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Preparation method of acellular matrix gels and acellular matrix gels

An acellular matrix and gel technology, applied in the field of acellular matrix gel and the preparation of acellular matrix gel, can solve the problems of difficult patient wounds, easy deformation, single biological function, etc., to promote regeneration and repair, Good biological function, the effect of prolonging the degradation time

Active Publication Date: 2015-12-23
SUN YAT SEN UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0006] In order to overcome the shortcomings of traditional natural polymer gels with single components and single biological functions, and to overcome the shortcomings of traditional acellular matrix materials such as easy deformation, large batch differences, poor processability, and difficulty in fully matching with patients' wounds, the present invention, Provided is a method for preparing an acellular matrix gel and the acellular matrix gel thereof. The preparation method of the present invention is a technique in which the acellular matrix is ​​subjected to subsequent processing of powdering and digestion, and is gently formed into a gel under normal physiological conditions of the human body. Gelation technology can be used in both injectable gels and processable gels, and the formed gel has a microscopic nanofibrous structure, which has a positive effect on regulating the behavior of cells

Method used

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  • Preparation method of acellular matrix gels and acellular matrix gels
  • Preparation method of acellular matrix gels and acellular matrix gels
  • Preparation method of acellular matrix gels and acellular matrix gels

Examples

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example 1

[0038] A specific preparation method of acellular matrix gel is as follows:

[0039](1) Take fresh peripheral nerves from pigs, cut off the fat tissue and part of the epineurium on the surface, shake in distilled water, and rinse for 6 hours. Rinse 3 times, then put into 4% sodium deoxycholate aqueous solution and shake at room temperature for 24 hours, and finally rinse 3 times in distilled water, such a cycle is extraction once, a total of 2 cycles; The cells in the nerves are washed away with DNA and other substances that may cause an immune response to obtain decellularized pig nerves.

[0040] (2) Freeze-dry the decellularized porcine nerve, and grind the decellularized porcine nerve into powder with a pulverizer. The diameter of the powder is about 200 μm, and the microstructure is as follows: figure 1 As shown; Add 1 part of pepsin and 10 parts of decellularized nerve into 100 parts of 0.01mol / L Hcl solution, keep stirring at room temperature (25°C) for 24 hours, and u...

example 2

[0044] A specific preparation method of the acellular matrix gel of the present invention is as follows:

[0045] (1) Take the fresh peripheral tendons of pigs, cut off the fat tissue and part of the adventitia on the surface, shake and rinse in distilled water for 6 hours, put the nerves in 3% Triton-X100 aqueous solution for 12 hours when extracting, and then put them in distilled water Rinse 3 times, then put into 4% sodium deoxycholate aqueous solution and shake at room temperature for 24 hours, and finally rinse 3 times in distilled water, such a cycle is extraction once, a total of 2 cycles; Cells in the nerves and washed away DNA and other substances that may cause an immune response to obtain decellularized pig tendon;

[0046] (2) Freeze-dry the decellularized porcine tendon, and then grind the decellularized porcine tendon into powder with a pulverizer. The diameter of the powder is about 200 μm. Add 1 part of pepsin and 10 parts of decellularized tendon to 100 parts...

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Abstract

The invention discloses a preparation method of acellular matrix gels, which is implemented through that natural tissues or organs are treated by using a decellation technology, the obtained object is subjected to subsequent chalking and enzymatic digestion treatment so as to obtain an acellular matrix solution, and the acellular matrix solution can be prepared into acellular matrix gels with various shapes and properties under mild conditions. Acellular matrix gels prepared by using the preparation method disclosed by the invention can be used in injectable gels and can be processed and formed into gels, the formed gel has a nano fiber microstructure, and the microstructure has a positive effect on regulating the behaviors of cells.

Description

technical field [0001] The invention relates to the technical field of biomaterials, in particular to a method for preparing an acellular matrix gel and the acellular matrix gel. Background technique [0002] Hydrogels are increasingly used in tissue engineering due to their flexibility, porous properties, shape changeability and adjustable strength. Although the synthetic polymer gels have good properties and can be adjusted, they are all biologically inert, and cannot provide the biological function of promoting the growth of cells and tissues in tissue engineering applications. Therefore, in tissue engineering applications, natural polymers that can provide biological activity will be selected as the main component or active component. [0003] Natural polymer hydrogels often use agarose, collagen, gelatin, hyaluronic acid, chitosan, fibrin, etc., and can be divided into physical and chemical gels according to their forming methods. Physical gelation is mainly controlle...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/58A61L27/52
Inventor 全大萍杨伟红刘晟杨习锋
Owner SUN YAT SEN UNIV
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