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A method for preparing echinosporin antibiotics by fermentation

A technology of antibiotics and mycocins, applied in the field of industrial microorganisms, can solve the problems of undiscovered public reports

Inactive Publication Date: 2021-09-07
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The echinosporin antibiotics in the prior art are all fermented by Streptomyces, and through literature search, no public reports of the same documents as the content of the present invention are found. The present invention provides a new method for the production and preparation of echinosporin antibiotics, new way

Method used

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  • A method for preparing echinosporin antibiotics by fermentation
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The preparation method of compound (1) and (2)

[0028] (1) strain liquid seed culture: 1. the preparation of liquid seed culture medium: get yeast extract 4.0g; Glucose 4.0g; Malt extract 5.0g; Multivitamin 3.5mg; Add water to 1000ml, pH 7.2, 120 ℃ of sterilization 30min ; ② Fermentation: inoculate the bacterial strain YIM PH20520 into the sterilized medium, and ferment for 3 days on a shaking table with a rotating speed of 220 rpm at 28°C.

[0029] (2) Strain fermentation culture: ① Preparation of fermentation medium: get 24.0g of starch; 3.0g of beef extract; 1.0g of glucose; 5.0g of yeast extract; 3.0g of peptone; 34.0g; add water to 1000ml, pH 7.0, sterilize at 120°C for 30 min; 2. ferment: add the bacterial strain YIM PH20520 seed liquid obtained in the above method (1) to the sterilized fermentation medium, add the amount of bacteria It is 10% of the weight of the fermentation medium, and the flask is fermented for 6 days at 28 ° C on a shaker with a rotation sp...

Embodiment 2

[0033] The cytotoxic activity of the compounds (1) and (2) prepared in Example 1 on tumor cells was determined by conventional MTT method.

[0034] Experiments showed that compounds (1) and (2) inhibited the IC of human colon cancer cell line HCT116 50 The values ​​(µg / mL) were 2.23 and 8.56 respectively, and the IC of inhibiting human cervical cancer Hela cell line 50 The values ​​(µg / mL) were 3.23 and 23.18 respectively, and the IC of inhibiting human gastric adenocarcinoma cell line BGC-823 50 The values ​​(µg / mL) were 3.23 and 45.68 respectively, and the IC of inhibiting human liver cancer cell line HepG-2 50 The values ​​(µg / mL) were 3.43 and 46.68 respectively.

Embodiment 3

[0036] The preparation method of compound (1) and (2)

[0037] (1) strain liquid seed culture: 1. the preparation of liquid seed culture medium: get yeast extract 4.0g; Glucose 4.0g; Malt extract 5.0g; Multivitamin 3.5mg; Add water to 1000ml, pH 7.2, 120 ℃ of sterilization 30min ; ② Fermentation: inoculate the bacterial strain YIM PH20520 into the sterilized medium, and ferment for 3 days on a shaking table with a rotating speed of 220 rpm at 28°C.

[0038] (2) Strain fermentation culture: ① Preparation of fermentation medium: get 20.0g of starch; 1.0g of beef extract; 0.5g of glucose; 3.0g of yeast extract; 1.0g of peptone; 3 2.0g; add water to 1000ml, pH 7.0, sterilize at 120°C for 30 min; 2. ferment: add the bacterial strain YIM PH20520 seed liquid obtained in the above method (1) to the sterilized fermentation medium, add the amount of bacteria It is 10% of the weight of the fermentation medium, and the flask is fermented for 5 days at 28 ° C on a shaker with a rotation ...

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Abstract

The invention relates to a method for preparing echinosporin antibiotics by fermentation of amycobacteria, which is characterized in that a strain of amycobacteria with a registration number of CCTCC NO: M 2017189 is cultured and preserved by fermentation ( Amycolatopsis speibonae ) YIM PH20520, and then the fermentation product was extracted, concentrated, separated and purified to obtain a molecular weight of 223 and a molecular formula of C 10 h 9 NO 5 echinosporin and its molecular weight is 207, its molecular formula is C 10 h 9 NO 4 7-deoxyechinosporin, the present invention provides a new method and a new approach for the production and preparation of echinosporin antibiotics.

Description

technical field [0001] The invention relates to a method for preparing echinosporin antibiotics by fermentation of amycobacteria pseudobacterium, which belongs to the field of industrial microorganisms. Background technique [0002] Echinosporin antibiotics include the compounds Echinosporin (Echinosporin) and 7-Deoxyechinosporin (7-Deoxyechinosporin), and their structures are shown in formula (I). Streptomyces strains Streptomyces echinosporus MK-213, Streptomyces erythraeus Tü4015 and Streptomyces albogriseolus They can inhibit the growth of tumor cells and induce tumor cell apoptosis. They have strong inhibitory activity on MCF7, K562, P388 and other tumor cell lines, and also have certain inhibition on Gram-positive and negative bacteria. effect. (Sato T et al: J. Antibiot., 1982, 35 (3), 266-271; Morimoto M et al: J. Antibiot., 1985, 38 (4), 490-495; Dübeler A et al: Eur. J. Org. Chem., 2002, 983-987; Cui CB et al: Fitoterapia, 2007, 78(3), 238-240). [0003] T...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P17/18C12N1/20C12R1/01
CPCC12N1/20C12P17/181C12N1/205C12R2001/01
Inventor 李一青许歆栋赵立兴苗翠苹陈有为徐丽华
Owner YUNNAN UNIV
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