45 results about "Lovastatine" patented technology

The invention relates to the technical field of microorganisms, in particular to monascus for high-yield production of monascus and / or monascus flavin and application of the monascus, a new monascus strain with the preservation number of CGMCC NO.41335 and application of the new monascus strain in production of high-content monascus and / or monascus flavin and related products thereof. The strain is monascus purpureus ZYXQ-2206, can produce high-efficiency and non-toxic monascus and / or monascus flavin at high yield, and does not produce harmful and toxic components such as lovastatin and citrinin. Therefore, a monascus product produced by using the strain can effectively avoid safety risks, and has extremely high application prospects and market potential. By screening and optimizing a fermentation process, the invention provides the high-yield, non-toxic and high-functionality red yeast rice, and the red yeast rice has important application value in the field of development of lipid-lowering functional foods and nutritional health-care products.

The invention discloses a compound fish oil emulsion for preventing cardiovascular diseases and a preparation process thereof, and relates to the technical field of fish oil emulsions. Comprising the following raw materials: fish oil, rice bran fatty alkanol, a blood protein peptide concentrated solution, red yeast rice, haws, lotus leaves, flammulina velutipes extract powder, sorbitol, glycerin, citric acid fatty acid ester, monoglyceride and diglyceride, sweet orange oil, lemon oil and vitamins. According to the compound fish oil emulsion for preventing cardiovascular diseases and the preparation process thereof, the fish oil is beneficial to blood fat regulation, the red yeast rice contains lovastatin and can reduce cholesterol, and the hawthorn and the lotus leaves assist in reducing blood fat and act together with the fish oil, so that the blood fat regulation effect is enhanced, the blood viscosity is reduced, and the risk of cardiovascular diseases is reduced; the fish oil provides Omega-3 fatty acid, the blood protein peptide concentrated solution provides protein, and the rice bran fatty alkanol has nutritional and health-care effects, so that the nutritional value of the product is increased through synergism of the fish oil, the blood protein peptide concentrated solution and the rice bran fatty alkanol, the human immunity is enhanced, and the body function is improved.

The invention discloses medicinal and edible monascus purpureus with high yield of lovastatin and application of the medicinal and edible monascus purpureus, and belongs to the technical field of microbial strains and fermentation engineering. The monascus purpureus M230530Z3Db6a has the following remarkable advantages that the monascus purpureus M230530Z3Db6a screened by the monascus purpureus M230530Z3Db6a has high yield of lovastatin, and the strain is safe and non-toxic; 3) the content of lovastatin can be obviously increased in the culture medium added with exogenous additives (including but not limited to glycine, galactose, burdock fructo-oligosaccharide, ammonium sulfate, tartaric acid, citric acid, ascorbic acid and other amino acids, organic acids and polysaccharides); 4) in the fermentation process of the traditional Chinese medicinal materials including the dried orange peel, the content of functional components such as total flavonoids can be remarkably increased, and the oxidation resistance is enhanced; the monascus purpureus M230530Z3Db6a has the advantages that the nutritional value of a traditional product can be improved, the product is endowed with a unique health attribute, important strain resources and technical support are provided for innovative development of monascus purpureus related industries, and the monascus purpureus M230530Z3Db6a has a wide application prospect.

The present invention relates to the field of microbial technology, and in particular to Monascus and its application in mixed bacteria fermentation technology. The present invention separates a strain of Monascus purpurogenus with a preservation number of CGMCC No.41415 and a strain of Monascus fusca with a preservation number of CGMCC No.41287, and compound the two to form a composite bacteria. They are applied to highland barley fermentation to produce pure natural and pollution-free Monascus pigment and can also metabolize to produce lovastatin with blood lipid lowering effect. The fermented highland barley red yeast not only retains the nutritional components of highland barley, but also increases the content of functional active substances in the highland barley red yeast, increases the health care effect of the highland barley red yeast, and is more suitable for the demand of modern consumers for high-quality products.

The invention relates to a gulcomannan modified mesoporous dopamine targeted multifunctional nano diagnosis and treatment agent and preparation and application thereof.The targeted multifunctional nano diagnosis and treatment agent comprises a carrier and lovastatin, the carrier is a manganese ion coordinated mesoporous polydopamine carrier, and the surface of the carrier is modified with gulcomannan. The diagnosis and treatment agent has a good drug loading rate and a good delivery effect, overcomes the defect of low oral absorption rate of single lovastatin, can carry out MRI imaging monitoring, and provides a new thought for integration of diagnosis and treatment of liver cirrhosis.

The invention provides a monascus strain, monascus, monascus decoction pieces, a preparation method of monascus, a monascus extract and a medicine. The Latin chemical name of the monascus purpureus strain is Monascus purpureus, and the preservation number of the monascus purpureus strain is CGMCC (China General Microbiological Culture Collection Center) No.40777. In a monascus product prepared by fermentation of the monascus strain provided by the invention, the content of lovastatin compounds and the content of extracts are high, and the monascus product contains polyhydroxy sterol and flavonoid components and is good in safety, so that the pharmaceutical activity and quality of the monascus product are improved.

A method for increasing the content of lovastatin in functional red yeast rice, comprising the steps of pretreatment, inoculation and fermentation. During pretreatment, rice and an auxiliary solution accounting for 5-15% of the mass of the rice are mixed and then sterilized to serve as a rice culture medium for later use, wherein the auxiliary solution contains corn steep liquor, monopotassium phosphate, and magnesium sulfate heptahydrate, and the pH value of the auxiliary solution is 4.5-5.5; and monascus is activated and then inoculated to the rice culture medium, and fermentation is carried out, thereby obtaining a red yeast rice product. According to the method, an added auxiliary solution and rice are used to prepare a unique rice culture medium, and specific variable-temperature fermentation and turning processes are adopted in combination, so that the content of lovastatin in a functional red yeast rice product can be effectively increased, and lovastatin in the product is mainly present in an acid structure.

The invention provides monascus purpureus and application thereof. The invention provides monascus purpureus XK-G-1 which is preserved in the China Center for Type Culture Collection, and the preservation number of the monascus purpureus XK-G-1 is CCTCC (China Center for Type Culture Collection) NO: M20232397. The invention also provides a leavening agent which contains the monascus purpureus. The invention further provides red yeast rice decoction pieces and red yeast rice, the red yeast rice decoction pieces and the red yeast rice are prepared from the leavening agent, and the content of ring-opening lovastatin in the red yeast rice decoction pieces is 15-17mg / g; the total color value of the red yeast rice is 3655-3855 u / g, and the color value of the orange pigment is 1300-1426 u / g. The content of ring-opening lovastatin in the red yeast rice decoction pieces prepared by the method is stabilized at 15-17mg / g, citrinin is not detected, and the red yeast rice decoction pieces are stable in quality and safe. The monascus purpureus and the leavening agent thereof are suitable for various fermentation substrates and compositions thereof such as rice, # imgabs0 oat, highland barley, maize and sticky rice, the fermentation substrates are wide, and a new choice is provided for high added value of agricultural and sideline products.

The invention discloses a production method for improving the ring opening rate of monascus milovastatin, and belongs to the technical field of monascus rice production. According to the method, a monascus purpureus high-yield strain is selected and inoculated into a solid indica rice matrix containing a specific additive for fermentation after spore activation, and the matrix comprises 1, 5-p-coumaroylquinic acid, sodium acetate, sodium citrate, a thermally modified soybean protein isolate polysaccharide sulfate compound and other components. Wherein sodium acetate and sodium citrate can serve as short-chain fatty acid precursors, the activity of closed-loop lactonase is inhibited through pH regulation, and generation of lactone type products is reduced; 1, 5-p-coumaroylquinic acid activates the expression of a polyketide synthase gene cluster, so that the synthesis flux of the Monacolin K with an open-loop structure is improved; nicotinamide ribose enhances the electron transfer efficiency of glycolysis and TCA circulation, provides sufficient ATP for polyketone synthesis, and promotes synthesis of Monacolin K. The compound further strengthens the synthesis flux of secondary metabolites by changing the form of hyphae and continuously releasing a nitrogen / carbon source, and promotes intracellular products to be transferred to extracellular.

A method is provided for treatment of atherosclerotic cardiovascular disease in a subject in need thereof by administering a statin pharmaceutical composition to the subject when the subject is qualified for over-the-counter access to the statin pharmaceutical composition. In some embodiments, the statin pharmaceutical composition includes atorvastatin, fluvastatin, lovastatin, pitavastatin, pravastatin, rosuvastatin, or simvastatin as an active ingredient.

A method of treating a wound may include topically administering a statin composition including a statin selected from atorvastatin, fluvastatin, lovastatin, pitavastatin, pravastatin, rosuvastatin, simvastatin, or combination thereof. The statin composition may be administered in an ointment, powder, or liquid format. The method may also include topically administering one or more antimicrobial drugs.

The invention belongs to the technical field of biological medicines, and particularly relates to terpenoids as well as a preparation method and application thereof. The compound is derived from a secondary metabolite of Irpex lacteus, the chemical name of the compound is determined as (6S, 6aS, 9aR, Z)-6, 8, 8-trimetyl-6, 6a, 7, 8, 9, 9a-hexahydrozoulene [5, 4-c] furan-3, 5 (1H, 4H)-dione through a nuclear magnetic resonance hydrogen spectrum, a carbon spectrum, a two-dimensional spectrum, a high-resolution mass spectrum, an infrared spectrogram and the like, and the chemical structure of the compound is shown as follows: # imgabs 0 # database screening shows that the compound is a novel terpenoid compound, and the compound has a wide application prospect. The strain is separated from Irpex lacteus leavening for the first time. A biological activity test shows that the compound has relatively strong triglyceride reduction activity, and the effect of the compound is superior to that of a common drug lovastatin. Therefore, the novel terpenoid has a wide application prospect in the aspects of medicinal or non-medicinal lipid reduction and blood fat reduction.

The invention belongs to the technical field of biological medicine, and particularly relates to application of lovastatin combined with DHCR7 inhibitor AY9944 in preparation of medicine for treating colorectal cancer. The DHCR7 protein expression in the drug-resistant tumor tissue is obviously improved compared with that of normal tumor cells, and verification finds that the DHCR7 inhibitor AY9944 can specifically inhibit the drug resistance of colorectal cancer cells generating drug resistance, and further finds that when AY9944 and lovastatin are combined for use at a specific concentration ratio, the drug resistance of the colorectal cancer cells generating drug resistance can be inhibited, and the drug resistance of the colorectal cancer cells generating drug resistance can be inhibited. The two can play an obvious synergistic effect, so that the sensitivity of intestinal cancer cells to oxaliplatin is more effectively improved. Therefore, the combined medication mode provided by the invention can provide a new strategy reference for clinically overcoming the chemotherapy resistance of colorectal cancer.

The invention discloses a preparation method of an ultrathin corrosion-resistant composite coating modified magnesium-based material, and belongs to the technical field of biomedical materials. According to the method, a layer of heparin / lovastatin-IPP coating is prepared on the surface of the AZ31 magnesium alloy. In the growth process of a film on the surface of a magnesium matrix, negative bias voltage is applied to a substrate in a pulse mode, enhanced high-energy ion bombardment can be provided in the film polymerization process, high-concentration free radicals maintaining long-time activity are generated in a plasma polymerization structure, and the free radicals can be used for covalent binding with other functional biomolecules. The IPP coating obviously improves the corrosion resistance of the AZ31 magnesium alloy matrix, and a large number of reactive free radicals are distributed on the surface of the coating. Through a simple and convenient soaking method, the free radicals can be combined with heparin or lovastatin with good biological functionality, so that the ultrathin composite coating with corrosion resistance and anticoagulation, anti-inflammatory and endothelialization functional potentials is formed.

The invention discloses a method for efficiently detecting trace residues of'three-high 'drugs in fish plasma, the'three-high' drugs comprise triamterene, terazosin, rosiglitazone, lovastatin, benazepril, pitavastatin and repaglinide, and the method comprises the following steps: adding an anticoagulant into a collected fish plasma sample, centrifuging, taking supernate, and collecting the supernate; the method comprises the following steps: firstly, adding an internal standard solution, adding acetone containing formic acid with the volume fraction of 1% into a purification tube, loading a labeled sample, pressurizing to ensure that the sample drips in a drop shape, collecting by using a polypropylene test tube containing a purification reagent, oscillating and purifying, centrifuging, taking supernate, and concentrating to obtain a concentrated solution; the method disclosed by the invention is high in sensitivity, and the detection limit range of seven kinds of drugs for target'three highs' in the blood plasma of the aquatic environment fish is 0.02-0.25 ng / mL.

The invention discloses equipment for improving the stability of lovastatin in a red yeast rice compound and a preparation method of the equipment, relates to the technical field of lovastatin preparation equipment structures, and aims to solve the problem that the use effect of red yeast rice is affected due to poor stability of lovastatin in an existing red yeast rice compound. A wet granulation box is mounted above the supporting box, a mounting cover is mounted above the wet granulation box, a mixing device is mounted above the mounting cover, a sealing cover is mounted above the mixing device, a stirrer is mounted above the sealing cover, a supporting rod is mounted above a first mounting frame, and a second mounting frame is mounted above the supporting rod. A supporting rod is installed on the upper portion of the first mounting frame, a mounting base is installed on the upper portion of the supporting rod, an air cylinder is installed on the upper portion of the mounting base, a tabletting box is installed in the first mounting frame, a supporting plate is installed on one side of the tabletting box, a second mounting frame is installed on one side of the first mounting frame, and a spiral conveying device is installed in the second mounting frame.

Provided in the present application are a Monascus strain, a red yeast rice, a red yeast rice decoction piece, a preparation method for red yeast rice, a red yeast rice extract and a drug. The Latin name of the Monascus strain is Monascus purpureus, and the accession number thereof is CGMCC No. 40777. A red yeast rice product prepared by means of fermentation using the Monascus strain of the present application has a high content of lovastatin compounds and a high content of extractives, contains polyhydroxy sterol and flavonoid components and has good safety, thus improving the drug activity and quality of red yeast rice products.

A method of treating a wound may include topically administering a statin composition including a statin selected from atorvastatin, fluvastatin, lovastatin, pitavastatin, pravastatin, rosuvastatin, simvastatin, or combination thereof. The statin composition may be administered in an ointment, powder, or liquid format. The method may also include topically administering one or more antimicrobial drugs and / or naltrexone.

To provide a novel prophylactic or therapeutic for GM1 gangliosidosis, and a novel composition for preventing or treating GM1 gangliosidosis.SOLUTION: Provided is a prophylactic or therapeutic for GM1 gangliosidosis, comprising, as an active ingredient, at least one selected from the group consisting of amodiaquine, acacetin, sulfamerazine, ungerine, amiodarone, sertindole, delcorine, perphenazine, althiazide, diethylstilbestrol, thiethylperazine, harmol, skimmianine, succinylsulfathiazole, phillalbine, canavanine, harmaline, trihexyphenidyl, fluoxetine, lovastatin, haloperidol, prenylamine lactate, bromperidol, convolvamine, and miglustat, and pharmaceutically acceptable salts thereof, or solvates thereof.SELECTED DRAWING: Figure 5

This invention proposes an analytical method for evaluating the quality of red yeast rice based on HPLC fingerprinting and multi-component quantitative analysis, belonging to the field of quality control technology for red yeast rice and related products. The method includes: taking a red yeast rice sample, ultrasonically extracting it with 50% methanol, centrifuging, and filtering to prepare a test solution; performing detection using high-performance liquid chromatography; constructing a red yeast rice fingerprint chromatogram based on the detection results, and simultaneously determining the content of six active ingredients: ankaflavin, citrinin, lactone-type mevastatin, lactone-type lovastatin, acid mevastatin, and acid lovastatin; and combining chemical pattern recognition methods to perform discriminant analysis on red yeast rice samples from different sources to evaluate the quality of the red yeast rice. This invention achieves an organic combination of simultaneous quantitative analysis of multiple components in red yeast rice and overall quality evaluation based on fingerprint chromatograms. Methodological validation shows that this method has good precision, repeatability, and stability, and is accurate and reliable, suitable for the quality control of red yeast rice raw materials and health foods and pharmaceuticals containing red yeast rice.

A system for manufacturing a Pulsincap dosage form containing lovastatin and colesevelam for the treatment of hypercholesterolemia, comprising: a) a fungal cultivation facility configured to cultivate mangrove-associated marine fungi from the group consisting of Aspergillus sp., Penicillium sp., Rhizophus sp. and Fusarium sp. in production media to produce the metabolite lovastatin; b) a microsphere preparation plant for the production of floating lovastatin microspheres using ionotropic gelation technology with chitosan, sodium alginate and calcium carbonate; c) a granulation plant for the production of colesevelam granules using explosives from the group consisting of croscarmellose and microcrystalline cellulose; d) a capsule coating unit configured to coat the capsule body with cellulose acetate phthalate (CAP); and e) a stopper preparation unit configured to prepare hydrogel stoppers and coat them with ethylcellulose.

The invention provides monascus purpureus, a high-yield lovastatin and low-citrinin culture medium and a method, and belongs to the technical field of microbial fermentation. The method comprises the following steps: performing streak inoculation on Monascus purpureus HBSDYX-026 to a seed culture medium, and culturing to obtain a seed solution; and inoculating the seed solution to a high-yield lovastatin culture medium for fermentation. According to the invention, by optimizing the components of the culture medium, adjusting the variety and proportion of key nutrient substances, namely a carbon source and a nitrogen source, and screening and adding traditional Chinese medicines and an accelerant, a more suitable environment is provided for the growth of monascus and the synthesis of lovastatin; a monascus strain which is stable in heredity, vigorous in growth, high in lovastatin yield and low in citrinin yield is obtained, a culture medium which can efficiently synthesize lovastatin and is low in citrinin is successfully constructed, and the yield of lovastatin in red yeast rice is remarkably increased.

The invention relates to the technical field of yellow rice wine, in particular to coix seed red yeast rice yellow rice wine with high antioxidant activity as well as a preparation method and application thereof. The monascus ruber (3.15746) is used for carrying out microbial fermentation conversion on the coix seeds, and the coix seed and monascus ruber yellow rice wine is short in production period, excellent in sensory quality, rich in lovastatin and high in antioxidant activity. The monascus yellow wine strain is enriched, the content of active ingredients and the health-care effect of the monascus yellow wine are improved, and a feasible scheme and technical parameters can be provided for industrial development.

This invention discloses a medicament for inhibiting the formation of thorns in Sichuan pepper, relating to the field of Sichuan pepper cultivation technology. The medicament comprises the following components at final concentrations: lovastatin 5-20 mg / L, 6-mercaptopurine 5-10 mg / L, pyrimidinol 10-30 mg / L, proline 20-100 mg / L, glycine 20-100 mg / L, glutamic acid 20-100 mg / L, potassium dihydrogen phosphate 5-7 mg / L, sucrose 5-7 mg / L, and Tween-80 1-2 mg / L. This invention also provides a method for preparing the medicament for inhibiting the formation of thorns in Sichuan pepper. This invention reduces the incidence of thorns in Sichuan pepper, with a significant thorn-inhibiting effect. While inhibiting thorn formation, it also increases the growth of new shoots in Sichuan pepper, effectively solving the problem that single-hormone inhibitors easily lead to excessive shoot growth, decreased flower bud differentiation rate, or reduced fruit weight.

The application discloses a method for establishing a Hongqu fingerprint spectrum by using a high performance liquid chromatography technology and application of the method in quality detection of Shiguogong medicinal liquor, the Hongqu fingerprint spectrum obtained by using the method has good separation of each chromatographic peak, a smooth baseline, a good peak type, and good precision, repeatability, specificity and stability, seven fingerprint peaks of the Hongqu fingerprint spectrum established by the application are confirmed, and by comparison with reference substances and in combination with ultraviolet absorption spectrum characteristics, characteristic peaks of main chemical components, namely, open-loop lovastatin and lovastatin in the Hongqu are identified, the method can be used for quality detection of the Hongqu, and provides a scientific basis for improving a production process and quality control of the Hongqu, and can also be applied to quality detection of Hongqu components in the Shiguogong medicinal liquor, and has important significance for guaranteeing the quality of the Hongqu in the Shiguogong medicinal liquor.

The invention belongs to the technical field of medicines, and relates to a lovastatin liposome as well as a preparation method and application thereof, in particular to a lovastatin liposome as well as a preparation method and application thereof in preparation of antitumor drugs. The liposome comprises lovastatin, phospholipid, cholesterol and lauryl sodium sulfate, the mass ratio of lovastatin to phospholipid is (1: 15)-(1: 30), the mass ratio of cholesterol to phospholipid is (1: 5)-(1: 20), and the mass ratio of lovastatin to lauryl sodium sulfate is 1: (0.5-1). The lovastatin liposome is small in particle size, high in encapsulation efficiency and good in stability, has a certain slow release effect, not only can improve the bioavailability of drugs, but also has an obvious inhibition effect on tumor cells. Particularly, the inhibition effect on colorectum is optimal.

The embodiment of the invention discloses a method for determining trace acid and lactone lovastatin in red yeast rice food through solid phase extraction-liquid chromatography-tandem mass spectrometry, and belongs to the technical field of analysis and detection. According to the method, a formic acid acetonitrile solution with the volume fraction of 1% is used as an extraction solvent to perform pretreatment on a sample to be detected, solid-phase extraction and purification are performed, and qualitative and quantitative analysis is performed by adopting liquid chromatography-tandem mass spectrometry. The acid lovastatin and lactone lovastatin separation degree is good, high sensitivity and accuracy are achieved, the detection limit is low (the detection limit of acid lovastatin is 0.099 micrograms per kilogram, and the detection limit of lactone lovastatin is 0.084 micrograms per kilogram), a trace amount of target objects can be accurately quantified, and the method is suitable for large-scale industrial production. And reliable technical support is provided for quality control and standardized detection of trace lovastatin in the red yeast rice product.

The invention discloses a construction method of an aspergillus niger strain for efficient heterologous synthesis of Monacolin J. According to the invention, in saccharomyces cerevisiae BY4741, a transformation coupled recombination (TAR) technology is utilized to construct an expression vector of a lovastatin precursor Monacolin J, wherein the expression vector is formed by serially connecting lovB, lovC, lovG and lovA genes regulated and controlled by a strong promoter and a strong terminator of filamentous fungi. According to the invention, the steps of expression vector construction and aspergillus niger homologous recombination transformation are simplified, the lovastatin is efficiently and heterologously synthesized in the aspergillus niger by optimizing fermentation components, and a research idea is provided for the construction of a macromolecular vector and the research of metabolic engineering and synthetic biology of filamentous fungi.

The invention discloses a method for determining the content of statin components in a red yeast rice product based on quantitative analysis of multi-components by single marker and application of the method. The method comprises the following steps: (1) preparing a lovastatin reference substance into a reference substance solution; (2) obtaining an alcohol extract of a monascus product to be detected as a test solution; (3) respectively carrying out high performance liquid chromatography detection on the test solution and the reference solution, and calculating the content of lovastatin in the test solution through an external standard method; (4) respectively calculating the content of lovastatin acid and the content of dehydroxylated lovastatin in the test sample according to a formula: Ci = Ai * CS / (fi / s * AS) and the determined correction factor fi / s by taking the content of lovastatin in the test sample as a reference; the fi / s value of the lovastatin acid is 0.6229, and the fi / s value of the hydroxyl-removed lovastatin is 1.1479. The method is rapid, simple and convenient, low in cost and very accurate in result.