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Peach gum hydrolase producing strain and application in preparation of peach gum polysaccharide thereof

A technology for producing strains and decomposing enzymes, which is applied in the directions of microorganism-based methods, microbial determination/inspection, biochemical equipment and methods, etc., which can solve the problem of peach gum product properties and production research reports are few, and peach gum decomposition has not yet been seen. Using enzymes and other issues to achieve the effects of being conducive to sustainable development, promoting comprehensive utilization and development, and narrowing the relative molecular mass distribution

Inactive Publication Date: 2010-05-05
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are not many research reports on the properties and production of peach gum products
However, there are no relevant reports on the production of peach gum polysaccharides by using microbial fermentation to produce peach gum decomposition enzymes.

Method used

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  • Peach gum hydrolase producing strain and application in preparation of peach gum polysaccharide thereof
  • Peach gum hydrolase producing strain and application in preparation of peach gum polysaccharide thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] The screening of embodiment 1 bacterial strain obtains

[0052] In this example, the deteriorated carrageenan is used as the raw material, and the bacterial strains capable of degrading carrageenan are screened first, and then the bacterial strains capable of degrading the original peach gum are selected through two screening conditions: carrageenan degradation ability and original peach gum degradation ability. , and then combined with bacterial strains to produce enzymes to domesticate, so as to finally obtain a bacterial strain that can efficiently degrade peach gum. The specific steps are as follows:

[0053] 1. Screening of strains capable of decomposing carrageenan

[0054] (1) Microbiological separation of metamorphic carrageenan was carried out by the dilution plate method, and the sample dilution was 10 -2 、10 -3 、10 -4 and 10 -5 , respectively coated on the nutrient agar medium plate and the wort medium plate, 3 plates for each concentration, colony isolat...

Embodiment 2

[0069] The identification analysis of embodiment 2 bacterial strains

[0070] In this example, the strain obtained by screening in Example 1 was identified to identify the strain with strong peach gum decomposing ability.

[0071] 1.16SrDNA sequencing

[0072] Extract DNA from the strain, use a nucleic acid / protein analyzer to measure under the Nucleic Acid program, detect DNA purity and concentration, and perform PCR amplification. The blank control contains all components except the template nucleic acid.

[0073] The amplified product was sent to a sequencing company for sequencing, and the obtained base sequence was searched for homologous sequences (blast search) in international nucleic acid sequence databases such as GenBank through the Internet, and it was found that the strain was 99% homologous to Microbacterium % or more, its morphological characteristics and physiological and biochemical characteristics are most consistent with Microbacterium.

[0074] 2. Physiol...

Embodiment 3

[0078] Example 3 bacterial strain produces the character identification of peach gum decomposing enzyme

[0079] 1. Determination of Peach Gum Polysaccharides

[0080] 1) Drawing of standard curve

[0081] Preparation of glucose standard solution: Accurately weigh 100mg of glucose, first dissolve it with a small amount of distilled water, then transfer to a 100ml volumetric flask to obtain a standard glucose solution of 1.00mg / ml. Accurately take 0.00, 0.50, 1.00, 1.50, 2.00, 2.50ml of 1.00mg / ml glucose standard solution and add them to 50ml volumetric flasks respectively, add 5ml of DNS each, cook in boiling water bath for 5min, cool down rapidly in running water, shake at constant volume Evenly, blank to zero. Optical density values ​​were determined at an absorption spectral wavelength of 540 nm.

[0082] 2) Sample determination

[0083] Take 1ml of each stock solution in a 50ml volumetric flask, and other steps are the same as that of the standard curve, measure the op...

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Abstract

The invention discloses a peach gum hydrolase producing strain and an application in preparation of peach gum polysaccharide thereof. The peach gum hydrolase producing strain belongs to microbacterium, is named microbacterium A5. The microbacterium was conserved in the China Center for Type Culture Collection on august 7, 2009 and the CCTCC NO is M209174. The strain of the invention can be used to effectively produce peach gum hydrolase used for degrading peach gum so as to obtain peach gum polysaccharide with large economic value. The strain of the invention can be utilized to produce peach gum through enzyme method, wherein the hydrolysis period is short, the relative molecular mass distribution of the product is narrow, no environmental pollution is caused, and the method is easy to control. The invention provides a new gum biotechnological process with more environmental friend and uniform product quality so as to facilitate the sustainable development of the society, promote the comprehensive utilization of wastes of Chinese agricultural culture and have wide market prospect.

Description

technical field [0001] The invention relates to the field of preparation of peach gum polysaccharides, in particular to a peach gum decomposing enzyme production strain and its application in preparing peach gum polysaccharides. Background technique [0002] The research, development and production of gum arabic substitutes are very urgent and necessary. New products that replace gum arabic can effectively control the stable market supply. Peach gum is the only natural vegetable adhesive that can replace gum arabic. [0003] The gum secreted by the trunks of peaches, plums, apricots, cherries, etc. is called peach gum. Peach gum is a translucent polysaccharide substance with a wide range of uses. It can be used in light and chemical fields such as food, medicine, cosmetics, printing, and fiber. In the printing industry, it is used as a gold powder adhesive, etc. The industrial production of peach gum in our country is still in its infancy. There are some manufacturers pro...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12Q1/04C12P19/14C12R1/01
Inventor 冉艳红杨曼娜陆俏颖戚彩娃
Owner JINAN UNIVERSITY
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