of the white sugar is 40g / L.

[0056] The pH value of the WPM rooting medium is 5.9.

[0057] After 25 days of inoculation, count the rooting rate, rooting rate=number of rooted pla...

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Abstract

The purpose of the present invention is to disclose an in-bottle rooting method for a salt tolerance ulmus pumila tissue culture seedling. The method comprises the following steps: (1) selecting a secondary seedling, wherein a secondary culture salt tolerance ulmus pumila tissue culture seedling is selected, the height of the seedling is 2-3.5 cm, and the robust individual ulmus pumila seedling having extending leaves is selected; and (2) inoculating the selected secondary culture salt tolerance ulmus pumila tissue culture seedling onto a WPM rooting medium, wherein the WPM rooting medium is added with growth hormone, cytokinin and exogenous additives. According to the present invention, a medium formula having a high rooting rate is adopted so as to effectively solve the rooting problem of the salt tolerance ulmus pumila tissue culture seedling, improve the rooting rate of the salt tolerance ulmus pumila tissue culture seedling, reduce the cost, accelerate the factory production of the salt tolerance ulmus pumila, provide high quality salt tolerance ulmus pumila seedlings for nursery stock markets, and achieve the purpose of the present invention, wherein the rooting rate of the salt tolerance ulmus pumila tissue culture seedling can be up to 95%.

Description

technical field [0001] The invention relates to a method for rooting in a bottle for cultivating seedlings, in particular to a method for rooting in a bottle for salt-tolerant elm tissue cultured seedlings. Background technique [0002] White elm, also known as elm, is a deciduous tree in the family Ulmaceae, with a wide distribution and mainly produced in the northern temperate zone. White elm is light-loving, drought-tolerant, cold-resistant, and barren-resistant. It does not choose soil and has strong adaptability. It is mostly used for afforestation. White elm wood is wear-resistant and corrosion-resistant, and is an excellent wood for shipbuilding, construction, interior decoration floors, and furniture. The bark fiber is tough and can be used as raw material for rayon and papermaking. The leaves contain starch and protein and can be used as feed. The skin, leaves and fruit can be used as medicine, and the seeds can be used to extract oil, which is the raw material o...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 辛全伟幕宗昭邵云华章振华董举文高畅
Owner 上海菁艺生物科技有限公司
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