Method for producing biodiesel using high-cell-density cultivation of microalga Chlorella protothecoides in bioreactor

a bioreactor and microalga chlorella technology, applied in biofuels, biofeedstock, biofuels, etc., can solve the problems of high cost of biodiesel, inability of conventional oil/food crop-based biodiesel production system to meet the growing demand for sustainable feedstock for biodiesel production, and no biodiesel production technology by microalgae is economically feasible, etc., to achieve the effect of reducing acidity

Inactive Publication Date: 2009-08-27
TSINGHUA UNIV
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Benefits of technology

[0022]Yet another preferred embodiment of the present invention further comprises adding a base to reduce acidit

Problems solved by technology

The high cost of biodiesel, which is mainly due to the high cost of oil feedstock, is the main obstacle to its broader commercialization.
Due to their low oil yield potential and high demands on land, water, and fertilizer, which create competition with food/feed industries, the conventional oil/food crop-based biodiesel production system cannot meet the growing demand on sustain

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  • Method for producing biodiesel using high-cell-density cultivation of microalga Chlorella protothecoides in bioreactor
  • Method for producing biodiesel using high-cell-density cultivation of microalga Chlorella protothecoides in bioreactor
  • Method for producing biodiesel using high-cell-density cultivation of microalga Chlorella protothecoides in bioreactor

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Materials and Methods

[0106]The original strain of C. protothecoides came from the Culture Collection of Alga at the University of Texas (Austin, Tex.), which has been kept in Department of Biological Science and Biotechnology in Tsinghua University since 1990. The compositions of the culture medium for this strain are:[0107]KH2PO4: 0.7 g / L,[0108]K2HPO4: 0.3 g / L,[0109]MgSO4.7H2O: 0.3 g / L,[0110]FeSO4.7H2O: 3 mg / L,[0111]Glycine: 0.1 g / L,[0112]vitamin B1: 0.01 mg / L,[0113]A5 trace mineral solution 1.0 ml / L.

[0114]By oriented selection and screening as described in step (1) above, the specific strain with characteristics of high cell growth rate and high oil content is obtained as Chlorella. Protothecoides sp 0710. Comparing with the original cells, the Chlorella. Protothecoides sp 0710 is in golden color, cell dry weight approximately 108 g / l with oil / lip content at about 61% of cell dry weight. Table 1 illustrates compositions of biodiesel produced by the Chlorella. Protothecoides sp 071...

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Abstract

A method is provided to produce biodiesel from algae using a strain of microalga chlorella protothecoids, by screening a specific strain with characteristics of high yield of biomass and high oil content, cultivating the screened strain for high-cell-density growth for up to 108 grams of dry cell weight per liter of the suspension in a bioreactor using solutions containing carbohydrates as feed, harvesting and drying the high density cultivated algal cells to extract oil from the dried algal cells, and producing the biodiesel by reaction of catalyzed transesterification using the extracted oil as feedstock.

Description

CROSS REFERENCE TO RELATED APPLICATION[0001]This application claims the benefit of China Patent Application No. 200810100871.5, filed on Feb. 25, 2008, and entitled “Method for Producing Biodiesel using High-Cell-Density Cultivation Of Microalga Chlorella Protothecoides”, the entire disclosure of which is incorporated herein by reference.FIELD OF THE INVENTION[0002]The present invention relates to area of regenerable biofuel, in particular to a method for producing biodiesel from microalga oil by high-cell-density heterotrophic cultivation of a specific strain of green alga Chlorella protothecoides rich in lipids coupled with biodiesel production by transeterification reaction.BACKGROUND OF THE INVENTION[0003]Renewable carbon-neutral fuels, such as biodiesel, are considered necessary replacement of traditional petroleum fuel for environmental and economic sustainability. Biodiesel is one such fuel composed of monoalkyl esters of long chain fatty acids transesterified from vegetable ...

Claims

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Application Information

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IPC IPC(8): C10L1/18
CPCC11B1/10C11C3/003Y02E50/13C12P7/649C12N1/12Y02E50/10Y02P30/20
Inventor WU, QINGYUZHOU, WENGUANGXIONG, WEI
Owner TSINGHUA UNIV
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