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A method for industrial production of biocatalysts in the form of enzymes or microorganisms immobilized in polyvinyl alcohol gel, their use and devices for their production

A polyvinyl alcohol gel and biocatalyst technology, which is applied in the methods of granulating raw materials, chemical instruments and methods, biofuels, etc., can solve the problems of product shape change, mechanical wear of continuous conveyor belts, etc., and reduce the reaction volume. , the effect of reducing operating costs and high reaction rates

Active Publication Date: 2009-05-06
澳源泰科环保科技(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Another disadvantage is the significant mechanical wear of the continuous conveyor belt of the casting unit
It is further understood that the use of a salt solution in the re-expansion section of the casting unit causes the salt to adhere to the conveyor belt surface and thus causes a change in the surface tension of the continuous conveyor belt of the casting unit, which in turn leads to a change in the shape of the product

Method used

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  • A method for industrial production of biocatalysts in the form of enzymes or microorganisms immobilized in polyvinyl alcohol gel, their use and devices for their production
  • A method for industrial production of biocatalysts in the form of enzymes or microorganisms immobilized in polyvinyl alcohol gel, their use and devices for their production
  • A method for industrial production of biocatalysts in the form of enzymes or microorganisms immobilized in polyvinyl alcohol gel, their use and devices for their production

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Immobilization – biocatalyst for nitrification by embedding pure mixed cultures of nitrifying bacteria (Nitrosomonas europeae and Nitrobacter winogradskiy) in PVA-hydrogel prepared.

[0072] Immobilization - The biocatalyst for denitrification was prepared by entrapment of paraccocus denitrificans.

[0073] The immobilization was carried out according to the following method: a PVA solution containing 100 g of PVA (polyvinyl alcohol), 60 g of PEG (polyethylene glycol), and 790 g of distilled water was prepared. Add 50 ml of homogenized biomass suspension to the prepared solution such that the resulting cell concentration reaches 0.60 g cells / dm 3 gel. The cell mixture in the PVA solution thus prepared was dropped on a rigid plate, then cross-linked and shaped in a stream of dry air at 80°C to 15°C. Make the surface area to volume ratio not less than 7mm -1 The immobilized substance was transferred to a solution of sodium sulfate (0.01 mol / l) and ammonium chloride (0...

Embodiment 2

[0079] Immobilized substances prepared and cultured as described in Example 1 above were used in a continuously regulated mode of nitrification and denitrification to remove nitrogen pollution from real wastewater containing high concentrations of nitrogen pollution (200 mg / l to 1000 mg / l N- NH 4 ) and actually has a dichromate value of 0.

[0080] A continuous mode unit was used to detect simultaneous nitrification and denitrification, with an effective volume of 17 L for the oscillatory nitrification reactor and 3.8 L for the oscillatory denitrification reactor. The flow rate of actual industrial wastewater is 35 liters / day, where N-NH 4 The content is 1600mg / l.

[0081] 2000 g of biocatalyst containing embedded nitrifying bacteria was added to the nitrification reactor. 800 g of biocatalyst containing embedded denitrifying bacteria was added to the denitrification reactor.

[0082] In the case of 95% nitrification efficiency, the average nitrification rate is about 1000...

Embodiment 3

[0084] Immobilized substances prepared and cultured as described in Example 1 above were used in a regulated mode of continuous nitrification and denitrification to remove nitrogen pollution from real wastewater containing high concentrations of nitrogen pollution (200 mg / l to 1000 mg / l N -NH 4 ) and high dichromate values ​​(>1000mg / l).

[0085] The prepared immobilized products were subjected to batch fermentation. In a working volume of 17 liters containing 15 liters of synthetic wastewater (WW) containing approximately 800 mg / l N-NH 4 and 2000g of biocatalyst containing embedded nitrifying bacteria), the nitrification process (removal of nitrogen from ammonia water) was completed after 12 hours, which means that the nitrification rate was about 500mg N-NH 4 / hour x kg biocatalyst, equivalent to about 1.6kg N-NH respectively 4 / m 3 WW x days and 12kg N-NH 4 / m 3 Biocatalyst x number of days.

[0086] In an effective volume of 15.8 liters, containing 15 liters of synt...

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Abstract

A method for the industrial production of the biocatalysts with biologically active material in the form of immobilized enzymes or microorganisms which are immobilized into the polyvinyl alcohol gel, and their use based on the fact, that the active biological material, formed by the mixture of free native or pretrated (aggregated) enzyme catalyst, or production microorganism, or part of them, and the polyvinyl alcohol gel, is used for their industrial production, the mixture is gelated and shaped in a stream of drying air at the temperature of 800C to 150C, considering the extent of the biologically active material, at the biocatalyst geometrical ratio of the surface to the volume kept larger than 7 mm'1, and consequently thus prepared biocatalysts can be cultivated or stored and then use in biotechnological processes in the conditions,; which ensure given biotechnological process higher productivity, higher production and enzymatic stability, long-term and repeated usage or definable process control with consequence easy separation of the biocatalyst. The industrial production device, providing optimization of the biological carrier volume and surface in dependence on biologically active material extent, consisting of a casting mechanism (17) mounted in front of a drying channel (2), through which a continuous conveyor belt (1) runs, is equipped with at least one casting head (17) with two rows of casting needle injectors connected to a pressure tempered tank (15) and a compressor (16), the conveyor belt (1) and a drying system - the source (4) of a drying air, which is blown by means of ventilator into an air distribution system (6) with incorporated heating elements (5),; which runs into the upper drying channel (2), and further the lower final drying channel (3) and a reswelling tank (7), between which a wiping and collecting device (9) is mounted, designed on the basis of mechanical wiping and high-pressure rinse.

Description

field of invention [0001] The present invention relates to a process for the industrial production of biocatalysts with biologically active materials in the form of immobilized enzymes or microorganisms immobilized in polyvinyl alcohol gels, as well as the use of said biocatalysts and an industrial production device, which The volume and area of ​​the biocarrier can be optimized according to the range of bioactive materials, which includes a casting device placed in front of a drying tunnel through which a continuous conveyor belt runs. Background of the invention [0002] The use of polyvinyl alcohol carriers or polyurethane carriers to embed bacteria is known in the art. CZ patent 249 179 discloses a method for the production of biocatalysts using bioactive materials in the form of microorganisms, enzymes, spores and / or cells placed in a polyvinyl alcohol gel (hereinafter denoted PVA gel). PVA gel is very suitable as a gel carrier for bioactive materials in the production...

Claims

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Application Information

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IPC IPC(8): A61K9/00F26B17/02B01J2/00
CPCY02E50/17
Inventor R·斯鲁卡尔M·罗森伯格M·瑞布罗斯
Owner 澳源泰科环保科技(北京)有限公司
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