21 results about "Skeletal muscle damage" patented technology

The invention discloses an application of ganoderma lucidum spore oil in preparation of a medicine or health food for improving sarcopenia. Ganoderma lucidum spore oil can effectively improve weight loss and muscle hypofunction induced by glucocorticoid, and improve the holding power and exercise tolerance level of the body; meanwhile, the skeletal muscle mass and the muscle fiber cross sectional area can be increased, and the skeletal muscle tissue morphological structure is improved; in addition, the ganoderma lucidum spore oil can improve the oxidation resistance of the body and skeletal muscle and reduce the oxidative stress level, so that the skeletal muscle injury related to aging is relieved on the whole. Therefore, the ganoderma lucidum spore oil has a definite application value in the aspect of improving the skeletal muscle function.

The invention relates to application of a CSPR3 gene in promoting skeletal muscle regeneration. The invention provides a system of a transplantation material for promoting skeletal muscle injury repair and application of the system by utilizing a CSPR3 gene, and reveals that the CSRP3 gene participates in a repair process after skeletal muscle injury. The CSRP3 is knocked down in the skeletal muscle, so that the regeneration capacity of the muscle is destroyed; on the contrary, overexpression of the CSRP3 in the skeletal muscle accelerates the repair process of the skeletal muscle after injury. The skeletal muscle injury repair process can be obviously promoted through an allograft strategy, and the repair time is shortened. The invention provides a direction for promoting the development of a clinical treatment strategy for human skeletal muscle injury.

The invention provides application of an Sdc1 overexpression vector in preparation of a medicine for promoting skeletal muscle injury repair, and belongs to the technical field of biomedicine. The invention discloses application of an Sdc1 overexpression vector in preparation of a medicine for promoting skeletal muscle injury repair. Experiments prove that the overexpression of the Sdc1 gene can effectively promote the regeneration of the skeletal muscle of a mouse, and the knockout of the Sdc1 gene can effectively inhibit the proliferation of satellite cells of the skeletal muscle of the mouse so as to hinder the regeneration and repair of the skeletal muscle, so that the Sdc1 overexpression vector can be used for preparing a medicine for treating and promoting the repair of skeletal muscle injury; good application prospects are realized on promotion of skeletal muscle injury repair.

The invention discloses an interpretable machine learning method for predicting multi-organ injuries of diquat poisoning patients, and the method comprises the steps: constructing and verifying a prediction system of eight kinds of organ injuries through a clinical database; toxic encephalopathy, myocardial injury, acute kidney injury, circulatory failure, skeletal muscle injury, liver injury, lung injury and gastrointestinal tract injury are covered. According to the method, early-stage efficient prediction of multi-organ injury is achieved, deep insight is provided for potential biological mechanisms of organ injury, clinical formulation of individualized intervention strategies is facilitated, the prognosis management level of diquat poisoning patients is expected to be remarkably improved, and the incidence rate and case fatality rate of multi-organ failure are reduced. According to the technical scheme, high prediction precision is kept, meanwhile, balance of interpretability, stability and clinical applicability of the model is achieved, a reliable auxiliary diagnosis tool is provided for organ injury risk assessment of acute poisoning patients, and the method has important clinical application value and wide market prospects.

To provide a method for producing a myokine producing promotor supported by evidence; and to provide various agents exhibiting excellent effects on skeletal muscles and immunization by promoting production of myokine in a body (skeletal muscles, etc.).SOLUTION: There is provided a method of producing a myokine producing promotor, in which, while a temperature of an object to be separated into solid and liquid in a vessel is maintained at 45°C or lower, a pressure inside the vessel is reduced to separate the object into solid and liquid without using both extraction liquid and extraction steam which are extraction media, and the liquid contained in the object is recovered as an active ingredient, the object being an undried product which is cypress not completely dried in the method of producing a myokine producing promotor. There are provided a myokine producing promotor produced by the method, and an anti-inflammatory agent for skeletal muscles, a skeletal muscle formation promoter, a repair agent for damaged skeletal muscles, an enlargement agent for skeletal muscles, and a post-movement immunosuppressant, which contain the myokine producing promotor.SELECTED DRAWING: Figure 4

The application discloses a metformin-induced stem cell-derived extracellular matrix material and a preparation method and application thereof, and relates to the technical field of biological materials. The preparation method comprises the following steps: inoculating mesenchymal stem cells on a pretreated culture carrier, and using a culture system containing metformin to induce and treat the mesenchymal stem cells; adding ascorbic acid to induce and culture extracellular matrix secretion; carrying out decellularization treatment and nuclease treatment on the metformin-induced extracellular matrix; collecting the metformin-induced stem cell-derived extracellular matrix, mixing the metformin-induced stem cell-derived extracellular matrix with an aqueous medium, and enriching and forming an extracellular matrix precipitate through centrifugal treatment; and sequentially carrying out freezing treatment and thawing treatment on the extracellular matrix precipitate to obtain the metformin-induced stem cell-derived extracellular matrix material. The metformin is used to induce the mesenchymal stem cells to secrete an engineered extracellular matrix, and the engineered extracellular matrix is used to promote the repair of bone defects, skeletal muscle injuries and musculoskeletal joint defects in a diabetic environment.

The invention discloses application of plasmalogen in assisting in gaining muscle and reducing fat in aerobic exercise and relieving skeletal muscle and kidney injury caused by exercise, and belongs to the technical field of biological medicine. According to the invention, a model of mouse skeletal muscle and kidney injury caused by exhaustion movement is established in vivo, and skeletal muscle and kidney injury is relieved by intervention of a sea squirt phospholipid extract and sea squirt plasmalogen respectively. Skeletal muscle injury and metabolic waste entering blood of a C57BL / 6J mouse caused by continuous exhaustion exercise for 7 days greatly increase the kidney burden. The sea squirt phospholipid extract and the sea squirt plasmalogen relieve muscle and kidney injury caused by exhaustive exercise and improve exercise performance.

The invention belongs to the technical field of medicines, and particularly relates to application of arginine in repairing skeletal muscle injury caused by monensin poisoning. It is found for the first time that arginine can remarkably recover the normal function of skeletal muscle mitochondria and the normal polymerization capacity of microtubules by up-regulating the expression level of mitochondrial oxidative phosphorylation (OXPHOS) protein complex and regulating microtubule polymerization kinetics and reversing monensin-induced skeletal muscle mitochondrial dysfunction and microtubule network disintegration; the method provides a theoretical basis for clinical transformation, and has good practical application value.

This invention relates to methods for increasing the regeneration of a skeletal muscle that comprise increasing the concentration of Mesencephalic Astrocyte-derived Neurotrophic Factor (MANF) in the skeletal muscle. The concentration of MANF may be increased by administering to the individual MANF, a nucleic acid encoding MANF, or a cell expressing MANF. This may be useful in the treatment of skeletal muscle damage or disease, for example in individuals with an age-related reduction in skeletal muscle regenerative capacity.

The invention discloses an application of fingolimod hydrochloride in preparation of a medicine for promoting skeletal muscle injury repair. According to the prepared medicine, the phenotypic transformation promoting effect, the immunoregulation effect, the myoblast proliferation and differentiation promoting effect and the fibrosis reaction inhibiting effect of fingolimod hydrochloride on macrophages at the skeletal muscle injury part are utilized, so that the local inflammatory response at the skeletal muscle injury part is relieved, myoblast differentiation is promoted, myotube atrophy is reduced, and the skeletal muscle injury part can be cured. Meanwhile, the fibrosis reaction of a skeletal muscle injury part is inhibited, so that remarkable skeletal muscle defect repair is realized. The new application can effectively promote repair and regeneration of muscles at skeletal muscle injury parts, can significantly improve the morphology and quality of the injured muscles, promotes regeneration of new muscle fibers and reduces muscle fibrosis, is suitable for treatment of traumatic or non-traumatic large-volume skeletal muscle defects, and has a wide application prospect. A new theoretical basis can be provided for research and development of skeletal muscle defect repairing injections, and the skeletal muscle defect repairing injections have great scientific significance and clinical transformation value.

The invention belongs to the technical field of medicines, and particularly relates to application of arginine in repairing skeletal muscle injury caused by monensin poisoning. It is found for the first time that arginine can remarkably recover the normal function of skeletal muscle mitochondria and the normal polymerization capacity of microtubules by up-regulating the expression level of mitochondrial oxidative phosphorylation (OXPHOS) protein complex and regulating microtubule polymerization kinetics and reversing monensin-induced skeletal muscle mitochondrial dysfunction and microtubule network disintegration; the method provides a theoretical basis for clinical transformation, and has good practical application value.

This invention discloses a multifunctional injectable hyaluronic acid hydrogel, its preparation method, and its applications. The hydrogel comprises: a methacrylamide hyaluronic acid hydrogel matrix; cobalt-containing composite microspheres dispersed in the matrix, wherein the cobalt-containing composite microspheres are gelatin methacrylate / alginate methacrylate microspheres loaded with citric acid-coated cobalt nanoparticles; and miR-206-3p-enriched exosomes loaded in the hydrogel. The multifunctional injectable hyaluronic acid hydrogel provided by this invention organically integrates the hyaluronic acid matrix, cobalt-containing functional microspheres, and miR-206-3p-enriched exosomes, and can simultaneously provide multiple functions such as structural support, local gelation, angiogenesis promotion, and myogenic differentiation promotion. It overcomes the shortcomings of single materials in meeting the comprehensive repair needs of acute skeletal muscle injury, and provides a comprehensive repair strategy for acute skeletal muscle injury that combines structural support, angiogenesis induction, myogenic differentiation regulation, and anti-fibrosis functions.

A skeletal muscle injury experiment device relates to the technical field of medical experiment equipment and comprises an experiment table, a bracket, a sleeve, a sliding rod, a balancing weight, an experiment piece and a locking piece, the support is fixedly connected to the experiment table, and the sleeve is connected to the support. The two ends of the sliding rod are detachably connected with the balancing weight and the experiment piece. The sleeve is provided with a sleeve cavity; the sliding rod is slidably connected with the sleeve, and at least part of the sliding rod is located in the sleeve cavity; the balancing weight is located outside the cavity of the sleeve. The locking piece is connected with the sleeve and the sliding rod, the locking piece can enable the sliding rod to be locked at the preset height of the sleeve, and the locking piece is configured to be capable of unlocking the sliding rod when being opened, so that the sliding rod moves along the cavity of the sleeve under the action of gravity and drives the experiment piece to downwards abut against or approach the experiment table. The utility model provides a skeletal muscle injury experimental device, which aims to solve the technical problems of complicated operation, poor experimental repeatability and poor stability of experimental results in the prior art.

The invention relates to the technical field of biological medicine, in particular to application of lncRNA as a target spot in tissue damage repair. Experimental exploration finds that lncRNA Brip1os participates in repair after tissue injury, particularly participates in early repair after liver and skeletal muscle injury, and possibly regulates regeneration of skeletal muscle through an interferon (IFN) related mechanism.

The invention discloses a composition for promoting skeletal muscle injury regeneration and application thereof, and belongs to the technical field of medicines. In order to solve the technical problems that in the prior art, the skeletal muscle injury regeneration promotion effect of nutrients is insufficient, and the new application of eleutheroside B in the field is undefined, the invention provides a composition for promoting skeletal muscle injury regeneration, and the composition effectively shortens the regeneration time of skeletal muscle after chemical injury or mechanical injury and improves the skeletal muscle regeneration effect. The maturity of new muscle fibers is improved; the composition can be in oral dosage forms such as instant powder, oral liquid or tablets, can take effect on the day of injury, does not need local injection, is convenient to use, and is suitable for preparing functional food or formula food for special medical purposes for shortening the regeneration time of skeletal muscle after injury and improving the maturity of new muscle fibers. And a new technical scheme is provided for nutrition intervention of skeletal muscle injury.

The invention discloses a skeletal muscle injury time inference method based on an ATR-FTIR spectrum, and relates to the technical field of biomedicine. The method specifically comprises the following steps: material preparation and primary treatment: dividing experimental animals into a plurality of injury time groups at different intervals after death, applying closed contusion, and collecting tissue samples at different time points; carrying out ATR-FTIR spectrum and spectrum pretreatment; the PCA algorithm is used for evaluating the influence of the death interval and injury on skeletal muscles, it is proved that the death interval obviously deduces the injury time, then attempts of avoiding changes after death and establishing an injury time deduction regression model are performed based on the PLS algorithm, and the feasibility of the scheme is proved. According to the method, the ATR-FTIR spectrum is combined with multiple machine learning algorithms, so that the injury time of the skeletal muscle can be more accurately deduced; a regression model conforming to damage time sequence change is constructed instead of a traditional classification model; and continuous changes in the injury process can be captured.

The invention relates to the field of application of tea leaves, in particular to application of catechin in preparation of a reagent for repairing bone injury caused by obesity. In order to solve the problem that in existing research, the action mechanism of main catechin monomers for repairing the bone injury caused by obesity is not clear, four catechin monomers EC, ECG, EGC and EGCG are screened and applied to repairing the bone injury caused by obesity, and it is found through determination of related indexes of mouse skeletal muscles that the function mechanism of the main catechin monomers for repairing the bone injury caused by obesity is not clear. ECG has the strongest ability of repairing skeletal muscle injury caused by obesity, and the ECG regulates skeletal muscle injury by means of inflammation expression of obesity, skeletal muscle lesion, muscle homeostasis and the like; by adjusting fatty acid, blood fat is reduced, and adipogenesis is inhibited; glutamic acid is adjusted, muscle strength, endurance and quality are increased, and a protection effect on bone damage is achieved. The method is an important supplement in the field of scientific research of tea leaves, and provides a scientific basis for application of functional components of the tea leaves.

The invention discloses a lactobacillus plantarum WY2401 for promoting muscle growth and development and application of the lactobacillus plantarum WY2401. The lactobacillus plantarum is used for preparing medicines for treating and / or preventing skeletal muscle injury, promoting muscle regeneration or improving muscle functions, the lactobacillus plantarum WY2401 is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO.M2025499. The lactobacillus plantarum WY2401 can be used for preparing medicines for treating and / or preventing skeletal muscle injury, promoting muscle regeneration or improving muscle functions. The invention relates to application of lactobacillus plantarum WY2401 in preparation of functional food, health care products or probiotic supplements for promoting muscle regeneration or improving muscle functions, application of lactobacillus plantarum in preparation of a model for improving muscle development and / or promoting muscle regeneration, and application of the model in screening of drugs and health care products. Or clinical diagnosis and evaluation of a treatment scheme. The invention discloses a new action mechanism of the lactobacillus plantarum WY2401, so that application is developed.

The invention discloses application of miR-126 and an exosome carrying the miR-126 in promotion of skeletal muscle injury repair and proliferation and differentiation, and belongs to the field of regenerative medicine. The invention provides application of miR-126 with a nucleotide sequence as shown in SEQ ID NO.1 or an exosome carrying the miR-126 in preparation of drugs for promoting skeletal muscle injury repair and products for promoting skeletal muscle proliferation and differentiation. Experimental results show that overexpression miR-126 mature body or miR-126-carrying exosome treatment promotes proliferation and differentiation of muscle cells in an in-vitro experiment; in an in-vivo experiment, muscle tissue repair of a mouse with skeletal muscle injury is promoted; in short, miR-126 is a positive regulatory factor for skeletal muscle development and regeneration. The invention provides a new strategy for the improvement of meat yield and meat quality of meat animals and the treatment of skeletal muscle diseases, and has great economic significance and wide application prospects.

The application discloses a device for positioning and quantifying a tibialis anterior muscle injury model of rodents, and belongs to the technical field of medical equipment, which comprises a shearing assembly and a positioning and measuring device, the positioning and measuring device is arranged on the shearing assembly, the positioning and measuring device comprises a measuring assembly for defining the size of a skeletal muscle injury and a positioning assembly for positioning a tibialis anterior muscle part, the measuring assembly is arranged on the positioning assembly, the measuring direction of the measuring assembly is perpendicular to the positioning direction of the positioning assembly, the positioning direction of the positioning assembly is perpendicular to the shearing direction of the shearing assembly, and the lengths of the two ends of the positioning assembly are equal to the length of the output end of the shearing assembly; the device can help researchers to position and quantify the establishment of a tibialis anterior muscle injury model of rodents.

A method of treating injured skeletal muscle tissue generally includes applying to injured skeletal muscle tissue a therapeutic composition that includes a purified exosome product (PEP) and a pharmaceutically acceptable carrier. In one or more embodiments, the method includes applying from 1 x 1011 PEP exosomes to 1 x 1013 PEP exosomes to the injured skeletal muscle. In one or more embodiments, the therapeutic composition further includes a supportive matrix such as a collagen scaffold, a tissue sealant, fibrin glue, or a hydrogel.