Amycolatopsis sp. YIM M13163 and method for preparing efrotomycin pivalate by adopting same

A technology of amycobacterial acid bacteria, eromycin pivalate, applied in the directions of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problem of lack of eromycin, hindering research, and scarcity of source routes and other problems, to achieve the effect of high product yield and simple and easy implementation.

Active Publication Date: 2016-08-24
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the scarcity of sources, this compound was only discovered in a small amount in

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Amycobacterium pseudobacterium ( Amycolatopsis sp. ） YIM M13163 isolation and identification

[0037] 1. Strain isolation

[0038] (1) Amycobacterium pseudobacterium ( Amycolatopsis sp.) YIM M13163 was isolated from seabed sediments in the South my country Sea.

[0039] (2) The mature aerial spores of the above-mentioned isolated strains are white, and the hyphae in the substrate are orange-red.

[0040] 2. Strain identification

[0041] Genomic DNA of the above isolated strain was extracted, its 16S rRNA was amplified by PCR by conventional methods, and sequenced and analyzed, it was shown that it was different from the currently published strains of Amycobacterium pseudobacterium.

[0042] At the same time, combined with other identification data results, the strain is Amycolatopsis A new strain of the genus, which was named Amycolatopsis sp. YIM M13163 was deposited in the China Center for Type Culture Collection on July 10, 2015, with...

Embodiment 2

[0043] Example 2 Preparation and identification of emomycin pivalate

[0044] 1. Fermentation of Amycobacterium pseudobacterium YIM M13163

[0045] (1) The formulations of seed medium and fermentation medium are: soluble starch 2g / L, glucose 20g / L, yeast extract powder 2g / L, peptone 2g / L, K 2 HPO 4 0.5g / L, MgSO 4 ·7H 2 O 0.5g / L, CaCO 3 2g / L, 25g / L sea salt, NaCl 4g / L.

[0046] (2) Cultivation of seeds:

[0047] The activated amycobacteria ( Amycolatopsis sp.) YIM M13163 was inserted into a 250ml Erlenmeyer flask containing 50ml of seed medium, and cultured on a shaker at 28°C and 200rpm for 36 hours to obtain a seed solution;

[0048] In a 1L Erlenmeyer flask equipped with 200ml of fermentation medium, culture at 28°C and 200rpm for 9 days to obtain a fermentation culture.

[0049] (3) Large-scale fermentation culture:

[0050] Transfer 50 mL of the seed culture solution in the above Erlenmeyer flask to a 1L Erlenmeyer flask containing 200 mL of fermentation m...

Embodiment 3

[0122] Example 3 Antibacterial Activity of Elothromycin Pivalate

[0123] The bacteriostasis activity of elothromycin pivalate among the present invention is tested, and the result shows that it has certain bacteriostasis activity to Pseudomonas aeruginosa, Escherichia coli, and has stronger bacteriostasis activity to Bacillus thuringiensis .

[0124] In addition, when performing MIC (Minimum Inhibitory Concentration) detection, the measured MIC value of emomycin pivalate against Bacillus thuringiensis reached 2 μg / mL.

[0125] These good biological activities indicate that emoxicin pivalate has potential drug development value. Moreover, the method for preparing elothromycin pivalate in the present invention is simple and has high yield, which is of great significance for the development, application and popularization of elothycin.

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Abstract

The invention discloses Amycolatopsis sp. YIM M13163 and a method for preparing efrotomycin pivalate by adopting the strain through fermentation. Amycolatopsis sp. YIM M13163 is preserved in the China Center for Type Culture Collection (CCTCC) on July 10, 2015, the preservation number is CCTCC M 2015445, and the preservation address is Wuhan University in Wuhan city of China. The Amycolatopsis sp. YIM M13163 provided by the invention can generate antibiotic, namely, efrotomycin pivalate through fermentation; meanwhile, the method for preparing efrotomycin pivalate by adopting the strain is simple, the yield is high, thus sufficient sample support is provided for the development of the medicinal value of efrotomycin pivalate, and therefore, the application prospects are wide.

Description

technical field [0001] The invention belongs to the technical field of industrial microorganisms, and in particular relates to a marine actinomycete Amycobacterium pseudobacterium ( Amycolatopsis sp.) YIM M13163 and a method for preparing erotomycin pivalate using the strain. Background technique [0002] Erotomycin (efrotomycin) has a wide range of biological activities, such as in vitro experiments, it is Moraxella, Pasteurella, Yersinia pestis, Haemophilus, Streptococcus and rod Bacillus and other animal pathogenic bacteria have good antibacterial activity. The antibacterial activity test of elothromycin showed that it had slightly weak antibacterial activity against Pseudomonas aeruginosa and Escherichia coli, but had strong antibacterial activity against Bacillus thuringiensis. [0003] These good biological activities indicate that elothmycin has potential drug development value. However, due to the scarcity of sources, this compound was only discovered in a small ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P19/26C12R1/01
Inventor 李文均刘晴陈微高锐段焰青侯伟鞠建华
Owner SUN YAT SEN UNIV
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