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Method for controlling carbon supplement culturing of microalgae through pH value feedback

A feedback control and microalgae technology, applied in biochemical equipment and methods, tissue cell/virus culture devices, single-cell algae, etc., can solve problems such as interference with pH control, poor carbon supplementation, and difficult control. Achieve the effects of eliminating mutual interference, shortening the production cycle, and facilitating implementation

Inactive Publication Date: 2006-01-25
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0007] An object of the present invention is to overcome the use of NaHCO 3 The effect of carbon supplementation is not good, and it will increase the pH of the culture system and increase the cost; while direct introduction of CO 2 Difficult to control CO when replenishing carbon 2 The amount of addition just meets the consumption of algae cells, and at the same time interferes with the control of pH itself, etc., providing a method for cultivating microalgae by adding carbon dioxide through pH feedback control

Method used

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  • Method for controlling carbon supplement culturing of microalgae through pH value feedback

Examples

Experimental program
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Effect test

Embodiment 1

[0031] See figure 1 , wherein the pH sensor 1 is a commercially available steam sterilizable pH electrode, the control device 2 is a pH meter with switch control, CO 2 The gas source device 3 is provided by a steel cylinder, the actuator 4 is a two-position normally closed solenoid valve (diameter 8 mm), and the microalgae cultivation device 5 is a 20-liter air-lift photobioreactor (Chinese patent ZL99213569.9). The supply device 6 is an air compressor (supplies air). Necessary flow meters, filters and valves are also installed in the pipeline. Haematococcus pluvialis (Haematococcus pluvialis-F712, freshwater algae species bank of the Type Culture Collection Committee of the Chinese Academy of Sciences) was cultivated in the 20-liter airlift photobioreactor.

[0032] The medium was SM medium prepared with ordinary tap water.

[0033] The medium in the airlift photobioreactor was boiled for 20 minutes to sterilize by introducing hot steam, and after cooling, the seed solutio...

Embodiment 2

[0036] The equipment used is the same as in Example 1. Dunaliella salina (Dunaliella salina 1009, Institute of Salt Manufacturing, Ministry of Light Industry, Tanggu, Tianjin) was cultivated in a reactor.

[0037] The medium is ASP prepared in distilled water 2 Culture medium (remove the vitamin solution and buffer). In order to avoid precipitation when preparing the medium, ASP 2 MgSO in medium 4 and CaCl 2 Dissolve separately first, then add the two ingredients after the other ingredients have dissolved and diluted with sufficient distilled water. NaCl was then added to a final concentration of 12%.

[0038] The culture medium is not sterilized, and the seed solution is directly inserted through the inoculation port of the reactor, and the volume of the culture medium in the reactor is 16 liters. The initial algae cell density was 8×10 4 cells / ml. The temperature in the reactor was controlled at 30±0.5°C. Illumination is provided by 15 36W fluorescent lamps on the p...

Embodiment 3

[0041] The equipment used is the same as in Example 1. Spirulina platensis (Spirulina Platensis, 439, Freshwater Algae Species Bank of Type Culture Collection Committee, Chinese Academy of Sciences) was cultivated in the reactor.

[0042] The medium was Zarrouk's medium prepared with deionized water.

[0043] Feed hot steam to make the culture medium boil for 20 minutes to sterilize, and after cooling, insert the seed solution through the inoculation port of the reactor, and the volume of the culture medium in the reactor is 16 liters. The initial algal cell concentration was 0.02g / L. The temperature in the reactor was controlled at 30±1°C. The light intensity is 127 μmol photon m -2 the s -1 . On the control instrument 2, the pH value of the culture solution was set at 9.4, the flow rate of air was 5.0 L / min, and the flow rate of carbon dioxide was 70 mL / min (the solenoid valve was open) or 0 (the solenoid valve was closed). During the cultivation process, the instrume...

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Abstract

The invention relates to a method that could cultivate algae by PH feedback control to add CO2 that belongs to tiny algae cultivating field. The PH value of culture solution is set in a certain range, and according to the difference between the practical test vale of the PH value in the culture solution and the preset value, the CO2 would be added to make the PH value be in the certain range of the preset value. It would not be finished until the condition that could stop cultivating emerges. The invention realizes automatically adding carbon and automatically controlling PH value for algae cultivation.

Description

technical field [0001] The invention belongs to the field of microalgae cultivation, in particular to a method for cultivating microalgae by adding carbon dioxide through pH feedback control. technical background [0002] Microalgae are rich in protein, polysaccharides, unsaturated fatty acids and other nutrients (such as spirulina), which can be used in food and medicine; contain a variety of pigments (such as Porphyridium), which can be used in cosmetics, bioengineering and medical diagnosis; A large amount of hydrocarbons are accumulated, and the hydrocarbon content can account for more than 50% of the dry weight (such as Botrytis), which can be directly used for power generation; some microalgae can fix nitrogen and can be used as natural fertilizers (such as Anabaena), controlled by nutrition It can be used as high-quality bait; some microalgae also contain antibiotics and toxins, which are of great medicinal value. Microalgae have become raw materials for food, health...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12M3/00
Inventor 丛威康瑞娟蔡昭铃
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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